A 3D Fibroblast-Epithelium Co-culture Model for Understanding
Microenvironmental Role in Branching Morphogenesis of the
Mammary Gland

C 2017

A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland

KOLEDOVÁ, Zuzana a Pengfei LU

Základní údaje

Originální název

A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland

Autoři

KOLEDOVÁ, Zuzana (703 Slovensko, garant, domácí) a Pengfei LU (156 Čína)

Vydání

1st ed. New York, Mammary Gland Development: Methods and Protocols, od s. 217-231, 15 s. Methods in Molecular Biology, vol. 1501, 2017

Nakladatel

Springer New York - Humana Press

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10603 Genetics and heredity

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Kód RIV

RIV/00216224:14110/17:00095957

Organizační jednotka

Lékařská fakulta

ISBN

978-1-4939-6473-4

DOI

http://dx.doi.org/10.1007/978-1-4939-6475-8_10

UT WoS

000400932000011

Klíčová slova anglicky

3D culture; Branching morphogenesis; Extracellular matrix; Fibroblasts; Mammary epithelium; Matrigel; Organoids; Organotypic assay; Paracrine signaling

Štítky

EL OK, topvydavatel

Změněno: 27. 4. 2020 10:09, Mgr. Tereza Miškechová

Anotace

V originále

The mammary gland consists of numerous tissue compartments, including mammary epithelium, an array of stromal cells, and the extracellular matrix (ECM). Bidirectional interactions between the epithelium and its surrounding stroma are essential for proper mammary gland development and homeostasis, whereas their deregulation leads to developmental abnormalities and cancer. To study the relationships between the epithelium and the stroma, development of models that could recapitulate essential aspects of these interacting systems in vitro has become necessary. Here we describe a three-dimensional (3D) co-culture assay and show that the addition of fibroblasts to mammary organoid cultures promotes the epithelium to undergo branching morphogenesis, thus allowing the role of the stromal microenvironment to be examined in this essential developmental process.

Návaznosti

EE2.3.30.0037, projekt VaV

Název: Zaměstnáním nejlepších mladých vědců k rozvoji mezinárodní spolupráce

ROZV/20/LF/2015, interní kód MU

Název: LF - Příspěvek IP 2015

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek IP 2015

Citovat

KOLEDOVÁ, Zuzana a Pengfei LU. A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland. In Finian Martin, Torsten Stein, Jillian Howlin. Mammary Gland Development: Methods and Protocols. 1st ed. New York: Springer New York - Humana Press, 2017, s. 217-231. Methods in Molecular Biology, vol. 1501. ISBN 978-1-4939-6473-4. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-6475-8_10.

@inbook{1363692,
   author = {Koledová, Zuzana and Lu, Pengfei},
   address = {New York},
   booktitle = {Mammary Gland Development: Methods and Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-4939-6475-8_10},
   edition = {1st ed},
   editor = {Finian Martin, Torsten Stein, Jillian Howlin},
   keywords = {3D culture; Branching morphogenesis; Extracellular matrix; Fibroblasts; Mammary epithelium; Matrigel; Organoids; Organotypic assay; Paracrine signaling},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-4939-6473-4},
   pages = {217-231},
   publisher = {Springer New York - Humana Press},
   title = {A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland},
   year = {2017}
}

TY  - CHAP
ID  - 1363692
AU  - Koledová, Zuzana - Lu, Pengfei
PY  - 2017
TI  - A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland
VL  - Methods in Molecular Biology, vol. 1501
PB  - Springer New York - Humana Press
CY  - New York
SN  - 9781493964734
KW  - 3D culture
KW  - Branching morphogenesis
KW  - Extracellular matrix
KW  - Fibroblasts
KW  - Mammary epithelium
KW  - Matrigel
KW  - Organoids
KW  - Organotypic assay
KW  - Paracrine signaling
N2  - The mammary gland consists of numerous tissue compartments, including mammary epithelium, an array of stromal cells, and the extracellular matrix (ECM). Bidirectional interactions between the epithelium and its surrounding stroma are essential for proper mammary gland development and homeostasis, whereas their deregulation leads to developmental abnormalities and cancer. To study the relationships between the epithelium and the stroma, development of models that could recapitulate essential aspects of these interacting systems in vitro has become necessary. Here we describe a three-dimensional (3D) co-culture assay and show that the addition of fibroblasts to mammary organoid cultures promotes the epithelium to undergo branching morphogenesis, thus allowing the role of the stromal microenvironment to be examined in this essential developmental process.
ER  -

KOLEDOVÁ, Zuzana a Pengfei LU. A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland. In Finian Martin, Torsten Stein, Jillian Howlin. \textit{Mammary Gland Development: Methods and Protocols}. 1st ed. New York: Springer New York - Humana Press, 2017, s.~217-231. Methods in Molecular Biology, vol. 1501. ISBN~978-1-4939-6473-4. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-6475-8\_{}10.

Podrobný výpis o publikaci