Real-time PCR versus digital PCR in diagnostics of food-borne
parasites

a 2016

Real-time PCR versus digital PCR in diagnostics of food-borne parasites

RESLOVÁ, Nikol; Martin KAŠNÝ; Michal SLANÝ; Silvia MONTEIRO; Ricardo SANTOS et. al.

Basic information

Original name

Real-time PCR versus digital PCR in diagnostics of food-borne parasites

Authors

RESLOVÁ, Nikol (203 Czech Republic, guarantor, belonging to the institution); Martin KAŠNÝ (203 Czech Republic, belonging to the institution); Michal SLANÝ (203 Czech Republic); Silvia MONTEIRO (620 Portugal) and Ricardo SANTOS (620 Portugal)

Edition

COST Action FA1408, EURO-FBP Meeting: “Analytical methods for foodborne parasites in human and veterinary diagnostics and in food matrices” 2016

Other information

Language

English

Type of outcome

Conference abstract

Field of Study

10600 1.6 Biological sciences

Country of publisher

Slovenia

Confidentiality degree

is not subject to a state or trade secret

References:

URL

RIV identification code

RIV/00216224:14310/16:00091069

Organization unit

Faculty of Science

ISBN

978-961-6956-48-2

Keywords (in Czech)

potravinoví parazité; bezpečnost potravin; diagnostika alimentárních infekcí; toxoplasma gondii; giardia intestinalis

Keywords in English

foodborne parasites; food safety; diagnosis of foodborne infections; toxoplasma gondii; giardia intestinalis

Abstract

In the original language

In recent years, an increased number of reported cases of human infections by parasitic agents present in foodstuffs is recorded in Europe. Among the dominant pathogens are documented protozoa - Giardia intestinalis and Toxoplasma gondii. Cysts of G. intestinalis can survive long term in a cool and moist environment and contaminate e.g. fruit and vegetables growing in soil. Whereas T. gondii is an obligate intracellular parasite of warm-blooded animals, which forms tissue cysts in nervous and muscle tissue of the intermediate host; during the meat processing might be contaminated also tissue of uninfected hosts. Moreover, meat inspection of T. gondii at the slaughterhouses is not legislatively established. The detection possibilities of G. intestinalis and T. gondii were tested using two molecular methods - quantitative real-time PCR (qPCR) and digital PCR (dPCR). While qPCR is a commonly used method for the detection of food-borne parasites, dPCR represents a new potentially fast, accurate and sensitive platform on this field. dPCR DNA chips enable to screen the absolute number of specifically amplified DNA molecules, which means that the high detection limit of pathogens could be reach. The aim of our study is to compare the efficiency of two methodological approaches adopted for detection of parasites in artificially contaminated samples of meat and vegetables by defined amounts of G. intestinalis (trofozoits) and T. gondii (oocysts).

Links

MUNI/A/1325/2015, interní kód MU

Name: Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí (Acronym: BIDA5)

Investor: Masaryk University, Category A

Cite

RESLOVÁ, Nikol; Martin KAŠNÝ; Michal SLANÝ; Silvia MONTEIRO and Ricardo SANTOS. Real-time PCR versus digital PCR in diagnostics of food-borne parasites. In COST Action FA1408, EURO-FBP Meeting: “Analytical methods for foodborne parasites in human and veterinary diagnostics and in food matrices”. 2016. ISBN 978-961-6956-48-2.

@proceedings{1355914,
   author = {Reslová, Nikol and Kašný, Martin and Slaný, Michal and Monteiro, Silvia and Santos, Ricardo},
   booktitle = {COST Action FA1408, EURO-FBP Meeting: “Analytical methods for foodborne parasites in human and veterinary diagnostics and in food matrices”},
   keywords = {foodborne parasites; food safety; diagnosis of foodborne infections; toxoplasma gondii; giardia intestinalis},
   language = {eng},
   isbn = {978-961-6956-48-2},
   title = {Real-time PCR versus digital PCR in diagnostics of food-borne parasites},
   url = {http://www.euro-fbp.org/meetings.html},
   year = {2016}
}

TY  - CONF
ID  - 1355914
AU  - Reslová, Nikol - Kašný, Martin - Slaný, Michal - Monteiro, Silvia - Santos, Ricardo
PY  - 2016
TI  - Real-time PCR versus digital PCR in diagnostics of food-borne parasites
SN  - 9789616956482
KW  - foodborne parasites
KW  - food safety
KW  - diagnosis of foodborne infections
KW  - toxoplasma gondii
KW  - giardia intestinalis
UR  - http://www.euro-fbp.org/meetings.html
N2  - In recent years, an increased number of reported cases of human infections by parasitic agents present in foodstuffs is recorded in Europe. Among the dominant pathogens are documented protozoa - Giardia intestinalis and Toxoplasma gondii. Cysts of G. intestinalis can survive long term in a cool and moist environment and contaminate e.g. fruit and vegetables growing in soil. Whereas T. gondii is an obligate intracellular parasite of warm-blooded animals, which forms tissue cysts in nervous and muscle tissue of the intermediate host; during the meat processing might be contaminated also tissue of uninfected hosts. Moreover, meat inspection of T. gondii at the slaughterhouses is not legislatively established. The detection possibilities of G. intestinalis and T. gondii were tested using two molecular methods - quantitative real-time PCR (qPCR) and digital PCR (dPCR). While qPCR is a commonly used method for the detection of food-borne parasites, dPCR represents a new potentially fast, accurate and sensitive platform on this field. dPCR DNA chips enable to screen the absolute number of specifically amplified DNA molecules, which means that the high detection limit of pathogens could be reach. The aim of our study is to compare the efficiency of two methodological approaches adopted for detection of parasites in artificially contaminated samples of meat and vegetables by defined amounts of G. intestinalis (trofozoits) and T. gondii (oocysts).
ER  -

RESLOVÁ, Nikol; Martin KAŠNÝ; Michal SLANÝ; Silvia MONTEIRO and Ricardo SANTOS. Real-time PCR versus digital PCR in diagnostics of food-borne parasites. In \textit{COST Action FA1408, EURO-FBP Meeting: “Analytical methods for foodborne parasites in human and veterinary diagnostics and in food matrices”}. 2016. ISBN~978-961-6956-48-2.

Přehled o publikaci