Analysis of gamma-delta T cells and plasmacytoid dendritic
cells in MGUS and Multiple Myeloma patients

k 2016

Analysis of gamma-delta T cells and plasmacytoid dendritic cells in MGUS and Multiple Myeloma patients

KRÁLOVÁ, Romana, Lucie RIHOVA, Pavla VSIANSKA, Mária KRCHNIAKOVÁ, Martin PISKÁČEK et. al.

Základní údaje

Originální název

Analysis of gamma-delta T cells and plasmacytoid dendritic cells in MGUS and Multiple Myeloma patients

Autoři

KRÁLOVÁ, Romana (203 Česká republika, domácí), Lucie RIHOVA (203 Česká republika), Pavla VSIANSKA (203 Česká republika), Mária KRCHNIAKOVÁ (703 Slovensko, domácí), Martin PISKÁČEK (40 Rakousko, domácí), Rita PACASOVA (203 Česká republika), Miroslav PENKA (203 Česká republika), Zdenek ADAM (203 Česká republika), Ludek POUR (203 Česká republika), Roman HAJEK (203 Česká republika) a Andrea KNIGHT (203 Česká republika, garant, domácí)

Vydání

Gamma Delta Conference 2016, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/16:00088873

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

gamma-delta T cells; MGUS; Multiple Myeloma

Štítky

EL OK

Změněno: 16. 8. 2016 15:02, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Multiple Myeloma (MM) remains incurable B-cell malignancy despite novel therapies. Plasmacytoid dendritic cells (pDC) have been shown to mediate tumour plasma cell growth, survival and drug resistance in MM patients. The role of effector gammadelta T cell interactions with pDC in patients progressing from monoclonal gammopathy of undetermined significance (MGUS) to MM is unknown. Our aim was to determine frequencies and phenotypes of pDC together with Vdelta1 and Vdelta2 gammadelta T cells. We used bone marrow (BM) and paired peripheral blood (PB) samples from MGUS (n=8) and newly diagnosed myeloma (n=11) patients. Total of n=24 age-matched healthy donors samples have been analysed. PDCs were analysed by multicolour flow cytometry as CD123+, CD303+, and CD304+. Phenotype of active pDC was identified as HLA-DR+ CD45RA+. In MGUS and MM patients, the medians of Vdelta1 T cells were comparable to normal levels in contrast to Vdelta2 T cells being significantly decreased in both patient cohorts. In newly diagnosed MM patients, frequencies of pDCs were shown significantly reduced compared to MGUS patients (p=0.012) or normal BM (p<0.0001). PB-derived pDC from patient cohorts were numerically within the normal range. All normal pDC samples presented an active phenotype in contrast to only 50% of MGUS or MM patients. In summary, we found dramatic differences in gammadelta T cells and pDC numbers and phenotype in BM of myeloma patients. Further analyses of the key interactions between the pDC, myeloma and gammadelta T cells through functional studies, gene expression profiles in patient cohorts will be discussed.

Návaznosti

NV15-32935A, projekt VaV

Název: Analýza gamma-delta T lymfocytů reaktivních na nádorové buňky u pacientů s B-buněčnou chronickou lymfocytární leukémií: nový přístup k buněčné terapii

3SGA5924, interní kód MU

Název: Investigations of human gamma-delta T cells: a new candidate for novel antiviral and antitumour immunotherapy (Akronym: Gammadelta T cells)

Investor: Jihomoravský kraj, Investigations of human gamma-delta T cells: a new candidate for novel antiviral and antitumour immunotherapy, Reintegrační granty

Citovat

KRÁLOVÁ, Romana, Lucie RIHOVA, Pavla VSIANSKA, Mária KRCHNIAKOVÁ, Martin PISKÁČEK, Rita PACASOVA, Miroslav PENKA, Zdenek ADAM, Ludek POUR, Roman HAJEK a Andrea KNIGHT. Analysis of gamma-delta T cells and plasmacytoid dendritic cells in MGUS and Multiple Myeloma patients. In Gamma Delta Conference 2016. 2016.

@proceedings{1351326,
   author = {Králová, Romana and Rihova, Lucie and Vsianska, Pavla and Krchniaková, Mária and Piskáček, Martin and Pacasova, Rita and Penka, Miroslav and Adam, Zdenek and Pour, Ludek and Hajek, Roman and Knight, Andrea},
   booktitle = {Gamma Delta Conference 2016},
   keywords = {gamma-delta T cells; MGUS; Multiple Myeloma},
   language = {eng},
   title = {Analysis of gamma-delta T cells and plasmacytoid dendritic cells in MGUS and Multiple Myeloma patients},
   year = {2016}
}

TY  - CONF
ID  - 1351326
AU  - Králová, Romana - Rihova, Lucie - Vsianska, Pavla - Krchniaková, Mária - Piskáček, Martin - Pacasova, Rita - Penka, Miroslav - Adam, Zdenek - Pour, Ludek - Hajek, Roman - Knight, Andrea
PY  - 2016
TI  - Analysis of gamma-delta T cells and plasmacytoid dendritic cells in MGUS and Multiple Myeloma patients
KW  - gamma-delta T cells
KW  - MGUS
KW  - Multiple Myeloma
N2  - Multiple Myeloma (MM) remains incurable B-cell malignancy despite novel therapies. Plasmacytoid dendritic cells (pDC) have been shown to mediate tumour plasma cell growth, survival and drug resistance in MM patients. The role of effector gammadelta T cell interactions with pDC in patients progressing from monoclonal gammopathy of undetermined significance (MGUS) to MM is unknown. Our aim was to determine frequencies and phenotypes of pDC together with Vdelta1 and Vdelta2 gammadelta T cells. We used bone marrow (BM) and paired peripheral blood (PB) samples from MGUS (n=8) and newly diagnosed myeloma (n=11) patients. Total of n=24 age-matched healthy donors samples have been analysed. PDCs were analysed by multicolour flow cytometry as CD123+, CD303+, and CD304+. Phenotype of active pDC was identified as HLA-DR+ CD45RA+. In MGUS and MM patients, the medians of Vdelta1 T cells were comparable to normal levels in contrast to Vdelta2 T cells being significantly decreased in both patient cohorts. In newly diagnosed MM patients, frequencies of pDCs were shown significantly reduced compared to MGUS patients (p=0.012) or normal BM (p<0.0001). PB-derived pDC from patient cohorts were numerically within the normal range. All normal pDC samples presented an active phenotype in contrast to only 50% of MGUS or MM patients. In summary, we found dramatic differences in gammadelta T cells and pDC numbers and phenotype in BM of myeloma patients. Further analyses of the key interactions between the pDC, myeloma and gammadelta T cells through functional studies, gene expression profiles in patient cohorts will be discussed.
ER  -

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