Cystatins of the parasitic nematodes from the genus
Trichinella: serodiagnostic assays based on the
xMAP-technology.

k 2016

Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology.

ŠKORPÍKOVÁ, Lucie, Milan GELNAR, Michal SLANÝ, Martin KAŠNÝ, Petr KRÁLÍK et. al.

Basic information

Original name

Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology.

Name in Czech

Cystatiny parazitických hlístic rodu Trichinella: sérodiagnostické testy založené na xMAP technologii.

Authors

ŠKORPÍKOVÁ, Lucie (203 Czech Republic, guarantor, belonging to the institution), Milan GELNAR (203 Czech Republic), Michal SLANÝ (203 Czech Republic), Martin KAŠNÝ (203 Czech Republic), Petr KRÁLÍK (203 Czech Republic), Jana ILGOVÁ (703 Slovakia) and Karolína SNÍŽKOVÁ (203 Czech Republic)

Edition

EMOP XII – the 12th European Multicolloquium of Parasitology, 2016

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14310/16:00091133

Organization unit

Faculty of Science

Keywords (in Czech)

xMAP technologie; imunodiagnostika; cystatiny; Trichinella spiralis; Trichinella pseudospiralis

Keywords in English

xMAP technology; immunodiagnostics; cystatins; Trichinella spiralis; Trichinella pseudospiralis

Změněno: 24/3/2017 15:27, RNDr. Martin Kašný, Ph.D.

Abstract

V originále

Roundworms of the genus Trichinella are clinically important parasites invading cells of the small intestine and skeletal muscles. They infect a broad range of hosts involving birds, reptiles and mammals. Seven of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious human disease. During the life cycle, these parasites release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. They are essential for many biological processes like migration, digestion, development, and also in manipulation of the host immunne system. The dominant components of the E-S products are cystatins - reversible inhibitors of cysteine peptidases of C1 (papain-like) family and rarely of C13 (legumain-like) family. All the representatives of cystatin superfamily are evolutionary related and share some sequential and structural identity. Our work is focused on the characterization of "multi cystatin-like domain" (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared in Escherichia coli expression system. Currently, the recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnostics of Trichinella. We employed the xMAP technology - novel diagnostic platform based on magnetic microspheres and a signal evaluation by MAGPIX device.

Links

MUNI/A/1325/2015, interní kód MU

Name: Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí (Acronym: BIDA5)

Investor: Masaryk University, Category A

Citovat

ŠKORPÍKOVÁ, Lucie, Milan GELNAR, Michal SLANÝ, Martin KAŠNÝ, Petr KRÁLÍK, Jana ILGOVÁ and Karolína SNÍŽKOVÁ. Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology. In EMOP XII – the 12th European Multicolloquium of Parasitology. 2016.

@proceedings{1356195,
   author = {Škorpíková, Lucie and Gelnar, Milan and Slaný, Michal and Kašný, Martin and Králík, Petr and Ilgová, Jana and Snížková, Karolína},
   booktitle = {EMOP XII – the 12th European Multicolloquium of Parasitology},
   keywords = {xMAP technology; immunodiagnostics; cystatins; Trichinella spiralis; Trichinella pseudospiralis},
   language = {eng},
   title = {Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology.},
   url = {http://congress.utu.fi/emop2016/index.php},
   year = {2016}
}

TY  - CONF
ID  - 1356195
AU  - Škorpíková, Lucie - Gelnar, Milan - Slaný, Michal - Kašný, Martin - Králík, Petr - Ilgová, Jana - Snížková, Karolína
PY  - 2016
TI  - Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology.
KW  - xMAP technology
KW  - immunodiagnostics
KW  - cystatins
KW  - Trichinella spiralis
KW  - Trichinella pseudospiralis
UR  - http://congress.utu.fi/emop2016/index.php
N2  - Roundworms of the genus Trichinella are clinically important parasites invading cells of the small intestine and skeletal muscles. They infect a broad range of hosts involving birds, reptiles and mammals. Seven of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious human disease. During the life cycle, these parasites release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. They are essential for many biological processes like migration, digestion, development, and also in manipulation of the host immunne system. The dominant components of the E-S products are cystatins - reversible inhibitors of cysteine peptidases of C1 (papain-like) family and rarely of C13 (legumain-like) family. All the representatives of cystatin superfamily are evolutionary related and share some sequential and structural identity. Our work is focused on the characterization of "multi cystatin-like domain" (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared in Escherichia coli expression system. Currently, the recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnostics of Trichinella. We employed the xMAP technology - novel diagnostic platform based on magnetic microspheres and a signal evaluation by MAGPIX device.
ER  -

ŠKORPÍKOVÁ, Lucie, Milan GELNAR, Michal SLANÝ, Martin KAŠNÝ, Petr KRÁLÍK, Jana ILGOVÁ and Karolína SNÍŽKOVÁ. Cystatins of the parasitic nematodes from the genus Trichinella: serodiagnostic assays based on the xMAP-technology. In \textit{EMOP XII – the 12th European Multicolloquium of Parasitology}. 2016.

Detailed Information on Publication Record