Serum-Based MicroRNA Signatures in Early Diagnosis and
Prognosis Prediction of Colon Cancer

J 2016

Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer

VYCHYTILOVÁ, Petra, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka KOSAŘOVÁ, Kateřina SLABÁ et. al.

Základní údaje

Originální název

Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer

Autoři

VYCHYTILOVÁ, Petra (203 Česká republika, domácí), Lenka RADOVÁ (203 Česká republika, domácí), Milana ŠACHLOVÁ (203 Česká republika, domácí), Zdeňka KOSAŘOVÁ (203 Česká republika, domácí), Kateřina SLABÁ (203 Česká republika, domácí), Pavel FABIAN (203 Česká republika, domácí), Tomáš GROLICH (203 Česká republika, domácí), Vladimír PROCHÁZKA (203 Česká republika, domácí), Zdeněk KALA (203 Česká republika, domácí), Marek SVOBODA (203 Česká republika, domácí), Igor KISS (203 Česká republika, domácí), Rostislav VYZULA (203 Česká republika, domácí) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

Carcinogenesis, Oxford, Oxford University Press, 2016, 0143-3334

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.105

Kód RIV

RIV/00216224:14740/16:00088870

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1093/carcin/bgw078

UT WoS

000386037100003

Klíčová slova česky

mikroRNA; kolorektální karcinom; krevní sérum; diagnostický biomarker; prognostický biomarker

Klíčová slova anglicky

microRNA; colorectal cancer; blood serum; diagnostic biomarker; prognostic biomarker

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 4. 2021 13:39, Mgr. Tereza Miškechová

Anotace

V originále

Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of the present study was to identify serum microRNAs enabling early diagnosis and prognosis prediction of colon cancer. In total, serum samples from 427 colon cancer patients and 276 healthy donors were included in 3-phase biomarker study. Large-scale microRNA expression profiling was performed using Illumina small RNA sequencing. Diagnostic and prognostic potential of identified microRNAs was validated on independent training and validation sets of samples using RT-qPCR. Fifty-four microRNAs were found to be significantly deregulated in serum of colon cancer patients compared to healthy donors (P < 0.01). A diagnostic 4-microRNA signature consisting of miR-23a-3p, miR-27a-3p, miR-142-5p and miR-376c-3p was established (AUC = 0.917), distinguishing colon cancer patients from healthy donors with sensitivity of 89% and specificity of 81% (AUC = 0.922). This panel of microRNAs exhibited high diagnostic performance also when analyzed separately in colon cancer patients in early stages of the disease (T1-4N0M0; AUC = 0.877). Further, a prognostic panel based on the expression of miR-23a-3p and miR-376c-3p independent of TNM stage was established (HR 2.30; 95% CI 1.44-3.66; P < 0.0004). In summary, highly sensitive signatures of circulating microRNAs enabling non-invasive early detection and prognosis prediction of colon cancer were identified.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NT13549, projekt VaV

Název: Vytvoření diagnostické sady cirkulujících mikroRNA pro neinvazivní časnou diagnostiku a sledování pacientů s kolorektálním karcinomem

NV16-31765A, projekt VaV

Název: Využití tkáňových/cirkulujících mikroRNA pro predikci léčebné odpovědi a zpřesnění restagingu karcinomu rekta po neoadjuvantní léčbě

90004, velká výzkumná infrastruktura

Název: BBMRI-CZ

Přiložené soubory

1351259.pdf

Požádat o autorskou verzi souboru

Citovat

VYCHYTILOVÁ, Petra, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka KOSAŘOVÁ, Kateřina SLABÁ, Pavel FABIAN, Tomáš GROLICH, Vladimír PROCHÁZKA, Zdeněk KALA, Marek SVOBODA, Igor KISS, Rostislav VYZULA a Ondřej SLABÝ. Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer. Carcinogenesis. Oxford: Oxford University Press, 2016, roč. 37, č. 10, s. 941-950. ISSN 0143-3334. Dostupné z: https://dx.doi.org/10.1093/carcin/bgw078.

