FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA, David KOVÁŘ, Karel LACINA a Petr SKLÁDAL. Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment. Electroanalysis. Weinheim: Wiley, 2016, roč. 28, č. 8, s. 1803-1809. ISSN 1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201600093. |
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@article{1351272, author = {Farka, Zdeněk and Juřík, Tomáš and Pastucha, Matěj and Kovář, David and Lacina, Karel and Skládal, Petr}, article_location = {Weinheim}, article_number = {8}, doi = {http://dx.doi.org/10.1002/elan.201600093}, keywords = {Atomic force microscopy; Biosensor; Immunosensor; Label-free detection; Salmonella Typhimurium}, language = {eng}, issn = {1040-0397}, journal = {Electroanalysis}, title = {Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment}, url = {http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full}, volume = {28}, year = {2016} }
TY - JOUR ID - 1351272 AU - Farka, Zdeněk - Juřík, Tomáš - Pastucha, Matěj - Kovář, David - Lacina, Karel - Skládal, Petr PY - 2016 TI - Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment JF - Electroanalysis VL - 28 IS - 8 SP - 1803-1809 EP - 1803-1809 PB - Wiley SN - 10400397 KW - Atomic force microscopy KW - Biosensor KW - Immunosensor KW - Label-free detection KW - Salmonella Typhimurium UR - http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full L2 - http://onlinelibrary.wiley.com/doi/10.1002/elan.201600093/full N2 - A label-free immunosensor for rapid detection of Salmonella Typhimurium based on electrochemical impedance spectroscopy was developed. Specific antibody was immobilized to a screen-printed electrode via cysteamine monolayer activated with glutaraldehyde and the impedance was measured between two gold electrodes. Different procedures for sample treatment (combinations of heat and sonication) were tested and their impact on the assay performance was compared. Atomic force microscopy was used to study the effect of the treatment on the cell shape and to confirm the specific binding of Salmonella to the sensing surface. The immunosensor allowed detection of 1×10^3 CFU·mL-1 in 20 min with negligible interference from other bacteria. Wide linear response was obtained in the range between 10^3 CFU·mL-1 and 10^8 CFU·mL-1. The successful detection of Salmonella in spiked milk demonstrates the suitability of sensor for the analysis of real samples. ER -
FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA, David KOVÁŘ, Karel LACINA a Petr SKLÁDAL. Rapid Immunosensing of Salmonella Typhimurium Using Electrochemical Impedance Spectroscopy: the Effect of Sample Treatment. \textit{Electroanalysis}. Weinheim: Wiley, 2016, roč.~28, č.~8, s.~1803-1809. ISSN~1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201600093.
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