Detailed Information on Publication Record
2016
Insect immunity after invasion of entomopathogenic nematodes
HYRŠL, Pavel, Pavel DOBEŠ, Badrul AREFIN, Lucie KUČEROVÁ, Robert MARKUS et. al.Basic information
Original name
Insect immunity after invasion of entomopathogenic nematodes
Name in Czech
Insect immunity after invasion of entomopathogenic nematodes
Authors
HYRŠL, Pavel, Pavel DOBEŠ, Badrul AREFIN, Lucie KUČEROVÁ, Robert MARKUS, Wang ZHI, Michal ŽUROVEC and Ulrich THEOPOLD
Edition
32nd Symposium of European Society of Nematology, 2016
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
30102 Immunology
Country of publisher
Portugal
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Science
Keywords (in Czech)
imunita hmyzu; entomopatogenní hlístice
Keywords in English
insect immunity; entomopathogenic nematodes
Změněno: 9/10/2016 15:35, doc. RNDr. Pavel Hyršl, Ph.D.
V originále
Entomopathogenic nematodes and their associated bacteria comprise together a highly pathogenic complex which is able to invade and kill insect host within two days. Both bacteria and nematodes produce a variety of factors interacting with the insect immune system and help to overcome host defences. The tripartite model (Drosophila, nematodes, bacteria) was established and used to show an immune function for candidate genes using different Drosophila mutants or RNAi lines with defects in clotting or other branches of the immune system. Microarray analysis was used to compare gene expression of Drosophila larvae infected by the entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus luminescens with non-infected larvae. The role of candidate genes, selected based on genomic comparison, in response towards nematobacterial complex was further evaluated by in vivo infection assays. We demonstrated an immune function during nematode infection for known clotting enzymes and substrates, recognition molecules and eicosanoids. In conclusion, we show that the Heterorhabditis/Photorhabdus infection model is suitable to identify regulators of innate immunity in insects. Our research is supported by research grants from the Swedish Foundation for International Cooperation in Research and Higher Education (STINT), by grant from Ministry of Agriculture of Czech Republic (NAZV-KUS QJ1210047) and The Technology Agency of the Czech Republic (TA04020318).
In Czech
Entomopathogenic nematodes and their associated bacteria comprise together a highly pathogenic complex which is able to invade and kill insect host within two days. Both bacteria and nematodes produce a variety of factors interacting with the insect immune system and help to overcome host defences. The tripartite model (Drosophila, nematodes, bacteria) was established and used to show an immune function for candidate genes using different Drosophila mutants or RNAi lines with defects in clotting or other branches of the immune system. Microarray analysis was used to compare gene expression of Drosophila larvae infected by the entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus luminescens with non-infected larvae. The role of candidate genes, selected based on genomic comparison, in response towards nematobacterial complex was further evaluated by in vivo infection assays. We demonstrated an immune function during nematode infection for known clotting enzymes and substrates, recognition molecules and eicosanoids. In conclusion, we show that the Heterorhabditis/Photorhabdus infection model is suitable to identify regulators of innate immunity in insects. Our research is supported by research grants from the Swedish Foundation for International Cooperation in Research and Higher Education (STINT), by grant from Ministry of Agriculture of Czech Republic (NAZV-KUS QJ1210047) and The Technology Agency of the Czech Republic (TA04020318).
Links
| QJ1210047, research and development project |
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| TA04020318, research and development project |
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