Colicins U and Y inhibit growth of Escherichia coli strains via
recognition of conserved OmpA extracellular loop 1

J 2016

Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1

BOSÁK, Juraj, Lenka MICENKOVÁ, Magda DOLEŽALOVÁ a David ŠMAJS

Základní údaje

Originální název

Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1

Autoři

BOSÁK, Juraj (703 Slovensko, domácí), Lenka MICENKOVÁ (703 Slovensko, domácí), Magda DOLEŽALOVÁ (203 Česká republika) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

International Journal of Medical Microbiology, Jena, Elsevier GMBH, 2016, 1438-4221

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.391

Kód RIV

RIV/00216224:14110/16:00091216

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.ijmm.2016.07.002

UT WoS

000387522200002

Klíčová slova anglicky

Colicin U; Colicin Y; Colicin-receptor interaction; Escherichia coli; OmpA

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 1. 2017 10:28, Soňa Böhmová

Anotace

V originále

Interactions of colicins U and Y with the OmpA (Outer membrane protein A) receptor molecule were studied using site-directed mutagenesis and colicin binding assay. A systematic mutagenesis of the colicin-susceptible OmpA sequence from Escherichia coli (OmpAEC) to the colicin-resistant OmpA sequence from Serratia marcescens (OmpASM) was performed in regions corresponding to extracellular OmpA loops 1-4. Susceptibility to colicins U and Y was significantly affected by the OmpA mutation in loop 1. As with functional analysis, a decrease in binding capacity of His-tagged colicin U was found for recombinant OmpA with a mutated segment in loop 1 compared to control OmpAEC. To verify the importance of the identified amino acid residues in OmpA loop 1, we introduced loop 1 from OmpAEC into OmpASM, which resulted in the substantial increase of susceptibility to colicins U and Y. In addition, colicins U and Y were tested against a panel of 118 bacteriocin non-producing strains of four Escherichia species, including E. coli (39 strains), E. fergusonii (10 strains), E. hermannii (42 strains), and E. vulneris (27 strains). A majority (82%) of E. coli strains was susceptible to colicins U and Y. Interestingly, colicins U and Y also inhibited all of the 30 tested multidrug-resistant E. coli O25b-ST131 isolates. These findings, together with the fact that OmpA loop 1 is important for bacterial virulence and is evolutionary conserved, offer the potential of using colicins U and Y as specific anti-OmpA loop 1 directed antibacterial proteins.

Návaznosti

MUNI/11/InGA04/2014, interní kód MU

Název: Alternativní antimikrobiální látky: studium vazby kolicinů na povrch patogenní bakterie

Investor: Masarykova univerzita, Alternativní antimikrobiální látky: studium vazby kolicinů na povrch patogenní bakterie

Citovat

BOSÁK, Juraj, Lenka MICENKOVÁ, Magda DOLEŽALOVÁ a David ŠMAJS. Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1. International Journal of Medical Microbiology. Jena: Elsevier GMBH, 2016, roč. 306, č. 7, s. 486-494. ISSN 1438-4221. Dostupné z: https://dx.doi.org/10.1016/j.ijmm.2016.07.002.

@article{1356794,
   author = {Bosák, Juraj and Micenková, Lenka and Doležalová, Magda and Šmajs, David},
   article_location = {Jena},
   article_number = {7},
   doi = {http://dx.doi.org/10.1016/j.ijmm.2016.07.002},
   keywords = {Colicin U; Colicin Y; Colicin-receptor interaction; Escherichia coli; OmpA},
   language = {eng},
   issn = {1438-4221},
   journal = {International Journal of Medical Microbiology},
   title = {Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1},
   volume = {306},
   year = {2016}
}

TY  - JOUR
ID  - 1356794
AU  - Bosák, Juraj - Micenková, Lenka - Doležalová, Magda - Šmajs, David
PY  - 2016
TI  - Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1
JF  - International Journal of Medical Microbiology
VL  - 306
IS  - 7
SP  - 486-494
EP  - 486-494
PB  - Elsevier GMBH
SN  - 14384221
KW  - Colicin U
KW  - Colicin Y
KW  - Colicin-receptor interaction
KW  - Escherichia coli
KW  - OmpA
N2  - Interactions of colicins U and Y with the OmpA (Outer membrane protein A) receptor molecule were studied using site-directed mutagenesis and colicin binding assay. A systematic mutagenesis of the colicin-susceptible OmpA sequence from Escherichia coli (OmpAEC) to the colicin-resistant OmpA sequence from Serratia marcescens (OmpASM) was performed in regions corresponding to extracellular OmpA loops 1-4. Susceptibility to colicins U and Y was significantly affected by the OmpA mutation in loop 1. As with functional analysis, a decrease in binding capacity of His-tagged colicin U was found for recombinant OmpA with a mutated segment in loop 1 compared to control OmpAEC. To verify the importance of the identified amino acid residues in OmpA loop 1, we introduced loop 1 from OmpAEC into OmpASM, which resulted in the substantial increase of susceptibility to colicins U and Y. In addition, colicins U and Y were tested against a panel of 118 bacteriocin non-producing strains of four Escherichia species, including E. coli (39 strains), E. fergusonii (10 strains), E. hermannii (42 strains), and E. vulneris (27 strains). A majority (82%) of E. coli strains was susceptible to colicins U and Y. Interestingly, colicins U and Y also inhibited all of the 30 tested multidrug-resistant E. coli O25b-ST131 isolates. These findings, together with the fact that OmpA loop 1 is important for bacterial virulence and is evolutionary conserved, offer the potential of using colicins U and Y as specific anti-OmpA loop 1 directed antibacterial proteins.
ER  -

BOSÁK, Juraj, Lenka MICENKOVÁ, Magda DOLEŽALOVÁ a David ŠMAJS. Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1. \textit{International Journal of Medical Microbiology}. Jena: Elsevier GMBH, 2016, roč.~306, č.~7, s.~486-494. ISSN~1438-4221. Dostupné z: https://dx.doi.org/10.1016/j.ijmm.2016.07.002.

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