Glucose determination in fish plasma by two different moderate
methods

J 2016

Glucose determination in fish plasma by two different moderate methods

BARTOŇKOVÁ, Jana, Pavel HYRŠL a Libor VOJTEK

Základní údaje

Originální název

Glucose determination in fish plasma by two different moderate methods

Autoři

BARTOŇKOVÁ, Jana (203 Česká republika, domácí), Pavel HYRŠL (203 Česká republika, domácí) a Libor VOJTEK (203 Česká republika, garant, domácí)

Vydání

Acta Veterinaria, Brno, VETERINARNI A FARMACEUTICKA UNIVERZITA B, 2016, 0001-7213

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.415

Kód RIV

RIV/00216224:14310/16:00092118

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.2754/avb201685040349

UT WoS

000398194600005

Klíčová slova česky

glukometr; spectrofotometrie; lín; Tinca tinca

Klíčová slova anglicky

glucometer; spectrophotometry; tench; Tinca tinca

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2020 16:36, Mgr. Marie Šípková, DiS.

Anotace

V originále

Group of teleost fish consist from many different species and has large economic, societal and environmental impact. Glucose is the main source of energy for most vertebrate organisms. Fish as well as for example mammals maintain specific level of blood sugar – glycaemia, which is influenced by multiple factors such as hormone production, diet and temperature. Therefore, it is favourable parameter of scientists to check the immediate state of an organism. Most utilized method to measure glycaemia in farmed fish is spectrophotometry, however for field experiments is this method often inconvenient. Here we report that basic spectrophotometry using oxidation of blood beta D-glucose by glucose oxidase (used in laboratory conditions) can be easily substituted with portable glucometer measurements, primarily designed for human samples. This is very useful thanks to the possibility of use outside the laboratory and its small sample volume demands. Terrain studies often require fast sample processing and conditions of measurement are limited. Using glucometer is very simple and elegant option. We have tested 74 blood plasma samples (29 males, 45 females) of tench (Tinca tinca) both spectrophotometrically and using human glucometer. Our results show significant linear correlation (p < 0.001) between both methods. The main contribution of this study consists in comparability and interchangeability of results obtained from both presented methods.

Citovat

BARTOŇKOVÁ, Jana, Pavel HYRŠL a Libor VOJTEK. Glucose determination in fish plasma by two different moderate methods. Acta Veterinaria. Brno: VETERINARNI A FARMACEUTICKA UNIVERZITA B, 2016, roč. 85, č. 4, s. 349-353. ISSN 0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb201685040349.

@article{1363171,
   author = {Bartoňková, Jana and Hyršl, Pavel and Vojtek, Libor},
   article_location = {Brno},
   article_number = {4},
   doi = {http://dx.doi.org/10.2754/avb201685040349},
   keywords = {glucometer; spectrophotometry; tench; Tinca tinca},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta Veterinaria},
   title = {Glucose determination in fish plasma by two different moderate methods},
   url = {http://dx.doi.org/10.2754/avb201685040349},
   volume = {85},
   year = {2016}
}

TY  - JOUR
ID  - 1363171
AU  - Bartoňková, Jana - Hyršl, Pavel - Vojtek, Libor
PY  - 2016
TI  - Glucose determination in fish plasma by two different moderate methods
JF  - Acta Veterinaria
VL  - 85
IS  - 4
SP  - 349-353
EP  - 349-353
PB  - VETERINARNI A FARMACEUTICKA UNIVERZITA B
SN  - 00017213
KW  - glucometer
KW  - spectrophotometry
KW  - tench
KW  - Tinca tinca
UR  - http://dx.doi.org/10.2754/avb201685040349
L2  - http://dx.doi.org/10.2754/avb201685040349
N2  - Group of teleost fish consist from many different species and has large economic, societal and environmental impact. Glucose is the main source of energy for most vertebrate organisms. Fish as well as for example mammals maintain specific level of blood sugar – glycaemia, which is influenced by multiple factors such as hormone production, diet and temperature. Therefore, it is favourable parameter of scientists to check the immediate state of an organism. Most utilized method to measure glycaemia in farmed fish is spectrophotometry, however for field experiments is this method often inconvenient. Here we report that basic spectrophotometry using oxidation of blood beta D-glucose by glucose oxidase (used in laboratory conditions) can be easily substituted with portable glucometer measurements, primarily designed for human samples. This is very useful thanks to the possibility of use outside the laboratory and its small sample volume demands. Terrain studies often require fast sample processing and conditions of measurement are limited. Using glucometer is very simple and elegant option. We have tested 74 blood plasma samples (29 males, 45 females) of tench (Tinca tinca) both spectrophotometrically and using human glucometer. Our results show significant linear correlation (p < 0.001) between both methods. The main contribution of this study consists in comparability and interchangeability of results obtained from both presented methods.
ER  -

BARTOŇKOVÁ, Jana, Pavel HYRŠL a Libor VOJTEK. Glucose determination in fish plasma by two different moderate methods. \textit{Acta Veterinaria}. Brno: VETERINARNI A FARMACEUTICKA UNIVERZITA B, 2016, roč.~85, č.~4, s.~349-353. ISSN~0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb201685040349.

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