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@article{1356794,
author = {Bosák, Juraj and Micenková, Lenka and Doležalová, Magda and Šmajs, David},
article_location = {Jena},
article_number = {7},
doi = {http://dx.doi.org/10.1016/j.ijmm.2016.07.002},
keywords = {Colicin U; Colicin Y; Colicin-receptor interaction; Escherichia coli; OmpA},
language = {eng},
issn = {1438-4221},
journal = {International Journal of Medical Microbiology},
title = {Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1},
volume = {306},
year = {2016}
}
TY - JOUR
ID - 1356794
AU - Bosák, Juraj - Micenková, Lenka - Doležalová, Magda - Šmajs, David
PY - 2016
TI - Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1
JF - International Journal of Medical Microbiology
VL - 306
IS - 7
SP - 486-494
EP - 486-494
PB - Elsevier GMBH
SN - 14384221
KW - Colicin U
KW - Colicin Y
KW - Colicin-receptor interaction
KW - Escherichia coli
KW - OmpA
N2 - Interactions of colicins U and Y with the OmpA (Outer membrane protein A) receptor molecule were studied using site-directed mutagenesis and colicin binding assay. A systematic mutagenesis of the colicin-susceptible OmpA sequence from Escherichia coli (OmpAEC) to the colicin-resistant OmpA sequence from Serratia marcescens (OmpASM) was performed in regions corresponding to extracellular OmpA loops 1-4. Susceptibility to colicins U and Y was significantly affected by the OmpA mutation in loop 1. As with functional analysis, a decrease in binding capacity of His-tagged colicin U was found for recombinant OmpA with a mutated segment in loop 1 compared to control OmpAEC. To verify the importance of the identified amino acid residues in OmpA loop 1, we introduced loop 1 from OmpAEC into OmpASM, which resulted in the substantial increase of susceptibility to colicins U and Y. In addition, colicins U and Y were tested against a panel of 118 bacteriocin non-producing strains of four Escherichia species, including E. coli (39 strains), E. fergusonii (10 strains), E. hermannii (42 strains), and E. vulneris (27 strains). A majority (82%) of E. coli strains was susceptible to colicins U and Y. Interestingly, colicins U and Y also inhibited all of the 30 tested multidrug-resistant E. coli O25b-ST131 isolates. These findings, together with the fact that OmpA loop 1 is important for bacterial virulence and is evolutionary conserved, offer the potential of using colicins U and Y as specific anti-OmpA loop 1 directed antibacterial proteins.
ER -
BOSÁK, Juraj, Lenka MICENKOVÁ, Magda DOLEŽALOVÁ a David ŠMAJS. Colicins U and Y inhibit growth of Escherichia coli strains via recognition of conserved OmpA extracellular loop 1. \textit{International Journal of Medical Microbiology}. Jena: Elsevier GMBH, 2016, roč.~306, č.~7, s.~486-494. ISSN~1438-4221. Dostupné z: https://dx.doi.org/10.1016/j.ijmm.2016.07.002.
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