Microcin determinants are associated with B2 phylogroup of
human fecal Escherichia coli isolates

J 2016

Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates

MICENKOVÁ, Lenka, Juraj BOSÁK, Barbora ŠTAUDOVÁ, D. KOHOUTOVA, D. CEJKOVA et. al.

Základní údaje

Originální název

Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates

Autoři

MICENKOVÁ, Lenka (703 Slovensko, domácí), Juraj BOSÁK (703 Slovensko, domácí), Barbora ŠTAUDOVÁ (203 Česká republika, domácí), D. KOHOUTOVA (203 Česká republika), D. CEJKOVA (203 Česká republika), Vladana WOZNICOVÁ (203 Česká republika, domácí), M. VRBA (203 Česká republika), Alena ŠEVČÍKOVÁ (203 Česká republika, domácí), J. BURES (203 Česká republika) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

MicrobiologyOpen, Hoboken, Wiley-Blackwell, 2016, 2045-8827

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.747

Kód RIV

RIV/00216224:14110/16:00100694

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/mbo3.345

UT WoS

000378920700012

Klíčová slova anglicky

Bacteriocin; colicin; E; coli; microcin; phylogroup

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 11. 2018 15:05, Soňa Böhmová

Anotace

V originále

Escherichia coli strains are classified into four main phylogenetic groups (A, B1, B2, and D) and strains of these phylogroups differ in a number of characteristics. This study tested whether human fecal E. coli isolates belonging to different phylogroups differ in prevalence of bacteriocinogenic isolates and prevalence of individual bacteriocinogenic determinants. A set of 1283 fecal E. coli isolates from patients with different diseases was tested for the presence of DNA regions allowing classification into E. coli phylogroups and for the ability to produce bacteriocins (23 colicins and 7 microcins). Of the isolates tested, the most common was phylogroup B2 (38.3%) followed by phylogroups A (28.3%), D (26.3%) and B1 (7.2%). Altogether, 695 bacteriocin producers were identified representing 54.2% of all tested isolates. The highest prevalence of bacteriocin producers was found in group B2 (60.3%) and the lowest in group B1 (44.6%). Determinants encoding colicins E1, Ia, and microcin mV were most common in phylogroup A, determinants encoding microcins mM and mH47 were most common in phylogroup B2, and determinant encoding mB17 was most common in phylogroup D. The highest prevalence of bacteriocinogeny was found in phylogroup B2, suggesting that bacteriocinogeny and especially the synthesis of microcins was associated with virulent and resident E. coli strains.

Návaznosti

GA16-21649S, projekt VaV

Název: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Grantová agentura ČR, Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

NT13413, projekt VaV

Název: Stanovení apoptózy v biopticky odebraných vzorcích z tlustého střeva

Investor: Ministerstvo zdravotnictví ČR, Stanovení apoptózy v biopticky odebraných vzorcích z tlustého střeva

Citovat

MICENKOVÁ, Lenka, Juraj BOSÁK, Barbora ŠTAUDOVÁ, D. KOHOUTOVA, D. CEJKOVA, Vladana WOZNICOVÁ, M. VRBA, Alena ŠEVČÍKOVÁ, J. BURES a David ŠMAJS. Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates. MicrobiologyOpen. Hoboken: Wiley-Blackwell, 2016, roč. 5, č. 3, s. 490-498. ISSN 2045-8827. Dostupné z: https://dx.doi.org/10.1002/mbo3.345.

@article{1357485,
   author = {Micenková, Lenka and Bosák, Juraj and Štaudová, Barbora and Kohoutova, D. and Cejkova, D. and Woznicová, Vladana and Vrba, M. and Ševčíková, Alena and Bures, J. and Šmajs, David},
   article_location = {Hoboken},
   article_number = {3},
   doi = {http://dx.doi.org/10.1002/mbo3.345},
   keywords = {Bacteriocin; colicin; E; coli; microcin; phylogroup},
   language = {eng},
   issn = {2045-8827},
   journal = {MicrobiologyOpen},
   title = {Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates},
   volume = {5},
   year = {2016}
}

TY  - JOUR
ID  - 1357485
AU  - Micenková, Lenka - Bosák, Juraj - Štaudová, Barbora - Kohoutova, D. - Cejkova, D. - Woznicová, Vladana - Vrba, M. - Ševčíková, Alena - Bures, J. - Šmajs, David
PY  - 2016
TI  - Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates
JF  - MicrobiologyOpen
VL  - 5
IS  - 3
SP  - 490-498
EP  - 490-498
PB  - Wiley-Blackwell
SN  - 20458827
KW  - Bacteriocin
KW  - colicin
KW  - E
KW  - coli
KW  - microcin
KW  - phylogroup
N2  - Escherichia coli strains are classified into four main phylogenetic groups (A, B1, B2, and D) and strains of these phylogroups differ in a number of characteristics. This study tested whether human fecal E. coli isolates belonging to different phylogroups differ in prevalence of bacteriocinogenic isolates and prevalence of individual bacteriocinogenic determinants. A set of 1283 fecal E. coli isolates from patients with different diseases was tested for the presence of DNA regions allowing classification into E. coli phylogroups and for the ability to produce bacteriocins (23 colicins and 7 microcins). Of the isolates tested, the most common was phylogroup B2 (38.3%) followed by phylogroups A (28.3%), D (26.3%) and B1 (7.2%). Altogether, 695 bacteriocin producers were identified representing 54.2% of all tested isolates. The highest prevalence of bacteriocin producers was found in group B2 (60.3%) and the lowest in group B1 (44.6%). Determinants encoding colicins E1, Ia, and microcin mV were most common in phylogroup A, determinants encoding microcins mM and mH47 were most common in phylogroup B2, and determinant encoding mB17 was most common in phylogroup D. The highest prevalence of bacteriocinogeny was found in phylogroup B2, suggesting that bacteriocinogeny and especially the synthesis of microcins was associated with virulent and resident E. coli strains.
ER  -

MICENKOVÁ, Lenka, Juraj BOSÁK, Barbora ŠTAUDOVÁ, D. KOHOUTOVA, D. CEJKOVA, Vladana WOZNICOVÁ, M. VRBA, Alena ŠEVČÍKOVÁ, J. BURES a David ŠMAJS. Microcin determinants are associated with B2 phylogroup of human fecal Escherichia coli isolates. \textit{MicrobiologyOpen}. Hoboken: Wiley-Blackwell, 2016, roč.~5, č.~3, s.~490-498. ISSN~2045-8827. Dostupné z: https://dx.doi.org/10.1002/mbo3.345.

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