D 2016

Time evolution of trapped single cell microorganism

BERNATOVÁ, Silvie, O SAMEK, S OBRUCA, M SERY, P ZEMANEK et. al.

Základní údaje

Originální název

Time evolution of trapped single cell microorganism

Autoři

BERNATOVÁ, Silvie, O SAMEK, S OBRUCA, M SERY, P ZEMANEK a I MAROVA

Vydání

BELLINGHAM, IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX, od s. "SPIE"-"3", 4 s. 2016

Nakladatel

SPIE-INT SOC OPTICAL ENGINEERING

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Utajení

není předmětem státního či obchodního tajemství

ISSN

UT WoS

000378222000001

Klíčová slova anglicky

Optical tweezers; Raman spectroscopy; 2D correlation analysis; external stimuli
Změněno: 29. 8. 2016 10:09, Mgr. Silvie Bernatová, Ph.D.

Anotace

V originále

The combination of optical tweezers and Raman micro-spectroscopy is frequently referred as Raman tweezers. A single focused laser beam is utilized here both as a source of Raman scattering and a source forming an optical trap. Raman tweezers have been recently used in variety of applications in cell biology as a useful tool for non-contact and non-destructive determination of living cells properties. Here we use Raman tweezers to follow response of cells on the length of their cultivation in mineral oil. Analyses of obtained Raman spectra are based on 2D correlation analysis and allow us to determine the chemical background of the cell response in a gentle way.