| RIEDL, J, P MENOVA, R POHL, Petr ORSÁG, Miroslav FOJTA a Michal HOCEK. GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions. Journal of Organic Chemistry. WASHINGTON: AMER CHEMICAL SOC, 2012, roč. 77, č. 18, s. 8287-8293. ISSN 0022-3263. Dostupné z: https://dx.doi.org/10.1021/jo301684b. |
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@article{1353377,
author = {Riedl, J and Menova, P and Pohl, R and Orság, Petr and Fojta, Miroslav and Hocek, Michal},
article_location = {WASHINGTON},
article_number = {18},
doi = {http://dx.doi.org/10.1021/jo301684b},
language = {eng},
issn = {0022-3263},
journal = {Journal of Organic Chemistry},
title = {GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions},
volume = {77},
year = {2012}
}
TY - JOUR
ID - 1353377
AU - Riedl, J - Menova, P - Pohl, R - Orság, Petr - Fojta, Miroslav - Hocek, Michal
PY - 2012
TI - GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions
JF - Journal of Organic Chemistry
VL - 77
IS - 18
SP - 8287-8293
EP - 8287-8293
PB - AMER CHEMICAL SOC
SN - 00223263
N2 - GFP-like 3,5-difluoro-4-hydroxybenzylideneimidazolinone (FBI) and 3,5-bis(methoxy)-4-hydroxy-benzylideneimidazolinone (MBI) labels were attached to dCTP through a propargyl linker, and the resulting labeled nucleotides (dC(MBI)TP and dC(FBI)TP) were used for a facile enzymatic synthesis of oligonucleotide or DNA probes by polymerase-catalyzed primer extension. The MBI/FBI-labeled DNA probes exerted low fluorescence that was increased 2-3.2 times upon binding of a protein. The concept was demonstrated on sequence-specific binding of p53 to dsDNA and on nonspecific binding of single strand binding protein to an oligonucleotide. The FBI label was also used for a time-resolved experiment monitoring a single-nucleotide incorporation followed by primer extension by Vent(exo-) polymerase.
ER -
RIEDL, J, P MENOVA, R POHL, Petr ORSÁG, Miroslav FOJTA a Michal HOCEK. GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions. \textit{Journal of Organic Chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2012, roč.~77, č.~18, s.~8287-8293. ISSN~0022-3263. Dostupné z: https://dx.doi.org/10.1021/jo301684b.
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