Automatic Detection of Laser-Induced Structures in Live Cell
Fluorescent Microscopy Images Using Snakes with Geometric
Constraints

D 2016

Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints

KONDRAT'EV, Alexandr a Dmitry SOROKIN

Základní údaje

Originální název

Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints

Autoři

KONDRAT'EV, Alexandr (643 Rusko) a Dmitry SOROKIN (643 Rusko, garant, domácí)

Vydání

Not specified, IEEE 23rd International Conference on Pattern Recognition (ICPR), od s. 326-331, 6 s. 2016

Nakladatel

IEEE

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Kód RIV

RIV/00216224:14330/16:00088097

Organizační jednotka

Fakulta informatiky

ISBN

978-1-5090-4846-5

DOI

http://dx.doi.org/10.1109/ICPR.2016.7899655

Klíčová slova anglicky

Biological image and signal analysis; Biologically motivated vision; Segmentation features and descriptors

Štítky

CBIA, cbia-web, core_B, firank_A

Změněno: 28. 8. 2024 17:23, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

The existence of reliable evaluation datasets for cell image registration algorithms is crucial for quantitative comparison of registration approaches. A new technique for creating real live cell image sequences for this purpose was introduced recently. These datasets contain stable structures bleached by argon laser in the cell nucleus. In this work, we propose an approach for automatic detection of laser-induced linear structures in live cell fluorescent microscopy images. Compared to a previous linear laser-induced structure detection approach, our method employs an active contours model with a Hessian-based image energy term for linear structures enhancement and geometric energy term controlling the geometric relations between the structures. It uses position adaptive tension parameter values to adjust the snakes behavior in problematic regions (end points and intersection points) and a temporal consistent scheme where the results from the previous frame are used as an initial approximation for the current frame. Our approach was successfully applied to real live cell microscopy image sequences and an experimental comparison with an existing laser-induced structures detection method based on minimal paths has been performed.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

KONDRAT'EV, Alexandr a Dmitry SOROKIN. Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints. Online. In IEEE 23rd International Conference on Pattern Recognition (ICPR). Not specified: IEEE, 2016, s. 326-331. ISBN 978-1-5090-4846-5. Dostupné z: https://dx.doi.org/10.1109/ICPR.2016.7899655.

@inproceedings{1352254,
   author = {Kondrat'ev, Alexandr and Sorokin, Dmitry},
   address = {Not specified},
   booktitle = {IEEE 23rd International Conference on Pattern Recognition (ICPR)},
   doi = {http://dx.doi.org/10.1109/ICPR.2016.7899655},
   keywords = {Biological image and signal analysis; Biologically motivated vision; Segmentation features and descriptors},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Not specified},
   isbn = {978-1-5090-4846-5},
   pages = {326-331},
   publisher = {IEEE},
   title = {Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints},
   year = {2016}
}

TY  - JOUR
ID  - 1352254
AU  - Kondrat'ev, Alexandr - Sorokin, Dmitry
PY  - 2016
TI  - Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints
PB  - IEEE
CY  - Not specified
SN  - 9781509048465
KW  - Biological image and signal analysis
KW  - Biologically motivated vision
KW  - Segmentation features and descriptors
N2  - The existence of reliable evaluation datasets for cell image registration algorithms is crucial for quantitative comparison of registration approaches. A new technique for creating real live cell image sequences for this purpose was introduced recently. These datasets contain stable structures bleached by argon laser in the cell nucleus. In this work, we propose an approach for automatic detection of laser-induced linear structures in live cell fluorescent microscopy images. Compared to a previous linear laser-induced structure detection approach, our method employs an active contours model with a Hessian-based image energy term for linear structures enhancement and geometric energy term controlling the geometric relations between the structures. It uses position adaptive tension parameter values to adjust the snakes behavior in problematic regions (end points and intersection points) and a temporal consistent scheme where the results from the previous frame are used as an initial approximation for the current frame. Our approach was successfully applied to real live cell microscopy image sequences and an experimental comparison with an existing laser-induced structures detection method based on minimal paths has been performed.
ER  -

KONDRAT'EV, Alexandr a Dmitry SOROKIN. Automatic Detection of Laser-Induced Structures in Live Cell Fluorescent Microscopy Images Using Snakes with Geometric Constraints. Online. In \textit{IEEE 23rd International Conference on Pattern Recognition (ICPR)}. Not specified: IEEE, 2016, s.~326-331. ISBN~978-1-5090-4846-5. Dostupné z: https://dx.doi.org/10.1109/ICPR.2016.7899655.

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