Identification of miRNA expression profiles in human mesial
temporal lobe epilepsy with hippocampal sclerosis

a 2016

Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis

BENCÚROVÁ, Petra, Jiří BALOUN, Kateřina MUSILOVÁ, Karol PÁL, Nikola TOM et. al.

Základní údaje

Originální název

Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis

Název česky

Identifikace miRNA profilů v lidské meziotemporální epilepsií s hipokampální sklerózou

Autoři

BENCÚROVÁ, Petra (703 Slovensko, domácí), Jiří BALOUN (203 Česká republika, domácí), Kateřina MUSILOVÁ (203 Česká republika, domácí), Karol PÁL (203 Česká republika, domácí), Nikola TOM (203 Česká republika, domácí), Jakub HYNŠT (203 Česká republika, domácí), Lenka RADOVÁ (203 Česká republika, domácí), Martin PAIL (203 Česká republika, domácí), Eva BRICHTOVÁ (203 Česká republika), Markéta HERMANOVÁ (203 Česká republika, domácí), Šárka POSPÍŠILOVÁ (203 Česká republika, domácí), Marek MRÁZ (203 Česká republika, domácí) a Milan BRÁZDIL (203 Česká republika, garant, domácí)

Vydání

12th European Congress on Epileptology, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/16:00088294

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

miRNA; epilepsie; hipokampální skleróza; sekvenování; miQPCR

Klíčová slova anglicky

miRNA; epilepsy; hippocampal sclerosis; sequencing; miQPCR

Příznaky

Mezinárodní význam

Změněno: 23. 11. 2016 15:58, Mgr. Petra Bencúrová, Ph.D.

Anotace

V originále

Purpose: The primary aim of this study was to identify specific miRNAs with aberrant expression in hippocampal tissue of mesial temporal lobe epilepsy (mTLE) patients with hippocampal sclerosis (HS). Method: We analyzed miRNA isolated from paraffin embedded tissue slices of hippocampal complex from 22 mTLE +HS patients (14 left-sided, 8 right-sided) and 9 post mortem controls. To characterize the miRNAs highly prone to be involved in the pathological changes in the mTLE+HS brain tissue we performed the expression analysis combining the next generation sequencing (NGS) and novel specific technique for miRNA quantification miQPCR. Results: By both methodical approaches we confirmed significant upregulation of miR-129-1-3p, 129-2-3p, 142-3p, 191-5p, 193b-3p, 195-5p, 374b-5p, 451a, and 490-3p in mTLE+HS patients. The levels of miRNA-184 were significantly higher in control subjects. Differential expression levels in mTLE+ HS patients were identified also in the case of 10 miRNA species never considered to play a role in epilepsy before. Conclusion: Analytical approach combining NGS with miQPCR used for the first time for analysis of human epileptic foci led to reliable identification of miRNA with altered expression in mTLE +HS patients.

Návaznosti

GA16-04726S, projekt VaV

Název: miRNA a biochemické dráhy v epilepsii

Investor: Grantová agentura ČR, miRNA a biochemické dráhy v epilepsii

MUNI/A/1044/2015, interní kód MU

Název: miRNA jako biomarker meziotemporální epilepsie (Akronym: microRNA jako biomarker MTLE)

Investor: Masarykova univerzita, miRNA jako biomarker meziotemporální epilepsie, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

BENCÚROVÁ, Petra, Jiří BALOUN, Kateřina MUSILOVÁ, Karol PÁL, Nikola TOM, Jakub HYNŠT, Lenka RADOVÁ, Martin PAIL, Eva BRICHTOVÁ, Markéta HERMANOVÁ, Šárka POSPÍŠILOVÁ, Marek MRÁZ a Milan BRÁZDIL. Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis. In 12th European Congress on Epileptology. 2016.

@proceedings{1359053,
   author = {Bencúrová, Petra and Baloun, Jiří and Musilová, Kateřina and Pál, Karol and Tom, Nikola and Hynšt, Jakub and Radová, Lenka and Pail, Martin and Brichtová, Eva and Hermanová, Markéta and Pospíšilová, Šárka and Mráz, Marek and Brázdil, Milan},
   booktitle = {12th European Congress on Epileptology},
   keywords = {miRNA; epilepsy; hippocampal sclerosis; sequencing; miQPCR},
   language = {eng},
   title = {Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis},
   url = {http://www.epilepsyprague2016.org/},
   year = {2016}
}

TY  - CONF
ID  - 1359053
AU  - Bencúrová, Petra - Baloun, Jiří - Musilová, Kateřina - Pál, Karol - Tom, Nikola - Hynšt, Jakub - Radová, Lenka - Pail, Martin - Brichtová, Eva - Hermanová, Markéta - Pospíšilová, Šárka - Mráz, Marek - Brázdil, Milan
PY  - 2016
TI  - Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis
KW  - miRNA
KW  - epilepsy
KW  - hippocampal sclerosis
KW  - sequencing
KW  - miQPCR
UR  - http://www.epilepsyprague2016.org/
L2  - http://www.epilepsyprague2016.org/
N2  - Purpose: The primary aim of this study was to identify specific miRNAs with aberrant expression in hippocampal tissue of mesial temporal lobe epilepsy (mTLE) patients with hippocampal sclerosis (HS). Method: We analyzed miRNA isolated from paraffin embedded tissue slices of hippocampal complex from 22 mTLE +HS patients (14 left-sided, 8 right-sided) and 9 post mortem controls. To characterize the miRNAs highly prone to be involved in the pathological changes in the mTLE+HS brain tissue we performed the expression analysis combining the next generation sequencing (NGS) and novel specific technique for miRNA quantification miQPCR. Results: By both methodical approaches we confirmed significant upregulation of miR-129-1-3p, 129-2-3p, 142-3p, 191-5p, 193b-3p, 195-5p, 374b-5p, 451a, and 490-3p in mTLE+HS patients. The levels of miRNA-184 were significantly higher in control subjects. Differential expression levels in mTLE+ HS patients were identified also in the case of 10 miRNA species never considered to play a role in epilepsy before. Conclusion: Analytical approach combining NGS with miQPCR used for the first time for analysis of human epileptic foci led to reliable identification of miRNA with altered expression in mTLE +HS patients.
ER  -

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