Evaluation of anti-PAIIL lectin hen yolk antibody as an agent
inhibiting Pseudomonasaeruginosa adherence to epithelial cells

J 2016

Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells

VAŠKOVÁ, Lucie, Libuše NOSKOVÁ, Barbora BLÁHOVÁ, Michaela WIMMEROVÁ, Pavel DŘEVÍNEK et. al.

Základní údaje

Originální název

Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells

Autoři

VAŠKOVÁ, Lucie (203 Česká republika), Libuše NOSKOVÁ (203 Česká republika), Barbora BLÁHOVÁ (203 Česká republika), Michaela WIMMEROVÁ (203 Česká republika, domácí), Pavel DŘEVÍNEK (203 Česká republika), Božena KUBÍČKOVÁ (203 Česká republika), Marie STIBOROVÁ (203 Česká republika) a Petr HODEK (203 Česká republika, garant)

Vydání

Monatshefte für Chemie - Chemical Monthly, 2016, 0026-9247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.282

Kód RIV

RIV/00216224:14310/16:00090864

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s00706-016-1687-9

UT WoS

000374172200007

Klíčová slova anglicky

Receptors;Glycoproteins;Fluorescence spectroscopy;Cystic fibrosis;Antibacterial antibody

Štítky

AKR, cystic fibrosis, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 4. 2017 18:19, Ing. Andrea Mikešková

Anotace

V originále

Hen yolk antibody (IgY) against Pseudomonasaeruginosa (PA) virulence factor, lectin PAIIL, was prepared from two hens. As judged from ELISA, both animals produced equally reactive anti-PAIIL antibodies. The IgY prophylaxis against adhesion of PA on epithelium cell lines derived from normal or cystic fibrosis (CF) human lungs was examined. Both anti-PAIIL antibodies comparably prevented PA adherence on epithelial cells. In accordance with clinical data, cells from CF patient showed higher susceptibility to PA binding compared to cells of a healthy subject. In additional experiments, a luminescent PA strain (PA-lux) was used in the PA adhesion assay instead of an originally used regular PA strain (ST 1763). The employment of PA-lux in the assay is advantageous since the bacteria do not need to be fluorescently labeled for their quantification. The application of both non-luminescent PA (ST 1763) and newly tested PA-lux strains provides consistent results as far as the anti-PAIIL IgY protection against of PA adherence is concerned. Furthermore, in lung epithelium adherence assay we examined also other PA strains for their sensitivity to anti-PAIIL antibody. The adherence of all PA strains tested was reduced by anti-PAIIL antibody when compared with un-affected controls. This finding suggests a wide applicability of anti-PAIIL antibody in prophylaxis of PA lung infections.

Citovat

VAŠKOVÁ, Lucie, Libuše NOSKOVÁ, Barbora BLÁHOVÁ, Michaela WIMMEROVÁ, Pavel DŘEVÍNEK, Božena KUBÍČKOVÁ, Marie STIBOROVÁ a Petr HODEK. Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells. Monatshefte für Chemie - Chemical Monthly. 2016, roč. 147, č. 5, s. 889-896. ISSN 0026-9247. Dostupné z: https://dx.doi.org/10.1007/s00706-016-1687-9.

@article{1354565,
   author = {Vašková, Lucie and Nosková, Libuše and Bláhová, Barbora and Wimmerová, Michaela and Dřevínek, Pavel and Kubíčková, Božena and Stiborová, Marie and Hodek, Petr},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00706-016-1687-9},
   keywords = {Receptors;Glycoproteins;Fluorescence spectroscopy;Cystic fibrosis;Antibacterial antibody},
   language = {eng},
   issn = {0026-9247},
   journal = {Monatshefte für Chemie - Chemical Monthly},
   title = {Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells},
   volume = {147},
   year = {2016}
}

TY  - JOUR
ID  - 1354565
AU  - Vašková, Lucie - Nosková, Libuše - Bláhová, Barbora - Wimmerová, Michaela - Dřevínek, Pavel - Kubíčková, Božena - Stiborová, Marie - Hodek, Petr
PY  - 2016
TI  - Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells
JF  - Monatshefte für Chemie - Chemical Monthly
VL  - 147
IS  - 5
SP  - 889-896
EP  - 889-896
SN  - 00269247
KW  - Receptors;Glycoproteins;Fluorescence spectroscopy;Cystic fibrosis;Antibacterial antibody
N2  - Hen yolk antibody (IgY) against Pseudomonasaeruginosa (PA) virulence factor, lectin PAIIL, was prepared from two hens. As judged from ELISA, both animals produced equally reactive anti-PAIIL antibodies. The IgY prophylaxis against adhesion of PA on epithelium cell lines derived from normal or cystic fibrosis (CF) human lungs was examined. Both anti-PAIIL antibodies comparably prevented PA adherence on epithelial cells. In accordance with clinical data, cells from CF patient showed higher susceptibility to PA binding compared to cells of a healthy subject. In additional experiments, a luminescent PA strain (PA-lux) was used in the PA adhesion assay instead of an originally used regular PA strain (ST 1763). The employment of PA-lux in the assay is advantageous since the bacteria do not need to be fluorescently labeled for their quantification. The application of both non-luminescent PA (ST 1763) and newly tested PA-lux strains provides consistent results as far as the anti-PAIIL IgY protection against of PA adherence is concerned. Furthermore, in lung epithelium adherence assay we examined also other PA strains for their sensitivity to anti-PAIIL antibody. The adherence of all PA strains tested was reduced by anti-PAIIL antibody when compared with un-affected controls. This finding suggests a wide applicability of anti-PAIIL antibody in prophylaxis of PA lung infections.
ER  -

VAŠKOVÁ, Lucie, Libuše NOSKOVÁ, Barbora BLÁHOVÁ, Michaela WIMMEROVÁ, Pavel DŘEVÍNEK, Božena KUBÍČKOVÁ, Marie STIBOROVÁ a Petr HODEK. Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonasaeruginosa adherence to epithelial cells. \textit{Monatshefte für Chemie - Chemical Monthly}. 2016, roč.~147, č.~5, s.~889-896. ISSN~0026-9247. Dostupné z: https://dx.doi.org/10.1007/s00706-016-1687-9.

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