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@inbook{1363692,
author = {Koledová, Zuzana and Lu, Pengfei},
address = {New York},
booktitle = {Mammary Gland Development: Methods and Protocols},
doi = {http://dx.doi.org/10.1007/978-1-4939-6475-8_10},
edition = {1st ed},
editor = {Finian Martin, Torsten Stein, Jillian Howlin},
keywords = {3D culture; Branching morphogenesis; Extracellular matrix; Fibroblasts; Mammary epithelium; Matrigel; Organoids; Organotypic assay; Paracrine signaling},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York},
isbn = {978-1-4939-6473-4},
pages = {217-231},
publisher = {Springer New York - Humana Press},
title = {A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland},
year = {2017}
}
TY - CHAP
ID - 1363692
AU - Koledová, Zuzana - Lu, Pengfei
PY - 2017
TI - A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland
VL - Methods in Molecular Biology, vol. 1501
PB - Springer New York - Humana Press
CY - New York
SN - 9781493964734
KW - 3D culture
KW - Branching morphogenesis
KW - Extracellular matrix
KW - Fibroblasts
KW - Mammary epithelium
KW - Matrigel
KW - Organoids
KW - Organotypic assay
KW - Paracrine signaling
N2 - The mammary gland consists of numerous tissue compartments, including mammary epithelium, an array of stromal cells, and the extracellular matrix (ECM). Bidirectional interactions between the epithelium and its surrounding stroma are essential for proper mammary gland development and homeostasis, whereas their deregulation leads to developmental abnormalities and cancer. To study the relationships between the epithelium and the stroma, development of models that could recapitulate essential aspects of these interacting systems in vitro has become necessary. Here we describe a three-dimensional (3D) co-culture assay and show that the addition of fibroblasts to mammary organoid cultures promotes the epithelium to undergo branching morphogenesis, thus allowing the role of the stromal microenvironment to be examined in this essential developmental process.
ER -
KOLEDOVÁ, Zuzana a Pengfei LU. A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland. In Finian Martin, Torsten Stein, Jillian Howlin. \textit{Mammary Gland Development: Methods and Protocols}. 1st ed. New York: Springer New York - Humana Press, 2017, s.~217-231. Methods in Molecular Biology, vol. 1501. ISBN~978-1-4939-6473-4. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-6475-8\_{}10.
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