2016
Time evolution of trapped single cell microorganism
BERNATOVÁ, Silvie, O SAMEK, S OBRUCA, M SERY, P ZEMANEK et. al.Základní údaje
Originální název
Time evolution of trapped single cell microorganism
Autoři
BERNATOVÁ, Silvie, O SAMEK, S OBRUCA, M SERY, P ZEMANEK a I MAROVA
Vydání
BELLINGHAM, IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX, od s. "SPIE"-"3", 4 s. 2016
Nakladatel
SPIE-INT SOC OPTICAL ENGINEERING
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Utajení
není předmětem státního či obchodního tajemství
ISSN
UT WoS
000378222000001
Klíčová slova anglicky
Optical tweezers; Raman spectroscopy; 2D correlation analysis; external stimuli
Změněno: 29. 8. 2016 10:09, Mgr. Silvie Bernatová, Ph.D.
Anotace
V originále
The combination of optical tweezers and Raman micro-spectroscopy is frequently referred as Raman tweezers. A single focused laser beam is utilized here both as a source of Raman scattering and a source forming an optical trap. Raman tweezers have been recently used in variety of applications in cell biology as a useful tool for non-contact and non-destructive determination of living cells properties. Here we use Raman tweezers to follow response of cells on the length of their cultivation in mineral oil. Analyses of obtained Raman spectra are based on 2D correlation analysis and allow us to determine the chemical background of the cell response in a gentle way.