Classification of Aeromonas spp. isolated from water and
clinical sources and distribution of virulence genes

J 2016

Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes

KRÁLOVÁ, Stanislava, Eva STAŇKOVÁ a Ivo SEDLÁČEK

Základní údaje

Originální název

Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes

Autoři

KRÁLOVÁ, Stanislava (703 Slovensko, garant, domácí), Eva STAŇKOVÁ (203 Česká republika, domácí) a Ivo SEDLÁČEK (203 Česká republika, domácí)

Vydání

Folia Microbiologica, 2016, 0015-5632

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.521

Kód RIV

RIV/00216224:14310/16:00091233

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s12223-016-0464-9

UT WoS

000386351700009

Klíčová slova anglicky

Aeromonas; virulence genes; virulence factors; identification; multiplex PCR;

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2018 23:29, prof. RNDr. Ivo Sedláček, CSc.

Anotace

V originále

In this work, 84 isolates of aeromonads were isolated from water and clinical samples, identified, and characterized. Identification was based on routine phenotyping combined with multiplex PCR. In this study, multiplex PCR was retested and reevaluated and its identification key was enhanced by 17 newly described species and five subspecies. Identification score increased from 36 % (only phenotyping) to 90% when supported with multiplex PCR. Further description of isolates included detection of eight virulence genes. These genes were overall present in 46 % (act), 2.4 % (ast), 80%(alt), 40%(ahh1), 20%(asa1), 69%(pla/lip/lipH3/alp- 1), 69 % (ser), and 81 % (fla), and no significant differences between water and clinical isolates were found. Results of this work show that the proper combination of different approaches is necessary for final identification of Aeromonas spp. at the species level. Multiplex PCR was shown to have limits in final identification, specifically inability to distinguish four species pairs and one triplet as their gene profiles are identical. However, it seems to be rapid and easy to do method able to support routine biochemical identification in laboratories. Moreover, our results supported previous proposal of reclassification of BAeromonas hydrophila subsp. dhakensis^ and BAeromonas aquariorum^ as identical species.

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

MUNI/A/0884/2013, interní kód MU

Název: Podpora výzkumných aktivit studentů mikrobiologie (Akronym: MIKROB)

Investor: Masarykova univerzita, Podpora výzkumných aktivit studentů mikrobiologie, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KRÁLOVÁ, Stanislava, Eva STAŇKOVÁ a Ivo SEDLÁČEK. Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes. Folia Microbiologica. 2016, roč. 61, č. 6, s. 513-521. ISSN 0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-016-0464-9.

@article{1356887,
   author = {Králová, Stanislava and Staňková, Eva and Sedláček, Ivo},
   article_number = {6},
   doi = {http://dx.doi.org/10.1007/s12223-016-0464-9},
   keywords = {Aeromonas; virulence genes; virulence factors; identification; multiplex PCR;},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes},
   volume = {61},
   year = {2016}
}

TY  - JOUR
ID  - 1356887
AU  - Králová, Stanislava - Staňková, Eva - Sedláček, Ivo
PY  - 2016
TI  - Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes
JF  - Folia Microbiologica
VL  - 61
IS  - 6
SP  - 513-521
EP  - 513-521
SN  - 00155632
KW  - Aeromonas
KW  - virulence genes
KW  - virulence factors
KW  - identification
KW  - multiplex PCR;
N2  - In this work, 84 isolates of aeromonads were isolated from water and clinical samples, identified, and characterized. Identification was based on routine phenotyping combined with multiplex PCR. In this study, multiplex PCR was retested and reevaluated and its identification key was enhanced by 17 newly described species and five subspecies. Identification score increased from 36 % (only phenotyping) to 90% when supported with multiplex PCR. Further description of isolates included detection of eight virulence genes. These genes were overall present in 46 % (act), 2.4 % (ast), 80%(alt), 40%(ahh1), 20%(asa1), 69%(pla/lip/lipH3/alp- 1), 69 % (ser), and 81 % (fla), and no significant differences between water and clinical isolates were found. Results of this work show that the proper combination of different approaches is necessary for final identification of Aeromonas spp. at the species level. Multiplex PCR was shown to have limits in final identification, specifically inability to distinguish four species pairs and one triplet as their gene profiles are identical. However, it seems to be rapid and easy to do method able to support routine biochemical identification in laboratories. Moreover, our results supported previous proposal of reclassification of BAeromonas hydrophila subsp. dhakensis^ and BAeromonas aquariorum^ as identical species.
ER  -

KRÁLOVÁ, Stanislava, Eva STAŇKOVÁ a Ivo SEDLÁČEK. Classification of Aeromonas spp. isolated from water and clinical sources and distribution of virulence genes. \textit{Folia Microbiologica}. 2016, roč.~61, č.~6, s.~513-521. ISSN~0015-5632. Dostupné z: https://dx.doi.org/10.1007/s12223-016-0464-9.

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