SUMOylation of Rad52-Rad59 synergistically change the outcome
of mitotic recombination

J 2016

SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination

SILVA, Sonia, Veronika ALTMANNOVÁ, Nadine ECKERT-BOULET, Peter KOLESÁR, Irene GALLINA et. al.

Basic information

Original name

SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination

Authors

SILVA, Sonia (208 Denmark), Veronika ALTMANNOVÁ (203 Czech Republic, belonging to the institution), Nadine ECKERT-BOULET (208 Denmark), Peter KOLESÁR (703 Slovakia, belonging to the institution), Irene GALLINA (208 Denmark), Lisa HANG (840 United States of America), Inn CHUNG (840 United States of America), Milica ARNERIC (840 United States of America), Xiaolan ZHAO (840 United States of America), Line Due BURON (208 Denmark), Uffe H. MORTENSEN (208 Denmark), Lumír KREJČÍ (203 Czech Republic, guarantor, belonging to the institution) and Michael LISBY (208 Denmark)

Edition

DNA Repair, Amsterdam, Elsevier Science BV, 2016, 1568-7864

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.610

RIV identification code

RIV/00216224:14110/16:00088247

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.dnarep.2016.04.001

UT WoS

000377727400002

Keywords in English

Homologous recombination; Rad52; Rad59; Srs2; Rad51; SUMOylation

Abstract

V originále

Homologous recombination (HR) is essential for maintenance of genome stability through double-strand break (DSB) repair, but at the same time HR can lead to loss of heterozygosity and uncontrolled recombination can be genotoxic. The post-translational modification by SUMO (small ubiquitin-like modifier) has been shown to modulate recombination, but the exact mechanism of this regulation remains unclear. Here we show that SUMOylation stabilizes the interaction between the recombination mediator Rad52 and its paralogue Rad59 in Saccharomyces cerevisiae. Although Rad59 SUMOylation is not required for survival after genotoxic stress, it affects the outcome of recombination to promote conservative DNA repair. In some genetic assays, Rad52 and Rad59 SUMOylation act synergistically. Collectively, our data indicate that the described SUMO modifications affect the balance between conservative and non-conservative mechanisms of HR. (C) 2016 Elsevier B.V. All rights reserved.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP207/12/2323, research and development project

Name: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Czech Science Foundation

GA13-26629S, research and development project

Name: SUMO a stability genomu

Investor: Czech Science Foundation

ROZV/20/LF/2015, interní kód MU

Name: LF - Příspěvek IP 2015

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SILVA, Sonia, Veronika ALTMANNOVÁ, Nadine ECKERT-BOULET, Peter KOLESÁR, Irene GALLINA, Lisa HANG, Inn CHUNG, Milica ARNERIC, Xiaolan ZHAO, Line Due BURON, Uffe H. MORTENSEN, Lumír KREJČÍ and Michael LISBY. SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination. DNA Repair. Amsterdam: Elsevier Science BV, 2016, vol. 42, "neuvedeno", p. 11-25. ISSN 1568-7864. Available from: https://dx.doi.org/10.1016/j.dnarep.2016.04.001.

@article{1357238,
   author = {Silva, Sonia and Altmannová, Veronika and EckertandBoulet, Nadine and Kolesár, Peter and Gallina, Irene and Hang, Lisa and Chung, Inn and Arneric, Milica and Zhao, Xiaolan and Buron, Line Due and Mortensen, Uffe H. and Krejčí, Lumír and Lisby, Michael},
   article_location = {Amsterdam},
   article_number = {"neuvedeno"},
   doi = {http://dx.doi.org/10.1016/j.dnarep.2016.04.001},
   keywords = {Homologous recombination; Rad52; Rad59; Srs2; Rad51; SUMOylation},
   language = {eng},
   issn = {1568-7864},
   journal = {DNA Repair},
   title = {SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination},
   volume = {42},
   year = {2016}
}

TY  - JOUR
ID  - 1357238
AU  - Silva, Sonia - Altmannová, Veronika - Eckert-Boulet, Nadine - Kolesár, Peter - Gallina, Irene - Hang, Lisa - Chung, Inn - Arneric, Milica - Zhao, Xiaolan - Buron, Line Due - Mortensen, Uffe H. - Krejčí, Lumír - Lisby, Michael
PY  - 2016
TI  - SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination
JF  - DNA Repair
VL  - 42
IS  - "neuvedeno"
SP  - 11-25
EP  - 11-25
PB  - Elsevier Science BV
SN  - 15687864
KW  - Homologous recombination
KW  - Rad52
KW  - Rad59
KW  - Srs2
KW  - Rad51
KW  - SUMOylation
N2  - Homologous recombination (HR) is essential for maintenance of genome stability through double-strand break (DSB) repair, but at the same time HR can lead to loss of heterozygosity and uncontrolled recombination can be genotoxic. The post-translational modification by SUMO (small ubiquitin-like modifier) has been shown to modulate recombination, but the exact mechanism of this regulation remains unclear. Here we show that SUMOylation stabilizes the interaction between the recombination mediator Rad52 and its paralogue Rad59 in Saccharomyces cerevisiae. Although Rad59 SUMOylation is not required for survival after genotoxic stress, it affects the outcome of recombination to promote conservative DNA repair. In some genetic assays, Rad52 and Rad59 SUMOylation act synergistically. Collectively, our data indicate that the described SUMO modifications affect the balance between conservative and non-conservative mechanisms of HR. (C) 2016 Elsevier B.V. All rights reserved.
ER  -

SILVA, Sonia, Veronika ALTMANNOVÁ, Nadine ECKERT-BOULET, Peter KOLESÁR, Irene GALLINA, Lisa HANG, Inn CHUNG, Milica ARNERIC, Xiaolan ZHAO, Line Due BURON, Uffe H. MORTENSEN, Lumír KREJČÍ and Michael LISBY. SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination. \textit{DNA Repair}. Amsterdam: Elsevier Science BV, 2016, vol.~42, ''neuvedeno'', p.~11-25. ISSN~1568-7864. Available from: https://dx.doi.org/10.1016/j.dnarep.2016.04.001.

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