Bacterial community dynamics in a rumen fluid bioreactor during
in-vitro cultivation

J 2016

Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation

ZAPLETALOVÁ, Martina, Jitka KAŠPAROVSKÁ, Ludmila KŘÍŽOVÁ, Tomáš KAŠPAROVSKÝ, Omar ŠERÝ et. al.

Basic information

Original name

Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation

Authors

ZAPLETALOVÁ, Martina (203 Czech Republic, belonging to the institution), Jitka KAŠPAROVSKÁ (203 Czech Republic, belonging to the institution), Ludmila KŘÍŽOVÁ (203 Czech Republic, belonging to the institution), Tomáš KAŠPAROVSKÝ (203 Czech Republic, belonging to the institution), Omar ŠERÝ (203 Czech Republic, belonging to the institution) and Jan LOCHMAN (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Biotechnology, Amsterdam, Elsevier, 2016, 0168-1656

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.599

RIV identification code

RIV/00216224:14310/16:00088379

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.jbiotec.2016.07.013

UT WoS

000382193600005

Keywords in English

Rumen fluid; Bioreactor; Redox potential; Microbiome analysis; 16S rDNA

Abstract

V originále

To study the various processes in the rumen the in vitro techniques are widely used to realize more controlled and reproducible conditions compared to in vivo experiments. Mostly, only the parameters like pH changes, volatile fatty acids content or metabolite production are monitored. In this study we examine the bacterial community dynamics of rumen fluid in course of ten day cultivation realize under standard conditions described in the literature. Whereas the pH values, total VFA content and A/P ratio in bioreactor were consistent with natural conditions in the rumen, the mean redox-potential values of 251 and 243 mV were much more negative. For culture-independent assessment of bacterial community composition, the Illumina MiSeq results indicated that the community contained 292 bacterial genera. In course of ten days cultivation a significant changes in the microbial community were measured when Bacteroidetes to Firmicutes ratio changed from 3.2 to 1.2 and phyla Proteo bacteria and Actino bacteria represented by genus Bifido bacterium and Olsenella significantly increased. The main responsible factor of these changes seems to be very low redox potential in bioreactor together with accumulation of simple carbohydrates in milieu as a result of limited excretion of fermented feed and absence of nutrient absorbing mechanisms. (C) 2016 Elsevier B.V. All rights reserved.

Links

GA13-34134S, research and development project

Name: Metabolizmus izoflavonů v bachoru se zaměřením na obsah equolu v kravském mléce

Investor: Czech Science Foundation

MUNI/A/1265/2015, interní kód MU

Name: Podpora biochemického výzkumu v roce 2016

Investor: Masaryk University, Category A

Citovat

ZAPLETALOVÁ, Martina, Jitka KAŠPAROVSKÁ, Ludmila KŘÍŽOVÁ, Tomáš KAŠPAROVSKÝ, Omar ŠERÝ and Jan LOCHMAN. Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation. Journal of Biotechnology. Amsterdam: Elsevier, 2016, vol. 234, september, p. 43-49. ISSN 0168-1656. Available from: https://dx.doi.org/10.1016/j.jbiotec.2016.07.013.

@article{1362002,
   author = {Zapletalová, Martina and Kašparovská, Jitka and Křížová, Ludmila and Kašparovský, Tomáš and Šerý, Omar and Lochman, Jan},
   article_location = {Amsterdam},
   article_number = {september},
   doi = {http://dx.doi.org/10.1016/j.jbiotec.2016.07.013},
   keywords = {Rumen fluid; Bioreactor; Redox potential; Microbiome analysis; 16S rDNA},
   language = {eng},
   issn = {0168-1656},
   journal = {Journal of Biotechnology},
   title = {Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation},
   volume = {234},
   year = {2016}
}

TY  - JOUR
ID  - 1362002
AU  - Zapletalová, Martina - Kašparovská, Jitka - Křížová, Ludmila - Kašparovský, Tomáš - Šerý, Omar - Lochman, Jan
PY  - 2016
TI  - Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation
JF  - Journal of Biotechnology
VL  - 234
IS  - september
SP  - 43-49
EP  - 43-49
PB  - Elsevier
SN  - 01681656
KW  - Rumen fluid
KW  - Bioreactor
KW  - Redox potential
KW  - Microbiome analysis
KW  - 16S rDNA
N2  - To study the various processes in the rumen the in vitro techniques are widely used to realize more controlled and reproducible conditions compared to in vivo experiments. Mostly, only the parameters like pH changes, volatile fatty acids content or metabolite production are monitored. In this study we examine the bacterial community dynamics of rumen fluid in course of ten day cultivation realize under standard conditions described in the literature. Whereas the pH values, total VFA content and A/P ratio in bioreactor were consistent with natural conditions in the rumen, the mean redox-potential values of 251 and 243 mV were much more negative. For culture-independent assessment of bacterial community composition, the Illumina MiSeq results indicated that the community contained 292 bacterial genera. In course of ten days cultivation a significant changes in the microbial community were measured when Bacteroidetes to Firmicutes ratio changed from 3.2 to 1.2 and phyla Proteo bacteria and Actino bacteria represented by genus Bifido bacterium and Olsenella significantly increased. The main responsible factor of these changes seems to be very low redox potential in bioreactor together with accumulation of simple carbohydrates in milieu as a result of limited excretion of fermented feed and absence of nutrient absorbing mechanisms. (C) 2016 Elsevier B.V. All rights reserved.
ER  -

ZAPLETALOVÁ, Martina, Jitka KAŠPAROVSKÁ, Ludmila KŘÍŽOVÁ, Tomáš KAŠPAROVSKÝ, Omar ŠERÝ and Jan LOCHMAN. Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation. \textit{Journal of Biotechnology}. Amsterdam: Elsevier, 2016, vol.~234, september, p.~43-49. ISSN~0168-1656. Available from: https://dx.doi.org/10.1016/j.jbiotec.2016.07.013.

Detailed Information on Publication Record