A new panel of proteins associated with metastasis in low-grade
breast cancer: a role in cell migration and invasiveness

a 2016

A new panel of proteins associated with metastasis in low-grade breast cancer: a role in cell migration and invasiveness

GALOCZOVÁ, Michaela, Josef MARYÁŠ, Petra KOVAŘÍKOVÁ, Lenka ČÁPKOVÁ, Jakub FAKTOR et. al.

Základní údaje

Originální název

A new panel of proteins associated with metastasis in low-grade breast cancer: a role in cell migration and invasiveness

Autoři

GALOCZOVÁ, Michaela, Josef MARYÁŠ, Petra KOVAŘÍKOVÁ, Lenka ČÁPKOVÁ, Jakub FAKTOR a Pavel BOUCHAL

Vydání

Goodbye Flat Biology: Models, Mechanisms and Microenvironment, Berlin, Germany, 2.- 5. 10. 2016, 2016

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Klíčová slova česky

rakovina prsu, buněčná invaze a migrace, 3D kultury

Klíčová slova anglicky

breast cancer, cell invassion and migration, 3D cultures

Příznaky

Mezinárodní význam

Změněno: 23. 1. 2017 15:39, doc. Mgr. Pavel Bouchal, Ph.D.

Anotace

V originále

Introduction: We recently identified a new panel of proteins associated with lymph-node metastasis of low-grade luminal A breast cancer using proteomics, transcriptomics and immunohistochemistry on the set of well characterized 96 tumors [Bouchal et al., Combined proteomics and transcriptomics identifies carboxypeptidase B1 and nuclear factor κB (NF-κB) associated proteins as putative biomarkers of metastasis in low grade breast cancer, Mol. Cell. Proteomics, 14(7), 1814-1830 (2015)]. Our subsequent research has focused on the role of these proteins in migration and invasiveness of cancer cells and on understanding their molecular role in these processes. Methods: The role of CPB1, PDLIM2, RNF25 and TRAF3IP2 proteins in migration and invasiveness of transiently transfected MCF7 breast cancer cells was studied using xCELLigence system (Roche) and Transwell assay (Corning). In parallel, SWATH proteomics analyses of corresponding whole proteome lysates of MCF7 cells collected 24, 48 and 72 h after the transfection were performed, complemented by pull-down-MS assays on protein-protein interactions. Results: Analyses of xCELLigence and Transwell data have confirmed statistically significant connection of increased levels of CPB1, PDLIM2, RNF25 and TRAF3IP2 with MCF7 cell migration and/or invasiveness. This work proceeds by studying migratory and invasive properties in 3D cultures. Analysis of proteome profiles and interaction network obtained by next-generation SWATH proteomics has revealed new potentially functionally interacting proteins associated with cell proliferation and adhesion. Acknowledgement: The work was supported by Czech Science Foundation (project No. 14-19250S), by the project MEYS - NPS I - LO1413 and by MH CZ - DRO (MMCI, 00209805). Conference participation was supported by the grant from Masaryk University (MUNI/A/1265/2015).

Návaznosti

GA14-19250S, projekt VaV

Název: Nový panel proteinů korelujících se stavem lymfatických uzlin u low-grade nádorů prsu: Klinická verifikace a úloha v invazivitě nádorových buněk

Investor: Grantová agentura ČR, Nový panel proteinů korelujících se stavem lymfatických uzlin u low-grade nádorů prsu: Klinická verifikace a úloha v invazivitě nádorových buněk

MUNI/A/1265/2015, interní kód MU

Název: Podpora biochemického výzkumu v roce 2016

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2016, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

GALOCZOVÁ, Michaela, Josef MARYÁŠ, Petra KOVAŘÍKOVÁ, Lenka ČÁPKOVÁ, Jakub FAKTOR a Pavel BOUCHAL. A new panel of proteins associated with metastasis in low-grade breast cancer: a role in cell migration and invasiveness. In Goodbye Flat Biology: Models, Mechanisms and Microenvironment, Berlin, Germany, 2.- 5. 10. 2016. 2016.

@proceedings{1358111,
   author = {Galoczová, Michaela and Maryáš, Josef and Kovaříková, Petra and Čápková, Lenka and Faktor, Jakub and Bouchal, Pavel},
   booktitle = {Goodbye Flat Biology: Models, Mechanisms and Microenvironment, Berlin, Germany, 2.- 5. 10. 2016},
   keywords = {breast cancer, cell invassion and migration, 3D cultures},
   title = {A new panel of proteins associated with metastasis in low-grade breast cancer: a role in cell migration and invasiveness},
   year = {2016}
}

TY  - CONF
ID  - 1358111
AU  - Galoczová, Michaela - Maryáš, Josef - Kovaříková, Petra - Čápková, Lenka - Faktor, Jakub - Bouchal, Pavel
PY  - 2016
TI  - A new panel of proteins associated with metastasis in low-grade breast cancer: a role in cell migration and invasiveness
KW  - breast cancer, cell invassion and migration, 3D cultures
N2  - Introduction: We recently identified a new panel of proteins associated with lymph-node metastasis of low-grade luminal A breast cancer using proteomics, transcriptomics and immunohistochemistry on the set of well characterized 96 tumors [Bouchal et al., Combined proteomics and transcriptomics identifies carboxypeptidase B1 and nuclear factor κB (NF-κB) associated proteins as putative biomarkers of metastasis in low grade breast cancer, Mol. Cell. Proteomics, 14(7), 1814-1830 (2015)]. Our subsequent research has focused on the role of these proteins in migration and invasiveness of cancer cells and on understanding their molecular role in these processes. Methods: The role of CPB1, PDLIM2, RNF25 and TRAF3IP2 proteins in migration and invasiveness of transiently transfected MCF7 breast cancer cells was studied using xCELLigence system (Roche) and Transwell assay (Corning). In parallel, SWATH proteomics analyses of corresponding whole proteome lysates of MCF7 cells collected 24, 48 and 72 h after the transfection were performed, complemented by pull-down-MS assays on protein-protein interactions. Results: Analyses of xCELLigence and Transwell data have confirmed statistically significant connection of increased levels of CPB1, PDLIM2, RNF25 and TRAF3IP2 with MCF7 cell migration and/or invasiveness. This work proceeds by studying migratory and invasive properties in 3D cultures. Analysis of proteome profiles and interaction network obtained by next-generation SWATH proteomics has revealed new potentially functionally interacting proteins associated with cell proliferation and adhesion. Acknowledgement: The work was supported by Czech Science Foundation (project No. 14-19250S), by the project MEYS - NPS I - LO1413 and by MH CZ - DRO (MMCI, 00209805). Conference participation was supported by the grant from Masaryk University (MUNI/A/1265/2015).
ER  -

GALOCZOVÁ, Michaela, Josef MARYÁŠ, Petra KOVAŘÍKOVÁ, Lenka ČÁPKOVÁ, Jakub FAKTOR a Pavel BOUCHAL. A new panel of proteins associated with metastasis in low-grade breast cancer: a role in cell migration and invasiveness. In \textit{Goodbye Flat Biology: Models, Mechanisms and Microenvironment, Berlin, Germany, 2.- 5. 10. 2016}. 2016.

Podrobný výpis o publikaci