MORN5 Expression during Craniofacial Development and Its
Interaction with the BMP and TGF beta Pathways

J 2016

MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGF beta Pathways

CELÁ, Petra, Marek HAMPL, Katherine K. FU, Michaela BOSÁKOVÁ, Pavel KREJČÍ et. al.

Základní údaje

Originální název

MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGF beta Pathways

Autoři

CELÁ, Petra (203 Česká republika, domácí), Marek HAMPL (203 Česká republika, domácí), Katherine K. FU (124 Kanada), Michaela BOSÁKOVÁ (203 Česká republika, domácí), Pavel KREJČÍ (203 Česká republika, domácí), Joy M. RICHMAN (124 Kanada) a marcela BUCHTOVÁ (203 Česká republika, garant, domácí)

Vydání

Frontiers in Physiology, Lausanne, FRONTIERS MEDIA SA, 2016, 1664-042X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.134

Kód RIV

RIV/00216224:14310/16:00094562

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3389/fphys.2016.00378

UT WoS

000382304700003

Klíčová slova anglicky

cleft lip; maxillary prominence; mandibular prominence; frontonasal mass; BMP

Štítky

EL OK, NZ, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 4. 2018 14:31, Ing. Nicole Zrilić

Anotace

V originále

MORN5 (MORN repeat containing 5) is encoded by a locus positioned on chromosome 17 in the chicken genome. The MORN motif is found in multiple copies in several proteins including junctophilins or phosphatidylinositol phosphate kinase family and the MORN proteins themselves are found across the animal and plant kingdoms. MORN5 protein has a characteristic punctate pattern in the cytoplasm in immunofluorescence imaging. Previously, MORN5 was found among differentially expressed genes in a microarray profiling experiment of the chicken embryo head. Here, we provided in situ hybridization to analyse, in detail, the MORN5 expression in chick craniofacial structures. The expression of MORN5 was first observed at stage HH17-18 (E2.5). MORN5 expression gradually appeared on either side of the primitive oral cavity, within the maxillary region. At stage HH20 (E3), prominent expression was localized in the mandibular prominences lateral to the midline. From stage HH20 up to HH29 (E6), there was strong expression in restricted regions of the maxillary and mandibular prominences. The frontonasal mass (in the midline of the face) expressed MORN5, starting at HH27 (E5). The expression was concentrated in the corners or globular processes, which will ultimately fuse with the cranial edges of the maxillary prominences. MORN5 expression was maintained in the fusion zone up to stage HH29. In sections MORN5 expression was localized preferentially in the mesenchyme. Previously, we examined signals that regulate MORN5 expression in the face based on a previous microarray study. Here, we validated the array results with in situ hybridization and QPCR. MORN5 was downregulated 24 h after Noggin and/or RA treatment. We also determined that BMP pathway genes are downstream of MORN5 following siRNA knockdown. Based on these results, we conclude that MORN5 is both regulated by and required for BMP signaling. The restricted expression of MORN5 in the lip fusion zone shown here supports the human genetic data in which MORN5 variants were associated with increased risk of non-syndromic cleft lip with or without cleft palate.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

NV15-33232A, projekt VaV

Název: Identifikace nových možností léčby achondroplásie prostřednictvím analýzy interakce FGFR3 a adaptérového proteinu Frs2

Citovat

CELÁ, Petra, Marek HAMPL, Katherine K. FU, Michaela BOSÁKOVÁ, Pavel KREJČÍ, Joy M. RICHMAN a marcela BUCHTOVÁ. MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGF beta Pathways. Frontiers in Physiology. Lausanne: FRONTIERS MEDIA SA, 2016, roč. 7, August, s. nestránkováno, 12 s. ISSN 1664-042X. Dostupné z: https://dx.doi.org/10.3389/fphys.2016.00378.

@article{1357383,
   author = {Celá, Petra and Hampl, Marek and Fu, Katherine K. and Bosáková, Michaela and Krejčí, Pavel and Richman, Joy M. and Buchtová, marcela},
   article_location = {Lausanne},
   article_number = {August},
   doi = {http://dx.doi.org/10.3389/fphys.2016.00378},
   keywords = {cleft lip; maxillary prominence; mandibular prominence; frontonasal mass; BMP},
   language = {eng},
   issn = {1664-042X},
   journal = {Frontiers in Physiology},
   title = {MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGF beta Pathways},
   volume = {7},
   year = {2016}
}

TY  - JOUR
ID  - 1357383
AU  - Celá, Petra - Hampl, Marek - Fu, Katherine K. - Bosáková, Michaela - Krejčí, Pavel - Richman, Joy M. - Buchtová, marcela
PY  - 2016
TI  - MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGF beta Pathways
JF  - Frontiers in Physiology
VL  - 7
IS  - August
SP  - nestránkováno
EP  - nestránkováno
PB  - FRONTIERS MEDIA SA
SN  - 1664042X
KW  - cleft lip
KW  - maxillary prominence
KW  - mandibular prominence
KW  - frontonasal mass
KW  - BMP
N2  - MORN5 (MORN repeat containing 5) is encoded by a locus positioned on chromosome 17 in the chicken genome. The MORN motif is found in multiple copies in several proteins including junctophilins or phosphatidylinositol phosphate kinase family and the MORN proteins themselves are found across the animal and plant kingdoms. MORN5 protein has a characteristic punctate pattern in the cytoplasm in immunofluorescence imaging. Previously, MORN5 was found among differentially expressed genes in a microarray profiling experiment of the chicken embryo head. Here, we provided in situ hybridization to analyse, in detail, the MORN5 expression in chick craniofacial structures. The expression of MORN5 was first observed at stage HH17-18 (E2.5). MORN5 expression gradually appeared on either side of the primitive oral cavity, within the maxillary region. At stage HH20 (E3), prominent expression was localized in the mandibular prominences lateral to the midline. From stage HH20 up to HH29 (E6), there was strong expression in restricted regions of the maxillary and mandibular prominences. The frontonasal mass (in the midline of the face) expressed MORN5, starting at HH27 (E5). The expression was concentrated in the corners or globular processes, which will ultimately fuse with the cranial edges of the maxillary prominences. MORN5 expression was maintained in the fusion zone up to stage HH29. In sections MORN5 expression was localized preferentially in the mesenchyme. Previously, we examined signals that regulate MORN5 expression in the face based on a previous microarray study. Here, we validated the array results with in situ hybridization and QPCR. MORN5 was downregulated 24 h after Noggin and/or RA treatment. We also determined that BMP pathway genes are downstream of MORN5 following siRNA knockdown. Based on these results, we conclude that MORN5 is both regulated by and required for BMP signaling. The restricted expression of MORN5 in the lip fusion zone shown here supports the human genetic data in which MORN5 variants were associated with increased risk of non-syndromic cleft lip with or without cleft palate.
ER  -

CELÁ, Petra, Marek HAMPL, Katherine K. FU, Michaela BOSÁKOVÁ, Pavel KREJČÍ, Joy M. RICHMAN a marcela BUCHTOVÁ. MORN5 Expression during Craniofacial Development and Its Interaction with the BMP and TGF beta Pathways. \textit{Frontiers in Physiology}. Lausanne: FRONTIERS MEDIA SA, 2016, roč.~7, August, s.~nestránkováno, 12 s. ISSN~1664-042X. Dostupné z: https://dx.doi.org/10.3389/fphys.2016.00378.

Podrobný výpis o publikaci