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@proceedings{1359053,
author = {Bencúrová, Petra and Baloun, Jiří and Musilová, Kateřina and Pál, Karol and Tom, Nikola and Hynšt, Jakub and Radová, Lenka and Pail, Martin and Brichtová, Eva and Hermanová, Markéta and Pospíšilová, Šárka and Mráz, Marek and Brázdil, Milan},
booktitle = {12th European Congress on Epileptology},
keywords = {miRNA; epilepsy; hippocampal sclerosis; sequencing; miQPCR},
language = {eng},
title = {Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis},
url = {http://www.epilepsyprague2016.org/},
year = {2016}
}
TY - CONF
ID - 1359053
AU - Bencúrová, Petra - Baloun, Jiří - Musilová, Kateřina - Pál, Karol - Tom, Nikola - Hynšt, Jakub - Radová, Lenka - Pail, Martin - Brichtová, Eva - Hermanová, Markéta - Pospíšilová, Šárka - Mráz, Marek - Brázdil, Milan
PY - 2016
TI - Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis
KW - miRNA
KW - epilepsy
KW - hippocampal sclerosis
KW - sequencing
KW - miQPCR
UR - http://www.epilepsyprague2016.org/
L2 - http://www.epilepsyprague2016.org/
N2 - Purpose: The primary aim of this study was to identify specific miRNAs with aberrant expression in hippocampal tissue of mesial temporal lobe epilepsy (mTLE) patients with hippocampal sclerosis (HS). Method: We analyzed miRNA isolated from paraffin embedded tissue slices of hippocampal complex from 22 mTLE +HS patients (14 left-sided, 8 right-sided) and 9 post mortem controls. To characterize the miRNAs highly prone to be involved in the pathological changes in the mTLE+HS brain tissue we performed the expression analysis combining the next generation sequencing (NGS) and novel specific technique for miRNA quantification miQPCR. Results: By both methodical approaches we confirmed significant upregulation of miR-129-1-3p, 129-2-3p, 142-3p, 191-5p, 193b-3p, 195-5p, 374b-5p, 451a, and 490-3p in mTLE+HS patients. The levels of miRNA-184 were significantly higher in control subjects. Differential expression levels in mTLE+ HS patients were identified also in the case of 10 miRNA species never considered to play a role in epilepsy before. Conclusion: Analytical approach combining NGS with miQPCR used for the first time for analysis of human epileptic foci led to reliable identification of miRNA with altered expression in mTLE +HS patients.
ER -
BENCÚROVÁ, Petra, Jiří BALOUN, Kateřina MUSILOVÁ, Karol PÁL, Nikola TOM, Jakub HYNŠT, Lenka RADOVÁ, Martin PAIL, Eva BRICHTOVÁ, Markéta HERMANOVÁ, Šárka POSPÍŠILOVÁ, Marek MRÁZ a Milan BRÁZDIL. Identification of miRNA expression profiles in human mesial temporal lobe epilepsy with hippocampal sclerosis. In \textit{12th European Congress on Epileptology}. 2016.
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