Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer
cells to metabolic stress by disrupting actin cytoskeleton and
inhibiting autophagic flux

J 2016

Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux

PÁCHNIKOVÁ, Gabriela, Stjepan ULDRIJAN, Aleš IMRAMOVSKÝ, Vladimír KRYŠTOF, Iva SLANINOVÁ et. al.

Základní údaje

Originální název

Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux

Autoři

PÁCHNIKOVÁ, Gabriela (703 Slovensko, domácí), Stjepan ULDRIJAN (203 Česká republika, domácí), Aleš IMRAMOVSKÝ (203 Česká republika), Vladimír KRYŠTOF (203 Česká republika) a Iva SLANINOVÁ (203 Česká republika, garant, domácí)

Vydání

Toxicology in Vitro, Kidlington, Pergamon-Elsevier Science LTD, 2016, 0887-2333

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.866

Kód RIV

RIV/00216224:14110/16:00091211

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.tiv.2016.09.006

UT WoS

000387198300009

Klíčová slova anglicky

Actin; Autophagy; Melanoma; Metabolic stress; Sorafenib; Substituted 2-hydroxy-N-(arylalkyl)benzamid

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 1. 2017 12:39, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

N-((R)-1-(4-chlorophenylcarbamoyl)-2-phenylethyl)-5-chloro-2-hydroxybenzamide (Compound 6k), was recently isolated during the preparation of amino acids esters with salicylanilides. We show here that 6k disrupts the dynamics of actin cytoskeleton in human melanoma cells, affecting processes essential for the maintenance and expansion of tumours such as cell adhesion, motility, proliferation, vesicular transport, and autophagic flux. We demonstrated that inhibition of autophagy by 6k increased the sensitivity of melanoma cells to metabolic stress induced by rotenone or nutrient starvation and potentiated the anti-proliferative activity of small molecule multikinase inhibitor sorafenib. Since autophagy plays an important role in survival of cancer cells subjected to chemotherapy, the above mentioned properties are interesting from clinical point of view as 6k could promote metabolic stress within the tumour microenvironment and potentiate the effect of cytostatics in combination therapy.

Návaznosti

MUNI/A/1171/2015, interní kód MU

Název: Molekulárně buněčná biologie pro biomedicínu

Investor: Masarykova univerzita, Molekulárně buněčná biologie pro biomedicínu, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

PÁCHNIKOVÁ, Gabriela, Stjepan ULDRIJAN, Aleš IMRAMOVSKÝ, Vladimír KRYŠTOF a Iva SLANINOVÁ. Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux. Toxicology in Vitro. Kidlington: Pergamon-Elsevier Science LTD, 2016, roč. 37, "neuvedeno", s. 70-78. ISSN 0887-2333. Dostupné z: https://dx.doi.org/10.1016/j.tiv.2016.09.006.

@article{1356774,
   author = {Páchniková, Gabriela and Uldrijan, Stjepan and Imramovský, Aleš and Kryštof, Vladimír and Slaninová, Iva},
   article_location = {Kidlington},
   article_number = {"neuvedeno"},
   doi = {http://dx.doi.org/10.1016/j.tiv.2016.09.006},
   keywords = {Actin; Autophagy; Melanoma; Metabolic stress; Sorafenib; Substituted 2-hydroxy-N-(arylalkyl)benzamid},
   language = {eng},
   issn = {0887-2333},
   journal = {Toxicology in Vitro},
   title = {Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux},
   volume = {37},
   year = {2016}
}

TY  - JOUR
ID  - 1356774
AU  - Páchniková, Gabriela - Uldrijan, Stjepan - Imramovský, Aleš - Kryštof, Vladimír - Slaninová, Iva
PY  - 2016
TI  - Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux
JF  - Toxicology in Vitro
VL  - 37
IS  - "neuvedeno"
SP  - 70-78
EP  - 70-78
PB  - Pergamon-Elsevier Science LTD
SN  - 08872333
KW  - Actin
KW  - Autophagy
KW  - Melanoma
KW  - Metabolic stress
KW  - Sorafenib
KW  - Substituted 2-hydroxy-N-(arylalkyl)benzamid
N2  - N-((R)-1-(4-chlorophenylcarbamoyl)-2-phenylethyl)-5-chloro-2-hydroxybenzamide (Compound 6k), was recently isolated during the preparation of amino acids esters with salicylanilides. We show here that 6k disrupts the dynamics of actin cytoskeleton in human melanoma cells, affecting processes essential for the maintenance and expansion of tumours such as cell adhesion, motility, proliferation, vesicular transport, and autophagic flux. We demonstrated that inhibition of autophagy by 6k increased the sensitivity of melanoma cells to metabolic stress induced by rotenone or nutrient starvation and potentiated the anti-proliferative activity of small molecule multikinase inhibitor sorafenib. Since autophagy plays an important role in survival of cancer cells subjected to chemotherapy, the above mentioned properties are interesting from clinical point of view as 6k could promote metabolic stress within the tumour microenvironment and potentiate the effect of cytostatics in combination therapy.
ER  -

PÁCHNIKOVÁ, Gabriela, Stjepan ULDRIJAN, Aleš IMRAMOVSKÝ, Vladimír KRYŠTOF a Iva SLANINOVÁ. Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux. \textit{Toxicology in Vitro}. Kidlington: Pergamon-Elsevier Science LTD, 2016, roč.~37, ''neuvedeno'', s.~70-78. ISSN~0887-2333. Dostupné z: https://dx.doi.org/10.1016/j.tiv.2016.09.006.

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