Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer
cells to metabolic stress by disrupting actin cytoskeleton and
inhibiting autophagic flux

J 2016

Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux

PÁCHNIKOVÁ, Gabriela, Stjepan ULDRIJAN, Aleš IMRAMOVSKÝ, Vladimír KRYŠTOF, Iva SLANINOVÁ et. al.

Basic information

Original name

Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux

Authors

PÁCHNIKOVÁ, Gabriela (703 Slovakia, belonging to the institution), Stjepan ULDRIJAN (203 Czech Republic, belonging to the institution), Aleš IMRAMOVSKÝ (203 Czech Republic), Vladimír KRYŠTOF (203 Czech Republic) and Iva SLANINOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Toxicology in Vitro, Kidlington, Pergamon-Elsevier Science LTD, 2016, 0887-2333

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.866

RIV identification code

RIV/00216224:14110/16:00091211

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.tiv.2016.09.006

UT WoS

000387198300009

Keywords in English

Actin; Autophagy; Melanoma; Metabolic stress; Sorafenib; Substituted 2-hydroxy-N-(arylalkyl)benzamid

Abstract

V originále

N-((R)-1-(4-chlorophenylcarbamoyl)-2-phenylethyl)-5-chloro-2-hydroxybenzamide (Compound 6k), was recently isolated during the preparation of amino acids esters with salicylanilides. We show here that 6k disrupts the dynamics of actin cytoskeleton in human melanoma cells, affecting processes essential for the maintenance and expansion of tumours such as cell adhesion, motility, proliferation, vesicular transport, and autophagic flux. We demonstrated that inhibition of autophagy by 6k increased the sensitivity of melanoma cells to metabolic stress induced by rotenone or nutrient starvation and potentiated the anti-proliferative activity of small molecule multikinase inhibitor sorafenib. Since autophagy plays an important role in survival of cancer cells subjected to chemotherapy, the above mentioned properties are interesting from clinical point of view as 6k could promote metabolic stress within the tumour microenvironment and potentiate the effect of cytostatics in combination therapy.

Links

MUNI/A/1171/2015, interní kód MU

Name: Molekulárně buněčná biologie pro biomedicínu

Investor: Masaryk University, Category A

Citovat

PÁCHNIKOVÁ, Gabriela, Stjepan ULDRIJAN, Aleš IMRAMOVSKÝ, Vladimír KRYŠTOF and Iva SLANINOVÁ. Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux. Toxicology in Vitro. Kidlington: Pergamon-Elsevier Science LTD, 2016, vol. 37, "neuvedeno", p. 70-78. ISSN 0887-2333. Available from: https://dx.doi.org/10.1016/j.tiv.2016.09.006.

@article{1356774,
   author = {Páchniková, Gabriela and Uldrijan, Stjepan and Imramovský, Aleš and Kryštof, Vladimír and Slaninová, Iva},
   article_location = {Kidlington},
   article_number = {"neuvedeno"},
   doi = {http://dx.doi.org/10.1016/j.tiv.2016.09.006},
   keywords = {Actin; Autophagy; Melanoma; Metabolic stress; Sorafenib; Substituted 2-hydroxy-N-(arylalkyl)benzamid},
   language = {eng},
   issn = {0887-2333},
   journal = {Toxicology in Vitro},
   title = {Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux},
   volume = {37},
   year = {2016}
}

TY  - JOUR
ID  - 1356774
AU  - Páchniková, Gabriela - Uldrijan, Stjepan - Imramovský, Aleš - Kryštof, Vladimír - Slaninová, Iva
PY  - 2016
TI  - Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux
JF  - Toxicology in Vitro
VL  - 37
IS  - "neuvedeno"
SP  - 70-78
EP  - 70-78
PB  - Pergamon-Elsevier Science LTD
SN  - 08872333
KW  - Actin
KW  - Autophagy
KW  - Melanoma
KW  - Metabolic stress
KW  - Sorafenib
KW  - Substituted 2-hydroxy-N-(arylalkyl)benzamid
N2  - N-((R)-1-(4-chlorophenylcarbamoyl)-2-phenylethyl)-5-chloro-2-hydroxybenzamide (Compound 6k), was recently isolated during the preparation of amino acids esters with salicylanilides. We show here that 6k disrupts the dynamics of actin cytoskeleton in human melanoma cells, affecting processes essential for the maintenance and expansion of tumours such as cell adhesion, motility, proliferation, vesicular transport, and autophagic flux. We demonstrated that inhibition of autophagy by 6k increased the sensitivity of melanoma cells to metabolic stress induced by rotenone or nutrient starvation and potentiated the anti-proliferative activity of small molecule multikinase inhibitor sorafenib. Since autophagy plays an important role in survival of cancer cells subjected to chemotherapy, the above mentioned properties are interesting from clinical point of view as 6k could promote metabolic stress within the tumour microenvironment and potentiate the effect of cytostatics in combination therapy.
ER  -

PÁCHNIKOVÁ, Gabriela, Stjepan ULDRIJAN, Aleš IMRAMOVSKÝ, Vladimír KRYŠTOF and Iva SLANINOVÁ. Substituted 2-hydroxy-N-(arylalkyl)benzamide sensitizes cancer cells to metabolic stress by disrupting actin cytoskeleton and inhibiting autophagic flux. \textit{Toxicology in Vitro}. Kidlington: Pergamon-Elsevier Science LTD, 2016, vol.~37, ''neuvedeno'', p.~70-78. ISSN~0887-2333. Available from: https://dx.doi.org/10.1016/j.tiv.2016.09.006.

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