2012
Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions
RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA et. al.Základní údaje
Originální název
Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions
Autoři
RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA a Michal HOCEK
Vydání
CHEMICAL SCIENCE, Cambridge, ROYAL SOC CHEMISTRY, 2012, 2041-6520
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 8.314
UT WoS
000306931300019
Změněno: 7. 9. 2016 11:00, Mgr. Petr Orság, Ph.D.
Anotace
V originále
Solvatochromic fluorescent 4-aminophthalimide (API) and 4-(N, N-dimethylamino) phthalimide (DAPI) were attached covalently to 2'-deoxycytidine or -adenosine via a non-conjugated propargyl linker by Sonogashira cross-coupling reactions of N-propargylphthalimides with halogenated nucleosides. The nucleosides were phosphorylated to triphosphates and enzymatically incorporated into oligonucleotides by DNA polymerases. API-labelled DNA was used for the detection of DNA protein interactions with either the sequence-specific p53 protein or a non-specific single strand binding (SSB) protein. Both proteins changed the polarity around the fluorophore and increased (2-3 fold) the intensity of API fluorescence.