Simultaneous Determination of Uric Acid, Xanthine and
Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid
Metabolism Tracking

J 2017

Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking

PLESKAČOVÁ, Anna, Stanislav BREJCHA, Lukáš PÁCAL, Kateřina KAŇKOVÁ, Josef TOMANDL et. al.

Základní údaje

Originální název

Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking

Autoři

PLESKAČOVÁ, Anna (203 Česká republika, domácí), Stanislav BREJCHA (203 Česká republika, domácí), Lukáš PÁCAL (203 Česká republika, domácí), Kateřina KAŇKOVÁ (203 Česká republika, domácí) a Josef TOMANDL (203 Česká republika, garant, domácí)

Vydání

Chromatographia, Heidelberg, Springer, 2017, 0009-5893

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.401

Kód RIV

RIV/00216224:14110/17:00095624

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s10337-016-3208-8

UT WoS

000399024500003

Klíčová slova anglicky

HPLC; Uric; acid; Xanthine; Hypoxanthine; Plasma; Serum

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2018 16:34, Soňa Böhmová

Anotace

V originále

A simple reversed phase HPLC method with UV detection in isocratic conditions was developed and validated for the simultaneous determination of hypoxanthine, xanthine and uric acid levels in human plasma and serum. One analysis run takes 6.5 min including a short organic mobile phase gradient for column regeneration. Concentrations of uric acid, xanthine and hypoxanthine in plasma and serum samples were highly comparable. However, hypoxanthine levels were increased in serum compared to plasma samples due to a prolonged time between serum and blood elements separation. The method was validated for linearity, precision, accuracy, sensitivity and robustness in a similar manner to that for pharmacokinetic data and it is appropriate for physiological and pathophysiological levels of all analytes. The stability of stock standard solutions was verified using spectrophotometric analysis in different conditions. The method is simple and robust with a good precision for the measurement of hypoxanthine, xanthine and uric acid in human plasma and serum.

Návaznosti

MUNI/A/1056/2015, interní kód MU

Název: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Akronym: bichepat)

Investor: Masarykova univerzita, Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

NV16-28040A, projekt VaV

Název: Dlouhodobé dopady gestačního diabetes mellitus pro metabolické zdraví žen časně postpartum: význam nových diagnostických kritérií

Citovat

PLESKAČOVÁ, Anna, Stanislav BREJCHA, Lukáš PÁCAL, Kateřina KAŇKOVÁ a Josef TOMANDL. Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking. Chromatographia. Heidelberg: Springer, 2017, roč. 80, č. 4, s. 529-536. ISSN 0009-5893. Dostupné z: https://dx.doi.org/10.1007/s10337-016-3208-8.

@article{1363208,
   author = {Pleskačová, Anna and Brejcha, Stanislav and Pácal, Lukáš and Kaňková, Kateřina and Tomandl, Josef},
   article_location = {Heidelberg},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s10337-016-3208-8},
   keywords = {HPLC; Uric; acid; Xanthine; Hypoxanthine; Plasma; Serum},
   language = {eng},
   issn = {0009-5893},
   journal = {Chromatographia},
   title = {Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking},
   volume = {80},
   year = {2017}
}

TY  - JOUR
ID  - 1363208
AU  - Pleskačová, Anna - Brejcha, Stanislav - Pácal, Lukáš - Kaňková, Kateřina - Tomandl, Josef
PY  - 2017
TI  - Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking
JF  - Chromatographia
VL  - 80
IS  - 4
SP  - 529-536
EP  - 529-536
PB  - Springer
SN  - 00095893
KW  - HPLC
KW  - Uric
KW  - acid
KW  - Xanthine
KW  - Hypoxanthine
KW  - Plasma
KW  - Serum
N2  - A simple reversed phase HPLC method with UV detection in isocratic conditions was developed and validated for the simultaneous determination of hypoxanthine, xanthine and uric acid levels in human plasma and serum. One analysis run takes 6.5 min including a short organic mobile phase gradient for column regeneration. Concentrations of uric acid, xanthine and hypoxanthine in plasma and serum samples were highly comparable. However, hypoxanthine levels were increased in serum compared to plasma samples due to a prolonged time between serum and blood elements separation. The method was validated for linearity, precision, accuracy, sensitivity and robustness in a similar manner to that for pharmacokinetic data and it is appropriate for physiological and pathophysiological levels of all analytes. The stability of stock standard solutions was verified using spectrophotometric analysis in different conditions. The method is simple and robust with a good precision for the measurement of hypoxanthine, xanthine and uric acid in human plasma and serum.
ER  -

PLESKAČOVÁ, Anna, Stanislav BREJCHA, Lukáš PÁCAL, Kateřina KAŇKOVÁ a Josef TOMANDL. Simultaneous Determination of Uric Acid, Xanthine and Hypoxanthine in Human Plasma and Serum by HPLC–UV: Uric Acid Metabolism Tracking. \textit{Chromatographia}. Heidelberg: Springer, 2017, roč.~80, č.~4, s.~529-536. ISSN~0009-5893. Dostupné z: https://dx.doi.org/10.1007/s10337-016-3208-8.

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