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@article{1361878,
author = {Urík, Milan and Hurník, Pavel and Žiak, D. and Machač, Josef and Šlapák, Ivo and Motyka, O. and Vaculová, J. and Dvořáčková, J.},
article_location = {Amsterdam},
article_number = {JUL 2016},
keywords = {Retraction pockets; Histological analysis; Cholesteatoma; Children},
language = {eng},
issn = {0165-5876},
journal = {International journal of pediatric otorhinolaryngology},
title = {Histological analysis of retraction pocket pars tensa of tympanic membrane in children},
volume = {86},
year = {2016}
}
TY - JOUR
ID - 1361878
AU - Urík, Milan - Hurník, Pavel - Žiak, D. - Machač, Josef - Šlapák, Ivo - Motyka, O. - Vaculová, J. - Dvořáčková, J.
PY - 2016
TI - Histological analysis of retraction pocket pars tensa of tympanic membrane in children
JF - International journal of pediatric otorhinolaryngology
VL - 86
IS - JUL 2016
SP - 213-217
EP - 213-217
PB - Elsevier
SN - 01655876
KW - Retraction pockets
KW - Histological analysis
KW - Cholesteatoma
KW - Children
N2 - Aims: Histological and histochemical analysis of retraction pocket of pars tensa of tympanic membrane in children. Identification of morphological abnormalities in comparison with a healthy tympanic membrane as it is described in standard textbook. Identification of signs typical for cholesteatoma and support for a retraction theory of cholesteatoma formation. Study design: A prospective study analysing 31 samples of retraction pockets taken during surgery. Departments: University Hospital, Children's Medical Centre Methods: Samples of retraction pockets were processed by a standard process for light microscopy, stained by haematoxylin-eosin. Van Gieson's stain was used for differential staining of collagen, Verhoeff's stain for elastic fibre tissues, Alcian blue for acidic polysaccharides and PAS (Periodic Acid Schiff) method for basement membrane polysaccharides. Results: The following findings were observed in the samples of retraction pockets: hyperkeratosis (100%), hypervascularisations (100%), subepithelial fragmented elastic fibres (96%), myxoid changes (87%), sub epithelial inflammatory infiltration (84%), rete pegs (71%), papilomatosis (71%), intraepithelial inflammatory cellularizations, (48%), intraepithelial spongiosis (16%) and parakeratosis (3%). No basement membrane continuity interruptions were observed. Thickness of retraction pocket, thickness of epidermis, occurrence of rete pegs and frequency of fragmented elastic fibres was higher in a Grade III stage RP than Grade II stage RP (according to Charachon). Conclusion: Morphological abnormalities in the structure of retraction pockets in comparison with a healthy tympanic membrane were described. The changes are typical for a structure of cholesteatoma (these changes are common in matrix and perimatrix), supporting retraction theory of its origin. Our observations show that it is inflammation that probably plays a key role in the pathogenesis of retraction pocket. The frequency of some of the changes increases with the stage of retraction pocket according to Charachon). Basement membrane continuity interruptions are not typical for retraction pockets.
ER -
URÍK, Milan, Pavel HURNÍK, D. ŽIAK, Josef MACHAČ, Ivo ŠLAPÁK, O. MOTYKA, J. VACULOVÁ a J. DVOŘÁČKOVÁ. Histological analysis of retraction pocket pars tensa of tympanic membrane in children. \textit{International journal of pediatric otorhinolaryngology}. Amsterdam: Elsevier, 2016, roč.~86, JUL 2016, s.~213-217. ISSN~0165-5876.
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