Analysis of anti-tumour and anti-viral reactivities of human gamma-delta T cells

KNIGHT, Andrea, Martin PISKÁČEK, Romana KRÁLOVÁ, Mária KRCHNIAKOVÁ, Petra GALLOVÁ, Martin KUBEŠ, Lucie RIHOVA, Pavla VSIANSKA, Rita PACASOVA, Miroslav PENKA, Zdenek ADAM, Ludek POUR, Roman HAJEK and Anna VAŠKŮ. Analysis of anti-tumour and anti-viral reactivities of human gamma-delta T cells. In Gamma Delta Conference 2016. 2016.

Basic information

• Original name: Analysis of anti-tumour and anti-viral reactivities of human gamma-delta T cells
• Authors: KNIGHT, Andrea (203 Czech Republic, guarantor, belonging to the institution), Martin PISKÁČEK (40 Austria, belonging to the institution), Romana KRÁLOVÁ (203 Czech Republic, belonging to the institution), Mária KRCHNIAKOVÁ (703 Slovakia, belonging to the institution), Petra GALLOVÁ (203 Czech Republic, belonging to the institution), Martin KUBEŠ (203 Czech Republic, belonging to the institution), Lucie RIHOVA (203 Czech Republic), Pavla VSIANSKA (203 Czech Republic), Rita PACASOVA (203 Czech Republic), Miroslav PENKA (203 Czech Republic), Zdenek ADAM (203 Czech Republic), Ludek POUR (203 Czech Republic), Roman HAJEK (203 Czech Republic) and Anna VAŠKŮ (203 Czech Republic, belonging to the institution).
• Edition: Gamma Delta Conference 2016, 2016.

Other information

• Original language: English
• Type of outcome: Presentations at conferences
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/16:00090548
• Organization unit: Faculty of Medicine
• Keywords in English: gamma-delta T cells
• Tags: EL OK

Abstract

Human gd T cells are currently the subject of intensive research ana large expansions of tumour-reactive gd T cells have been observed inpatients with haematological malignancies including Multiple Myeloma (MM) and chronic leukaemias (CML, CLL) in our laboratory. However, the role of innate effector gd T cells subsets in patients progressing from monoclonal gammopathy of undetermined significance (MGUS) to MM is currently unknown. Previously, we have also shown expansion of gd T lymphocytes in cytomegalovirus (CMV) seropositive healthy donors compared to CMV seronegative individuals (p=0.0002) suggesting their direct involvement in anti-CMV immune response. Here we performed detailed analyses of expansion, phenotype, clonality and function of Vdl and Vd2 gd T cells isolated from patients and age-matched healthy donors (HD, n=53). We have analysed bone marrow (BM) and paired peripheral blood (PB) samples from MGUS (n=30) and newly diagnosed myeloma (n=52) patients. Second, we determined the whole genome profiles of tumour-reactive gd T cells and compared these to healthy donors. Third, we analysed healthy donors with five most commonly presented aileles for anti-CMV responses of gd T cells in parallel to CMV-specific ab T cells in the same HLA-A*02, HLA-A*01, HLA-A*24, HLA-A*07, and HLA-A*35 donors. The microRNA (miRNA) expression profiles have been generated from HLA-A*02, HLA-A801 CMV seropositive HD. Fourth, detailed analyses of the TCR repertoire of the gamma (g1-8, g9, g10, g11) and delta (d1-d8) chains have been determined in HD and patient cohorts. The summary of results will be presented and discussed.

Links

• MUNI/11/InGA07/2012, interní kód MU: Name: Analýza cytomegalovirové reaktivity efektorových gamma-delta buněk u zdravých jedinců

Investor: Masaryk University

• MUNI/11/InGA09/2013, interní kód MU: Name: Molekulární charakterizace gamma a delta řetězců T-buněčných receptorů a identifikace ligandů na expandovaných efektorových gamma-delta T-lymfocytech

Investor: Masaryk University

• 3SGA5924, interní kód MU: Name: Investigations of human gamma-delta T cells: a new candidate for novel antiviral and antitumour immunotherapy (Acronym: Gammadelta T cells)

Investor: South-Moravian Region, Incoming grants