D 2016

Multiple hypernation of LC with ESI MS, MALDI MS and SALD ICP MS - the detection platform for metalloproteomic studies

COUFALÍKOVÁ, Kateřina; Iva BENEŠOVÁ; Kristýna DLABKOVÁ; Viktor KANICKÝ; Jan PREISLER et al.

Basic information

Original name

Multiple hypernation of LC with ESI MS, MALDI MS and SALD ICP MS - the detection platform for metalloproteomic studies

Name in Czech

ESI, MALDI a SALD ICP MS - detekční platforma pro metaloproteomická studia

Authors

COUFALÍKOVÁ, Kateřina; Iva BENEŠOVÁ; Kristýna DLABKOVÁ; Viktor KANICKÝ and Jan PREISLER

Edition

Brno, Czech Republic, CECE 2016 13th International Interdisciplinary meeting on bioanalysis, p. 102-103, 2 pp. 2016

Publisher

Institute of Analytical Chemistry of the CAS, v.v. i., Brno, Czech Republic

Other information

Language

English

Type of outcome

Proceedings paper

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

Publication form

electronic version available online

Marked to be transferred to RIV

Yes

RIV identification code

RIV/00216224:14310/16:00088332

Organization unit

Faculty of Science

ISBN

978-80-904959-4-4

Keywords (in Czech)

MALDI MS; ESI MS; SALD ICP MS; metalothioneiny

Keywords in English

MALDI MS; ESI MS; SALD ICP MS; metallothioneins
Changed: 6/1/2017 13:13, RNDr. Kateřina Coufalíková, Ph.D.

Abstract

In the original language

We present a new methodology for coupling conventional on-line LC-ESI MS with two off-line detection techniques: matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) analyses. The Off-line approach is facilitated using a commercial MALDI target made of conductive plastics, which is suitable for the both MALDI MS and SALD ICP MS. Due to this arrangement, two types of analysis, molecular and elemental determination of metallothionein 1 (MT-1) were performed.

In Czech

Byla prezentována nová metodologie spojení konvenčního on-line LC-ESI MS s dvěma off-line detekčními technikami: matrix-assisted laser desorption/ionization (MALDI) a substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS). Off-line přístup je umožněn použitím komerčního MALDI plastového terčíku zhotoveného z vodivého plastu, který je vhodný pro obě techniky: MALDI MS a SALD ICP MS. Díky tomuto uspořádání jsou umožněny současně dvě techniky, molekulární a elementární stanovení metalothioneinu 1 (MT-1).

Links

GA15-05387S, research and development project
Name: Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic
Investor: Czech Science Foundation
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR