Prevalence of Propionibacterium acnes in Intervertebral Discs
of Patients Undergoing Lumbar Microdiscectomy: A Prospective
Cross-Sectional Study

J 2016

Prevalence of Propionibacterium acnes in Intervertebral Discs of Patients Undergoing Lumbar Microdiscectomy: A Prospective Cross-Sectional Study

CAPOOR, Manu N., Filip RŮŽIČKA, Táňa MACHÁČKOVÁ, Radim JANČÁLEK, Martin SMRČKA et. al.

Základní údaje

Originální název

Prevalence of Propionibacterium acnes in Intervertebral Discs of Patients Undergoing Lumbar Microdiscectomy: A Prospective Cross-Sectional Study

Autoři

CAPOOR, Manu N. (840 Spojené státy), Filip RŮŽIČKA (203 Česká republika, domácí), Táňa MACHÁČKOVÁ (203 Česká republika, domácí), Radim JANČÁLEK (203 Česká republika, domácí), Martin SMRČKA (203 Česká republika, domácí), Jonathan E. SCHMITZ (840 Spojené státy), Markéta HERMANOVÁ (203 Česká republika, domácí), Jiří ŠÁNA (203 Česká republika, domácí), Elleni PONECHAL MICHU (203 Česká republika, domácí), John C. BAIRD (203 Česká republika), Fahad S. AHMED (203 Česká republika), Karel MÁCA (203 Česká republika, domácí), Radim LIPINA (203 Česká republika), Todd F. ALAMIN (840 Spojené státy), Michael F. COSCIA (840 Spojené státy), Jerry L. STONEMETZ (840 Spojené státy), Timothy WITHAM (840 Spojené státy), Garth D. EHRLICH (840 Spojené státy), Ziya L. GOKASLAN (840 Spojené státy), Konstantinos MAVROMMATIS (840 Spojené státy), Christof BIRKENMAIER (276 Německo), Vincent A. FISCHETTI (840 Spojené státy) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

Plos one, San Francisco, Public Library of Science, 2016, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.806

Kód RIV

RIV/00216224:14110/16:00091845

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0161676

UT WoS

000381577000130

Klíčová slova anglicky

BACTERIAL-INFECTION; BIOFILM FORMATION; SKIN; ASSOCIATION; CONTAMINATION; DEGENERATION; SARCOIDOSIS; SCIATICA; SURGERY; SAMPLES

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 12. 2016 12:28, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Background The relationship between intervertebral disc degeneration and chronic infection by Propionibacterium acnes is controversial with contradictory evidence available in the literature. Previous studies investigating these relationships were under-powered and fraught with methodical differences; moreover, they have not taken into consideration P. acnes' ability to form biofilms or attempted to quantitate the bioburden with regard to determining bacterial counts/genome equivalents as criteria to differentiate true infection from contamination. The aim of this prospective cross-sectional study was to determine the prevalence of P. acnes in patients undergoing lumbar disc microdiscectomy. Methods and Findings The sample consisted of 290 adult patients undergoing lumbar microdiscectomy for symptomatic lumbar disc herniation. An intraoperative biopsy and pre-operative clinical data were taken in all cases. One biopsy fragment was homogenized and used for quantitative anaerobic culture and a second was frozen and used for real-time PCR-based quantification of P. acnes genomes. P. acnes was identified in 115 cases (40%), coagulase-negative staphylococci in 31 cases (11%) and alpha-hemolytic streptococci in 8 cases (3%). P. acnes counts ranged from 100 to 9000 CFU/ml with a median of 400 CFU/ml. The prevalence of intervertebral discs with abundant P. acnes (>= 1x10(3) CFU/ml) was 11% (39 cases). There was significant correlation between the bacterial counts obtained by culture and the number of P. acnes genomes detected by real-time PCR (r = 0.4363, p<0.0001). Conclusions In a large series of patients, the prevalence of discs with abundant P. acnes was 11%. We believe, disc tissue homogenization releases P. acnes from the biofilm so that they can then potentially be cultured, reducing the rate of false-negative cultures. Further, quantification study revealing significant bioburden based on both culture and real-time PCR minimize the likelihood that observed findings are due to contamination and supports the hypothesis P. acnes acts as a pathogen in these cases of degenerative disc disease.

Přiložené soubory

EL_1361474.pdf

Požádat o autorskou verzi souboru

Citovat

CAPOOR, Manu N., Filip RŮŽIČKA, Táňa MACHÁČKOVÁ, Radim JANČÁLEK, Martin SMRČKA, Jonathan E. SCHMITZ, Markéta HERMANOVÁ, Jiří ŠÁNA, Elleni PONECHAL MICHU, John C. BAIRD, Fahad S. AHMED, Karel MÁCA, Radim LIPINA, Todd F. ALAMIN, Michael F. COSCIA, Jerry L. STONEMETZ, Timothy WITHAM, Garth D. EHRLICH, Ziya L. GOKASLAN, Konstantinos MAVROMMATIS, Christof BIRKENMAIER, Vincent A. FISCHETTI a Ondřej SLABÝ. Prevalence of Propionibacterium acnes in Intervertebral Discs of Patients Undergoing Lumbar Microdiscectomy: A Prospective Cross-Sectional Study. Plos one. San Francisco: Public Library of Science, 2016, roč. 11, č. 8, s. 1-12. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0161676.