@article{1351259,
   author = {Vychytilová, Petra and Radová, Lenka and Šachlová, Milana and Kosařová, Zdeňka and Slabá, Kateřina and Fabian, Pavel and Grolich, Tomáš and Procházka, Vladimír and Kala, Zdeněk and Svoboda, Marek and Kiss, Igor and Vyzula, Rostislav and Slabý, Ondřej},
   article_location = {Oxford},
   article_number = {10},
   doi = {http://dx.doi.org/10.1093/carcin/bgw078},
   keywords = {microRNA; colorectal cancer; blood serum; diagnostic biomarker; prognostic biomarker},
   language = {eng},
   issn = {0143-3334},
   journal = {Carcinogenesis},
   title = {Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer},
   url = {https://academic.oup.com/carcin/article-abstract/37/10/941/2196570/Serum-based-microRNA-signatures-in-early-diagnosis?redirectedFrom=fulltext},
   volume = {37},
   year = {2016}
}

TY  - JOUR
ID  - 1351259
AU  - Vychytilová, Petra - Radová, Lenka - Šachlová, Milana - Kosařová, Zdeňka - Slabá, Kateřina - Fabian, Pavel - Grolich, Tomáš - Procházka, Vladimír - Kala, Zdeněk - Svoboda, Marek - Kiss, Igor - Vyzula, Rostislav - Slabý, Ondřej
PY  - 2016
TI  - Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer
JF  - Carcinogenesis
VL  - 37
IS  - 10
SP  - 941-950
EP  - 941-950
PB  - Oxford University Press
SN  - 01433334
KW  - microRNA
KW  - colorectal cancer
KW  - blood serum
KW  - diagnostic biomarker
KW  - prognostic biomarker
UR  - https://academic.oup.com/carcin/article-abstract/37/10/941/2196570/Serum-based-microRNA-signatures-in-early-diagnosis?redirectedFrom=fulltext
L2  - https://academic.oup.com/carcin/article-abstract/37/10/941/2196570/Serum-based-microRNA-signatures-in-early-diagnosis?redirectedFrom=fulltext
N2  - Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of the present study was to identify serum microRNAs enabling early diagnosis and prognosis prediction of colon cancer. In total, serum samples from 427 colon cancer patients and 276 healthy donors were included in 3-phase biomarker study. Large-scale microRNA expression profiling was performed using Illumina small RNA sequencing. Diagnostic and prognostic potential of identified microRNAs was validated on independent training and validation sets of samples using RT-qPCR. Fifty-four microRNAs were found to be significantly deregulated in serum of colon cancer patients compared to healthy donors (P < 0.01). A diagnostic 4-microRNA signature consisting of miR-23a-3p, miR-27a-3p, miR-142-5p and miR-376c-3p was established (AUC = 0.917), distinguishing colon cancer patients from healthy donors with sensitivity of 89% and specificity of 81% (AUC = 0.922). This panel of microRNAs exhibited high diagnostic performance also when analyzed separately in colon cancer patients in early stages of the disease (T1-4N0M0; AUC = 0.877). Further, a prognostic panel based on the expression of miR-23a-3p and miR-376c-3p independent of TNM stage was established (HR 2.30; 95% CI 1.44-3.66; P < 0.0004). In summary, highly sensitive signatures of circulating microRNAs enabling non-invasive early detection and prognosis prediction of colon cancer were identified.
ER  -

VYCHYTILOVÁ, Petra, Lenka RADOVÁ, Milana ŠACHLOVÁ, Zdeňka KOSAŘOVÁ, Kateřina SLABÁ, Pavel FABIAN, Tomáš GROLICH, Vladimír PROCHÁZKA, Zdeněk KALA, Marek SVOBODA, Igor KISS, Rostislav VYZULA a Ondřej SLABÝ. Serum-Based MicroRNA Signatures in Early Diagnosis and Prognosis Prediction of Colon Cancer. \textit{Carcinogenesis}. Oxford: Oxford University Press, 2016, roč.~37, č.~10, s.~941-950. ISSN~0143-3334. Dostupné z: https://dx.doi.org/10.1093/carcin/bgw078.

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