Labelling of nucleosides and oligonucleotides by solvatochromic
4-aminophthalimide fluorophore for studying DNA-protein
interactions

J 2012

Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions

RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA et. al.

Základní údaje

Originální název

Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions

Autoři

RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA a Michal HOCEK

Vydání

CHEMICAL SCIENCE, Cambridge, ROYAL SOC CHEMISTRY, 2012, 2041-6520

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 8.314

DOI

http://dx.doi.org/10.1039/c2sc20404e

UT WoS

000306931300019

Změněno: 7. 9. 2016 11:00, Mgr. Petr Orság, Ph.D.

Anotace

V originále

Solvatochromic fluorescent 4-aminophthalimide (API) and 4-(N, N-dimethylamino) phthalimide (DAPI) were attached covalently to 2'-deoxycytidine or -adenosine via a non-conjugated propargyl linker by Sonogashira cross-coupling reactions of N-propargylphthalimides with halogenated nucleosides. The nucleosides were phosphorylated to triphosphates and enzymatically incorporated into oligonucleotides by DNA polymerases. API-labelled DNA was used for the detection of DNA protein interactions with either the sequence-specific p53 protein or a non-specific single strand binding (SSB) protein. Both proteins changed the polarity around the fluorophore and increased (2-3 fold) the intensity of API fluorescence.

Citovat

RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA a Michal HOCEK. Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions. CHEMICAL SCIENCE. Cambridge: ROYAL SOC CHEMISTRY, 2012, roč. 3, č. 9, s. 2797-2806. ISSN 2041-6520. Dostupné z: https://dx.doi.org/10.1039/c2sc20404e.

@article{1353378,
   author = {Riedl, J and Pohl, R and Ernsting, NP and Orság, Petr and Fojta, Miroslav and Hocek, Michal},
   article_location = {Cambridge},
   article_number = {9},
   doi = {http://dx.doi.org/10.1039/c2sc20404e},
   language = {eng},
   issn = {2041-6520},
   journal = {CHEMICAL SCIENCE},
   title = {Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions},
   volume = {3},
   year = {2012}
}

TY  - JOUR
ID  - 1353378
AU  - Riedl, J - Pohl, R - Ernsting, NP - Orság, Petr - Fojta, Miroslav - Hocek, Michal
PY  - 2012
TI  - Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions
JF  - CHEMICAL SCIENCE
VL  - 3
IS  - 9
SP  - 2797-2806
EP  - 2797-2806
PB  - ROYAL SOC CHEMISTRY
SN  - 20416520
N2  - Solvatochromic fluorescent 4-aminophthalimide (API) and 4-(N, N-dimethylamino) phthalimide (DAPI) were attached covalently to 2'-deoxycytidine or -adenosine via a non-conjugated propargyl linker by Sonogashira cross-coupling reactions of N-propargylphthalimides with halogenated nucleosides. The nucleosides were phosphorylated to triphosphates and enzymatically incorporated into oligonucleotides by DNA polymerases. API-labelled DNA was used for the detection of DNA protein interactions with either the sequence-specific p53 protein or a non-specific single strand binding (SSB) protein. Both proteins changed the polarity around the fluorophore and increased (2-3 fold) the intensity of API fluorescence.
ER  -

RIEDL, J, R POHL, NP ERNSTING, Petr ORSÁG, Miroslav FOJTA a Michal HOCEK. Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions. \textit{CHEMICAL SCIENCE}. Cambridge: ROYAL SOC CHEMISTRY, 2012, roč.~3, č.~9, s.~2797-2806. ISSN~2041-6520. Dostupné z: https://dx.doi.org/10.1039/c2sc20404e.

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