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@article{1354102,
author = {Fajkus, Petr and Peška, Vratislav and Šeděnka, Zdeňka and Fulnečková, Jana and Dvořáčková, Martina and Gogela, Roman and Sýkorová, Eva and Hapala, Jan and Fajkus, Jiří},
article_location = {Hoboken},
article_number = {3},
doi = {http://dx.doi.org/10.1111/tpj.13115},
keywords = {Allium; unusual telomere; telomerase; TERT; evolution; onion; genome sequencing},
language = {eng},
issn = {0960-7412},
journal = {The Plant Journal},
title = {Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase},
url = {http://onlinelibrary.wiley.com/doi/10.1111/tpj.13115/abstract;jsessionid=DD9460E772BBCE39FC663F17EDC51B3F.f02t03},
volume = {85},
year = {2016}
}
TY - JOUR
ID - 1354102
AU - Fajkus, Petr - Peška, Vratislav - Šeděnka, Zdeňka - Fulnečková, Jana - Dvořáčková, Martina - Gogela, Roman - Sýkorová, Eva - Hapala, Jan - Fajkus, Jiří
PY - 2016
TI - Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase
JF - The Plant Journal
VL - 85
IS - 3
SP - 337-347
EP - 337-347
PB - Wiley-Blackwell
SN - 09607412
KW - Allium
KW - unusual telomere
KW - telomerase
KW - TERT
KW - evolution
KW - onion
KW - genome sequencing
UR - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13115/abstract;jsessionid=DD9460E772BBCE39FC663F17EDC51B3F.f02t03
L2 - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13115/abstract;jsessionid=DD9460E772BBCE39FC663F17EDC51B3F.f02t03
N2 - Phylogenetic divergence in Asparagales plants is associated with switches in telomere sequences. The last switch occurred with divergence of the genus Allium (Amaryllidaceae) from the other Allioideae (formerly Alliaceae) genera, resulting in uncharacterized telomeres maintained by an unknown mechanism. To characterize the unknown Allium telomeres, we applied a combination of bioinformatic processing of transcriptomic and genomic data with standard approaches in telomere biology such as BAL31 sensitivity tests, terminal restriction fragment analysis, the telomere repeat amplification protocol (TRAP), and fluorescence insitu hybridization (FISH). Using these methods, we characterize the unusual telomeric sequence (CTCGGTTATGGG)(n) present in Allium species, demonstrate its synthesis by telomerase, and characterize the telomerase reverse transcriptase (TERT) subunit of Allium cepa. Our findings open up the possibility of studying the molecular details of the evolutionary genetic change in Allium telomeres and its possible role in speciation. Experimental studies addressing the implications of this change in terms of the interplay of telomere components may now be designed to shed more light on telomere functions and evolution in general.
ER -
FAJKUS, Petr, Vratislav PEŠKA, Zdeňka ŠEDĚNKA, Jana FULNEČKOVÁ, Martina DVOŘÁČKOVÁ, Roman GOGELA, Eva SÝKOROVÁ, Jan HAPALA a Jiří FAJKUS. Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase. \textit{The Plant Journal}. Hoboken: Wiley-Blackwell, 2016, roč.~85, č.~3, s.~337-347. ISSN~0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.13115.
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