The Aberrant DNA Methylation Profile of Human Induced
Pluripotent Stem Cells Is Connected to the Reprogramming
Process and Is Normalized During In Vitro Culture

J 2016

The Aberrant DNA Methylation Profile of Human Induced Pluripotent Stem Cells Is Connected to the Reprogramming Process and Is Normalized During In Vitro Culture

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ

Základní údaje

Originální název

The Aberrant DNA Methylation Profile of Human Induced Pluripotent Stem Cells Is Connected to the Reprogramming Process and Is Normalized During In Vitro Culture

Autoři

TESAŘOVÁ, Lenka (203 Česká republika, domácí), Pavel ŠIMARA (203 Česká republika, domácí), Stanislav STEJSKAL (203 Česká republika, domácí) a Irena KRONTORÁD KOUTNÁ (203 Česká republika, garant, domácí)

Vydání

PLOS ONE, Public Library of Science, 2016, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.806

Kód RIV

RIV/00216224:14330/16:00088006

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1371/journal.pone.0157974

UT WoS

000378389200077

Klíčová slova anglicky

pluripotent stem cells; DNA methylation; MethylScreen technology; CpG islands; pluripotency and differentiation markers

Štítky

cbia-web

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 2. 2021 15:23, Mgr. Tereza Miškechová

Anotace

V originále

The potential clinical applications of human induced pluripotent stem cells (hiPSCs) are limited by genetic and epigenetic variations among hiPSC lines and the question of their equivalency with human embryonic stem cells (hESCs). We used MethylScreen technology to determine the DNA methylation profile of pluripotency and differentiation markers in hiPSC lines from different source cell types compared to hESCs and hiPSC source cells. After derivation, hiPSC lines compromised a heterogeneous population characterized by variable levels of aberrant DNA methylation. These aberrations were induced during somatic cell reprogramming and their levels were associated with the type of hiPSC source cells. hiPSC population heterogeneity was reduced during prolonged culture and hiPSCs acquired an hESC-like methylation profile. In contrast, the expression of differentiation marker genes in hiPSC lines remained distinguishable from that in hESCs. Taken together, in vitro culture facilitates hiPSC acquisition of hESC epigenetic characteristics. However, differences remain between both pluripotent stem cell types, which must be considered before their use in downstream applications.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

LM2015090, projekt VaV

Název: Český národní uzel Evropské sítě infrastruktur klinického výzkumu (Akronym: CZECRIN)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CZECRIN - Český národní uzel Evropské sítě infrastruktur klinického výzkumu

Citovat

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. The Aberrant DNA Methylation Profile of Human Induced Pluripotent Stem Cells Is Connected to the Reprogramming Process and Is Normalized During In Vitro Culture. PLOS ONE. Public Library of Science, 2016, roč. 11, č. 6, s. nestránkováno, 16 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0157974.

@article{1348695,
   author = {Tesařová, Lenka and Šimara, Pavel and Stejskal, Stanislav and Krontorád Koutná, Irena},
   article_number = {6},
   doi = {http://dx.doi.org/10.1371/journal.pone.0157974},
   keywords = {pluripotent stem cells; DNA methylation; MethylScreen technology; CpG islands; pluripotency and differentiation markers},
   language = {eng},
   issn = {1932-6203},
   journal = {PLOS ONE},
   title = {The Aberrant DNA Methylation Profile of Human Induced Pluripotent Stem Cells Is Connected to the Reprogramming Process and Is Normalized During In Vitro Culture},
   url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157974},
   volume = {11},
   year = {2016}
}

TY  - JOUR
ID  - 1348695
AU  - Tesařová, Lenka - Šimara, Pavel - Stejskal, Stanislav - Krontorád Koutná, Irena
PY  - 2016
TI  - The Aberrant DNA Methylation Profile of Human Induced Pluripotent Stem Cells Is Connected to the Reprogramming Process and Is Normalized During In Vitro Culture
JF  - PLOS ONE
VL  - 11
IS  - 6
SP  - nestránkováno
EP  - nestránkováno
PB  - Public Library of Science
SN  - 19326203
KW  - pluripotent stem cells
KW  - DNA methylation
KW  - MethylScreen technology
KW  - CpG islands
KW  - pluripotency and differentiation markers
UR  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157974
L2  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157974
N2  - The potential clinical applications of human induced pluripotent stem cells (hiPSCs) are limited by genetic and epigenetic variations among hiPSC lines and the question of their equivalency with human embryonic stem cells (hESCs). We used MethylScreen technology to determine the DNA methylation profile of pluripotency and differentiation markers in hiPSC lines from different source cell types compared to hESCs and hiPSC source cells. After derivation, hiPSC lines compromised a heterogeneous population characterized by variable levels of aberrant DNA methylation. These aberrations were induced during somatic cell reprogramming and their levels were associated with the type of hiPSC source cells. hiPSC population heterogeneity was reduced during prolonged culture and hiPSCs acquired an hESC-like methylation profile. In contrast, the expression of differentiation marker genes in hiPSC lines remained distinguishable from that in hESCs. Taken together, in vitro culture facilitates hiPSC acquisition of hESC epigenetic characteristics. However, differences remain between both pluripotent stem cell types, which must be considered before their use in downstream applications.
ER  -

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL a Irena KRONTORÁD KOUTNÁ. The Aberrant DNA Methylation Profile of Human Induced Pluripotent Stem Cells Is Connected to the Reprogramming Process and Is Normalized During In Vitro Culture. \textit{PLOS ONE}. Public Library of Science, 2016, roč.~11, č.~6, s.~nestránkováno, 16 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0157974.

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