Epithelial-mesenchymal transition-associated microRNA/mRNA
signature is linked to metastasis and prognosis in clear-cell
renal cell carcinoma

J 2016

Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma

MLČOCHOVÁ, Hana, Táňa MACHÁČKOVÁ, Anja RABIEN, Lenka RADOVÁ, Pavel FABIAN et. al.

Základní údaje

Originální název

Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma

Autoři

MLČOCHOVÁ, Hana (203 Česká republika, domácí), Táňa MACHÁČKOVÁ (203 Česká republika, domácí), Anja RABIEN (276 Německo), Lenka RADOVÁ (203 Česká republika, domácí), Pavel FABIAN (203 Česká republika), Robert ILIEV (203 Česká republika, domácí), Kateřina SLABÁ (203 Česká republika, domácí), Alexandr POPRACH (203 Česká republika, domácí), Ergin KILIC (276 Německo), Michal STANIK (203 Česká republika), Martina LOJOVÁ (203 Česká republika, domácí), Marek SVOBODA (203 Česká republika, domácí), Jan DOLEZEL (203 Česká republika), Rostislav VYZULA (203 Česká republika, domácí), Klaus JUNG (276 Německo) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

Scientific Reports, LONDON, NATURE PUBLISHING GROUP, 2016, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.259

Kód RIV

RIV/00216224:14740/16:00088919

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/srep31852

UT WoS

000381767500001

Klíčová slova anglicky

CANCER; GENE; EXPRESSION; PATHWAYS; TARGETS; PROTEIN; FREM1

Štítky

rivok

Příznaky

Recenzováno

Změněno: 26. 4. 2021 13:30, Mgr. Tereza Miškechová

Anotace

V originále

Clear-cell renal cell carcinomas (ccRCCs) are genetically heterogeneous tumors presenting diverse clinical courses. Epithelial-mesenchymal transition (EMT) is a crucial process involved in initiation of metastatic cascade. The aim of our study was to identify an integrated miRNA/mRNA signature associated with metastasis and prognosis in ccRCC through targeted approach based on analysis of miRNAs/mRNAs associated with EMT. A cohort of 230 ccRCC was included in our study and further divided into discovery, training and validation cohorts. EMT markers were evaluated in ccRCC tumor samples, which were grouped accordingly to EMT status. By use of large-scale miRNA/mRNA expression profiling, we identified miRNA/mRNA with significantly different expression in EMT-positive tumors and selected 41 miRNAs/mRNAs for training phase of the study to evaluate their diagnostic and prognostic potential. Fifteen miRNAs/mRNAs were analyzed in the validation phase, where all evaluated miRNA/mRNA candidates were confirmed to be significantly deregulated in tumor tissue. Some of them significantly differed in metastatic tumors, correlated with clinical stage, with Fuhrman grade and with overall survival. Further, we established an EMT-based stage-independent prognostic scoring system enabling identification of ccRCC patients at high-risk of cancer-related death. Finally, we confirmed involvement of miR-429 in EMT regulation in RCC cells in vitro.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NT13547, projekt VaV

Název: Studium mikroRNA a genů asociovaných s procesem epiteliálně-mezenchymální tranzice jako potenciálních markerů pro predikci rizika a časný záchyt metastazování u pacientů s renálním karcinomem

90089, velká výzkumná infrastruktura

Název: BBMRI-CZ II

Citovat

MLČOCHOVÁ, Hana, Táňa MACHÁČKOVÁ, Anja RABIEN, Lenka RADOVÁ, Pavel FABIAN, Robert ILIEV, Kateřina SLABÁ, Alexandr POPRACH, Ergin KILIC, Michal STANIK, Martina LOJOVÁ, Marek SVOBODA, Jan DOLEZEL, Rostislav VYZULA, Klaus JUNG a Ondřej SLABÝ. Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma. Scientific Reports. LONDON: NATURE PUBLISHING GROUP, 2016, roč. 6, August, s. nestránkováno, 12 s. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep31852.

@article{1363865,
   author = {Mlčochová, Hana and Macháčková, Táňa and Rabien, Anja and Radová, Lenka and Fabian, Pavel and Iliev, Robert and Slabá, Kateřina and Poprach, Alexandr and Kilic, Ergin and Stanik, Michal and Lojová, Martina and Svoboda, Marek and Dolezel, Jan and Vyzula, Rostislav and Jung, Klaus and Slabý, Ondřej},
   article_location = {LONDON},
   article_number = {August},
   doi = {http://dx.doi.org/10.1038/srep31852},
   keywords = {CANCER; GENE; EXPRESSION; PATHWAYS; TARGETS; PROTEIN; FREM1},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma},
   url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994011/pdf/srep31852.pdf},
   volume = {6},
   year = {2016}
}

TY  - JOUR
ID  - 1363865
AU  - Mlčochová, Hana - Macháčková, Táňa - Rabien, Anja - Radová, Lenka - Fabian, Pavel - Iliev, Robert - Slabá, Kateřina - Poprach, Alexandr - Kilic, Ergin - Stanik, Michal - Lojová, Martina - Svoboda, Marek - Dolezel, Jan - Vyzula, Rostislav - Jung, Klaus - Slabý, Ondřej
PY  - 2016
TI  - Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma
JF  - Scientific Reports
VL  - 6
IS  - August
SP  - nestránkováno
EP  - nestránkováno
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - CANCER
KW  - GENE
KW  - EXPRESSION
KW  - PATHWAYS
KW  - TARGETS
KW  - PROTEIN
KW  - FREM1
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994011/pdf/srep31852.pdf
L2  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4994011/pdf/srep31852.pdf
N2  - Clear-cell renal cell carcinomas (ccRCCs) are genetically heterogeneous tumors presenting diverse clinical courses. Epithelial-mesenchymal transition (EMT) is a crucial process involved in initiation of metastatic cascade. The aim of our study was to identify an integrated miRNA/mRNA signature associated with metastasis and prognosis in ccRCC through targeted approach based on analysis of miRNAs/mRNAs associated with EMT. A cohort of 230 ccRCC was included in our study and further divided into discovery, training and validation cohorts. EMT markers were evaluated in ccRCC tumor samples, which were grouped accordingly to EMT status. By use of large-scale miRNA/mRNA expression profiling, we identified miRNA/mRNA with significantly different expression in EMT-positive tumors and selected 41 miRNAs/mRNAs for training phase of the study to evaluate their diagnostic and prognostic potential. Fifteen miRNAs/mRNAs were analyzed in the validation phase, where all evaluated miRNA/mRNA candidates were confirmed to be significantly deregulated in tumor tissue. Some of them significantly differed in metastatic tumors, correlated with clinical stage, with Fuhrman grade and with overall survival. Further, we established an EMT-based stage-independent prognostic scoring system enabling identification of ccRCC patients at high-risk of cancer-related death. Finally, we confirmed involvement of miR-429 in EMT regulation in RCC cells in vitro.
ER  -

MLČOCHOVÁ, Hana, Táňa MACHÁČKOVÁ, Anja RABIEN, Lenka RADOVÁ, Pavel FABIAN, Robert ILIEV, Kateřina SLABÁ, Alexandr POPRACH, Ergin KILIC, Michal STANIK, Martina LOJOVÁ, Marek SVOBODA, Jan DOLEZEL, Rostislav VYZULA, Klaus JUNG a Ondřej SLABÝ. Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, 2016, roč.~6, August, s.~nestránkováno, 12 s. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep31852.

Podrobný výpis o publikaci