Gelatin-templated Nanostructured Gold for Electrochemical
Detection of Glucose in Serum

a 2016

Gelatin-templated Nanostructured Gold for Electrochemical Detection of Glucose in Serum

FARKA, Zdeněk, Tomáš JUŘÍK, Pavel PODEŠVA, David KOVÁŘ, František FORET et. al.

Základní údaje

Originální název

Gelatin-templated Nanostructured Gold for Electrochemical Detection of Glucose in Serum

Autoři

FARKA, Zdeněk, Tomáš JUŘÍK, Pavel PODEŠVA, David KOVÁŘ, František FORET a Petr SKLÁDAL

Vydání

67th Annual Meeting of the International Society of Electrochemistry, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10405 Electrochemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Gold nanostructures; Gelatin template; Glucose electrooxidation; Non-enzymatic sensor; Blood analysis

Příznaky

Mezinárodní význam

Změněno: 30. 8. 2016 15:48, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Nanostructured electrodes based on gelatin-templated gold were developed for sensitive non-enzymatic detection of glucose. The structures of specific saw tooth-like morphology were formed by electrodeposition of gold through the cavities of crosslinked gelatin. The growth of nanostructures was investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electroactive surface area (ESA) and electrochemical behavior of the prepared electrodes were determined by the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The roughness factor (expressed as ESA divided by geometrical area) of the gelatin-templated surface was estimated to be 16.7, which represent 15.9 fold enlargement compared to the flat gold electrode. The nanoscopic surface exhibited strong electrocatalytic effect towards glucose oxidation. The detection of glucose was done by direct electrochemical oxidation during CV and amperometry. In case of CV, the sensitivity of 1165 μA·mM-1·cm-2, wide linear range from 32 μM to 10 mM and limit of detection (LOD) of 90 μM were achieved. The amperometry allowed to further improve the LOD to 1.5 μM with very low applied potential of 0 V vs Ag. The detection of glucose in deproteinized human serum samples was possible with negligible interference of oxidizable blood components. The results were verified by a commercial glucometer and standard kit for photometric detection of glucose. Strong correlation between the methods was confirmed; the determination of glucose in serum was performed with a relative standard deviation of 4 %. The developed sensor exhibits a great potential for rapid and sensitive non-enzymatic glucose detection in clinical applications.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

FARKA, Zdeněk, Tomáš JUŘÍK, Pavel PODEŠVA, David KOVÁŘ, František FORET a Petr SKLÁDAL. Gelatin-templated Nanostructured Gold for Electrochemical Detection of Glucose in Serum. In 67th Annual Meeting of the International Society of Electrochemistry. 2016.

@proceedings{1352748,
   author = {Farka, Zdeněk and Juřík, Tomáš and Podešva, Pavel and Kovář, David and Foret, František and Skládal, Petr},
   booktitle = {67th Annual Meeting of the International Society of Electrochemistry},
   keywords = {Gold nanostructures; Gelatin template; Glucose electrooxidation; Non-enzymatic sensor; Blood analysis},
   language = {eng},
   title = {Gelatin-templated Nanostructured Gold for Electrochemical Detection of Glucose in Serum},
   url = {http://fpa.fontismedia.com/infiles/doc_production/ise160769.pdf},
   year = {2016}
}

TY  - CONF
ID  - 1352748
AU  - Farka, Zdeněk - Juřík, Tomáš - Podešva, Pavel - Kovář, David - Foret, František - Skládal, Petr
PY  - 2016
TI  - Gelatin-templated Nanostructured Gold for Electrochemical Detection of Glucose in Serum
KW  - Gold nanostructures
KW  - Gelatin template
KW  - Glucose electrooxidation
KW  - Non-enzymatic sensor
KW  - Blood analysis
UR  - http://fpa.fontismedia.com/infiles/doc_production/ise160769.pdf
N2  - Nanostructured electrodes based on gelatin-templated gold were developed for sensitive non-enzymatic detection of glucose. The structures of specific saw tooth-like morphology were formed by electrodeposition of gold through the cavities of crosslinked gelatin. The growth of nanostructures was investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electroactive surface area (ESA) and electrochemical behavior of the prepared electrodes were determined by the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The roughness factor (expressed as ESA divided by geometrical area) of the gelatin-templated surface was estimated to be 16.7, which represent 15.9 fold enlargement compared to the flat gold electrode. The nanoscopic surface exhibited strong electrocatalytic effect towards glucose oxidation. The detection of glucose was done by direct electrochemical oxidation during CV and amperometry. In case of CV, the sensitivity of 1165 μA·mM-1·cm-2, wide linear range from 32 μM to 10 mM and limit of detection (LOD) of 90 μM were achieved. The amperometry allowed to further improve the LOD to 1.5 μM with very low applied potential of 0 V vs Ag. The detection of glucose in deproteinized human serum samples was possible with negligible interference of oxidizable blood components. The results were verified by a commercial glucometer and standard kit for photometric detection of glucose. Strong correlation between the methods was confirmed; the determination of glucose in serum was performed with a relative standard deviation of 4 %. The developed sensor exhibits a great potential for rapid and sensitive non-enzymatic glucose detection in clinical applications.
ER  -

FARKA, Zdeněk, Tomáš JUŘÍK, Pavel PODEŠVA, David KOVÁŘ, František FORET a Petr SKLÁDAL. Gelatin-templated Nanostructured Gold for Electrochemical Detection of Glucose in Serum. In \textit{67th Annual Meeting of the International Society of Electrochemistry}. 2016.

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