@article{1361474,
   author = {Capoor, Manu N. and Růžička, Filip and Macháčková, Táňa and Jančálek, Radim and Smrčka, Martin and Schmitz, Jonathan E. and Hermanová, Markéta and Šána, Jiří and Ponechal Michu, Elleni and Baird, John C. and Ahmed, Fahad S. and Máca, Karel and Lipina, Radim and Alamin, Todd F. and Coscia, Michael F. and Stonemetz, Jerry L. and Witham, Timothy and Ehrlich, Garth D. and Gokaslan, Ziya L. and Mavrommatis, Konstantinos and Birkenmaier, Christof and Fischetti, Vincent A. and Slabý, Ondřej},
   article_location = {San Francisco},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0161676},
   keywords = {BACTERIAL-INFECTION; BIOFILM FORMATION; SKIN; ASSOCIATION; CONTAMINATION; DEGENERATION; SARCOIDOSIS; SCIATICA; SURGERY; SAMPLES},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Prevalence of Propionibacterium acnes in Intervertebral Discs of Patients Undergoing Lumbar Microdiscectomy: A Prospective Cross-Sectional Study},
   volume = {11},
   year = {2016}
}

TY  - JOUR
ID  - 1361474
AU  - Capoor, Manu N. - Růžička, Filip - Macháčková, Táňa - Jančálek, Radim - Smrčka, Martin - Schmitz, Jonathan E. - Hermanová, Markéta - Šána, Jiří - Ponechal Michu, Elleni - Baird, John C. - Ahmed, Fahad S. - Máca, Karel - Lipina, Radim - Alamin, Todd F. - Coscia, Michael F. - Stonemetz, Jerry L. - Witham, Timothy - Ehrlich, Garth D. - Gokaslan, Ziya L. - Mavrommatis, Konstantinos - Birkenmaier, Christof - Fischetti, Vincent A. - Slabý, Ondřej
PY  - 2016
TI  - Prevalence of Propionibacterium acnes in Intervertebral Discs of Patients Undergoing Lumbar Microdiscectomy: A Prospective Cross-Sectional Study
JF  - Plos one
VL  - 11
IS  - 8
SP  - 1-12
EP  - 1-12
PB  - Public Library of Science
SN  - 19326203
KW  - BACTERIAL-INFECTION
KW  - BIOFILM FORMATION
KW  - SKIN
KW  - ASSOCIATION
KW  - CONTAMINATION
KW  - DEGENERATION
KW  - SARCOIDOSIS
KW  - SCIATICA
KW  - SURGERY
KW  - SAMPLES
N2  - Background The relationship between intervertebral disc degeneration and chronic infection by Propionibacterium acnes is controversial with contradictory evidence available in the literature. Previous studies investigating these relationships were under-powered and fraught with methodical differences; moreover, they have not taken into consideration P. acnes' ability to form biofilms or attempted to quantitate the bioburden with regard to determining bacterial counts/genome equivalents as criteria to differentiate true infection from contamination. The aim of this prospective cross-sectional study was to determine the prevalence of P. acnes in patients undergoing lumbar disc microdiscectomy. Methods and Findings The sample consisted of 290 adult patients undergoing lumbar microdiscectomy for symptomatic lumbar disc herniation. An intraoperative biopsy and pre-operative clinical data were taken in all cases. One biopsy fragment was homogenized and used for quantitative anaerobic culture and a second was frozen and used for real-time PCR-based quantification of P. acnes genomes. P. acnes was identified in 115 cases (40%), coagulase-negative staphylococci in 31 cases (11%) and alpha-hemolytic streptococci in 8 cases (3%). P. acnes counts ranged from 100 to 9000 CFU/ml with a median of 400 CFU/ml. The prevalence of intervertebral discs with abundant P. acnes (>= 1x10(3) CFU/ml) was 11% (39 cases). There was significant correlation between the bacterial counts obtained by culture and the number of P. acnes genomes detected by real-time PCR (r = 0.4363, p<0.0001). Conclusions In a large series of patients, the prevalence of discs with abundant P. acnes was 11%. We believe, disc tissue homogenization releases P. acnes from the biofilm so that they can then potentially be cultured, reducing the rate of false-negative cultures. Further, quantification study revealing significant bioburden based on both culture and real-time PCR minimize the likelihood that observed findings are due to contamination and supports the hypothesis P. acnes acts as a pathogen in these cases of degenerative disc disease.
ER  -

CAPOOR, Manu N., Filip RŮŽIČKA, Táňa MACHÁČKOVÁ, Radim JANČÁLEK, Martin SMRČKA, Jonathan E. SCHMITZ, Markéta HERMANOVÁ, Jiří ŠÁNA, Elleni PONECHAL MICHU, John C. BAIRD, Fahad S. AHMED, Karel MÁCA, Radim LIPINA, Todd F. ALAMIN, Michael F. COSCIA, Jerry L. STONEMETZ, Timothy WITHAM, Garth D. EHRLICH, Ziya L. GOKASLAN, Konstantinos MAVROMMATIS, Christof BIRKENMAIER, Vincent A. FISCHETTI a Ondřej SLABÝ. Prevalence of Propionibacterium acnes in Intervertebral Discs of Patients Undergoing Lumbar Microdiscectomy: A Prospective Cross-Sectional Study. \textit{Plos one}. San Francisco: Public Library of Science, 2016, roč.~11, č.~8, s.~1-12. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0161676.

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