2016
Multiple hypernation of LC with ESI MS, MALDI MS and SALD ICP MS - the detection platform for metalloproteomic studies
COUFALÍKOVÁ, Kateřina; Iva BENEŠOVÁ; Kristýna DLABKOVÁ; Viktor KANICKÝ; Jan PREISLER et al.Basic information
Original name
Multiple hypernation of LC with ESI MS, MALDI MS and SALD ICP MS - the detection platform for metalloproteomic studies
Name in Czech
ESI, MALDI a SALD ICP MS - detekční platforma pro metaloproteomická studia
Authors
Edition
Brno, Czech Republic, CECE 2016 13th International Interdisciplinary meeting on bioanalysis, p. 102-103, 2 pp. 2016
Publisher
Institute of Analytical Chemistry of the CAS, v.v. i., Brno, Czech Republic
Other information
Language
English
Type of outcome
Proceedings paper
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
Publication form
electronic version available online
Marked to be transferred to RIV
Yes
RIV identification code
RIV/00216224:14310/16:00088332
Organization unit
Faculty of Science
ISBN
978-80-904959-4-4
Keywords (in Czech)
MALDI MS; ESI MS; SALD ICP MS; metalothioneiny
Keywords in English
MALDI MS; ESI MS; SALD ICP MS; metallothioneins
Changed: 6/1/2017 13:13, RNDr. Kateřina Coufalíková, Ph.D.
In the original language
We present a new methodology for coupling conventional on-line LC-ESI MS with two off-line detection techniques: matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) analyses. The Off-line approach is facilitated using a commercial MALDI target made of conductive plastics, which is suitable for the both MALDI MS and SALD ICP MS. Due to this arrangement, two types of analysis, molecular and elemental determination of metallothionein 1 (MT-1) were performed.
In Czech
Byla prezentována nová metodologie spojení konvenčního on-line LC-ESI MS s dvěma off-line detekčními technikami: matrix-assisted laser desorption/ionization (MALDI) a substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS). Off-line přístup je umožněn použitím komerčního MALDI plastového terčíku zhotoveného z vodivého plastu, který je vhodný pro obě techniky: MALDI MS a SALD ICP MS. Díky tomuto uspořádání jsou umožněny současně dvě techniky, molekulární a elementární stanovení metalothioneinu 1 (MT-1).
Links
| GA15-05387S, research and development project |
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| LQ1601, research and development project |
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