SUMOylation of Rad52-Rad59 synergistically change the outcome
of mitotic recombination

J 2016

SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination

SILVA, Sonia, Veronika ALTMANNOVÁ, Nadine ECKERT-BOULET, Peter KOLESÁR, Irene GALLINA et. al.

Základní údaje

Originální název

SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination

Autoři

SILVA, Sonia (208 Dánsko), Veronika ALTMANNOVÁ (203 Česká republika, domácí), Nadine ECKERT-BOULET (208 Dánsko), Peter KOLESÁR (703 Slovensko, domácí), Irene GALLINA (208 Dánsko), Lisa HANG (840 Spojené státy), Inn CHUNG (840 Spojené státy), Milica ARNERIC (840 Spojené státy), Xiaolan ZHAO (840 Spojené státy), Line Due BURON (208 Dánsko), Uffe H. MORTENSEN (208 Dánsko), Lumír KREJČÍ (203 Česká republika, garant, domácí) a Michael LISBY (208 Dánsko)

Vydání

DNA Repair, Amsterdam, Elsevier Science BV, 2016, 1568-7864

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.610

Kód RIV

RIV/00216224:14110/16:00088247

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.dnarep.2016.04.001

UT WoS

000377727400002

Klíčová slova anglicky

Homologous recombination; Rad52; Rad59; Srs2; Rad51; SUMOylation

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 11. 2016 16:16, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Homologous recombination (HR) is essential for maintenance of genome stability through double-strand break (DSB) repair, but at the same time HR can lead to loss of heterozygosity and uncontrolled recombination can be genotoxic. The post-translational modification by SUMO (small ubiquitin-like modifier) has been shown to modulate recombination, but the exact mechanism of this regulation remains unclear. Here we show that SUMOylation stabilizes the interaction between the recombination mediator Rad52 and its paralogue Rad59 in Saccharomyces cerevisiae. Although Rad59 SUMOylation is not required for survival after genotoxic stress, it affects the outcome of recombination to promote conservative DNA repair. In some genetic assays, Rad52 and Rad59 SUMOylation act synergistically. Collectively, our data indicate that the described SUMO modifications affect the balance between conservative and non-conservative mechanisms of HR. (C) 2016 Elsevier B.V. All rights reserved.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

ROZV/20/LF/2015, interní kód MU

Název: LF - Příspěvek IP 2015

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek IP 2015

Citovat

SILVA, Sonia, Veronika ALTMANNOVÁ, Nadine ECKERT-BOULET, Peter KOLESÁR, Irene GALLINA, Lisa HANG, Inn CHUNG, Milica ARNERIC, Xiaolan ZHAO, Line Due BURON, Uffe H. MORTENSEN, Lumír KREJČÍ a Michael LISBY. SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination. DNA Repair. Amsterdam: Elsevier Science BV, 2016, roč. 42, "neuvedeno", s. 11-25. ISSN 1568-7864. Dostupné z: https://dx.doi.org/10.1016/j.dnarep.2016.04.001.

@article{1357238,
   author = {Silva, Sonia and Altmannová, Veronika and EckertandBoulet, Nadine and Kolesár, Peter and Gallina, Irene and Hang, Lisa and Chung, Inn and Arneric, Milica and Zhao, Xiaolan and Buron, Line Due and Mortensen, Uffe H. and Krejčí, Lumír and Lisby, Michael},
   article_location = {Amsterdam},
   article_number = {"neuvedeno"},
   doi = {http://dx.doi.org/10.1016/j.dnarep.2016.04.001},
   keywords = {Homologous recombination; Rad52; Rad59; Srs2; Rad51; SUMOylation},
   language = {eng},
   issn = {1568-7864},
   journal = {DNA Repair},
   title = {SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination},
   volume = {42},
   year = {2016}
}

TY  - JOUR
ID  - 1357238
AU  - Silva, Sonia - Altmannová, Veronika - Eckert-Boulet, Nadine - Kolesár, Peter - Gallina, Irene - Hang, Lisa - Chung, Inn - Arneric, Milica - Zhao, Xiaolan - Buron, Line Due - Mortensen, Uffe H. - Krejčí, Lumír - Lisby, Michael
PY  - 2016
TI  - SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination
JF  - DNA Repair
VL  - 42
IS  - "neuvedeno"
SP  - 11-25
EP  - 11-25
PB  - Elsevier Science BV
SN  - 15687864
KW  - Homologous recombination
KW  - Rad52
KW  - Rad59
KW  - Srs2
KW  - Rad51
KW  - SUMOylation
N2  - Homologous recombination (HR) is essential for maintenance of genome stability through double-strand break (DSB) repair, but at the same time HR can lead to loss of heterozygosity and uncontrolled recombination can be genotoxic. The post-translational modification by SUMO (small ubiquitin-like modifier) has been shown to modulate recombination, but the exact mechanism of this regulation remains unclear. Here we show that SUMOylation stabilizes the interaction between the recombination mediator Rad52 and its paralogue Rad59 in Saccharomyces cerevisiae. Although Rad59 SUMOylation is not required for survival after genotoxic stress, it affects the outcome of recombination to promote conservative DNA repair. In some genetic assays, Rad52 and Rad59 SUMOylation act synergistically. Collectively, our data indicate that the described SUMO modifications affect the balance between conservative and non-conservative mechanisms of HR. (C) 2016 Elsevier B.V. All rights reserved.
ER  -

SILVA, Sonia, Veronika ALTMANNOVÁ, Nadine ECKERT-BOULET, Peter KOLESÁR, Irene GALLINA, Lisa HANG, Inn CHUNG, Milica ARNERIC, Xiaolan ZHAO, Line Due BURON, Uffe H. MORTENSEN, Lumír KREJČÍ a Michael LISBY. SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination. \textit{DNA Repair}. Amsterdam: Elsevier Science BV, 2016, roč.~42, ''neuvedeno'', s.~11-25. ISSN~1568-7864. Dostupné z: https://dx.doi.org/10.1016/j.dnarep.2016.04.001.

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