Enzymatic Precipitation Enhanced Surface Plasmon Resonance
Immunosensor for the Detection of Salmonella in Powdered Milk

J 2016

Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA a Petr SKLÁDAL

Základní údaje

Originální název

Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk

Autoři

FARKA, Zdeněk (203 Česká republika, domácí), Tomáš JUŘÍK (203 Česká republika, domácí), Matěj PASTUCHA (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

Analytical Chemistry, Washington, DC, American Chemical Society, 2016, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.320

Kód RIV

RIV/00216224:14740/16:00092189

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1021/acs.analchem.6b03511

UT WoS

000389556900080

Klíčová slova anglicky

Surface plasmon resonance biosensor; Biocatalyzed precipitation; Food safety; Bacterial pathogen; Salmonella Typhimurium; Atomic force microscopy

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 9. 2018 18:50, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Contamination of food by pathogenic bacteria has always been a serious threat for human health. The amount of food exports and imports has been increasing in recent years which requires precise food quality control with short analysis time and simplified sample treatment. Surface plasmon resonance (SPR) immunosensor enhanced by biocatalyzed precipitation was developed for the analysis of Salmonella in dairy products. The specific capture antibody was immobilized on the SPR chip which allowed a direct label-free detection of Salmonella Typhimurium with the limit of detection (LOD) of 104 CFU·mL–1 and the analysis time of 10 min. Alternatively, the secondary detection antibody was conjugated with horseradish peroxidase to provide a signal enhancement by the biocatalyzed conversion of 4-chloro-1-naphthol to insoluble benzo-4-chlorocyclohexadienone. The formation of precipitate was studied in detail by atomic force microscopy (AFM). The sensitivity was increased 40 times in case of the precipitation-enhanced detection compared to the label-free approach. The optimized method provided LOD of 100 CFU·mL–1 with linear range up to 106 CFU·mL–1. The total time of analysis including bacteria binding and enhancement step was below 60 min. The capability to analyze real samples with complex matrices was demonstrated on the detection of Salmonella in powdered milk. The developed sensor represents simple and robust approach for routine monitoring of food contamination.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA a Petr SKLÁDAL. Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk. Analytical Chemistry. Washington, DC: American Chemical Society, 2016, roč. 88, č. 23, s. 11830-11836. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.6b03511.

@article{1363884,
   author = {Farka, Zdeněk and Juřík, Tomáš and Pastucha, Matěj and Skládal, Petr},
   article_location = {Washington, DC},
   article_number = {23},
   doi = {http://dx.doi.org/10.1021/acs.analchem.6b03511},
   keywords = {Surface plasmon resonance biosensor; Biocatalyzed precipitation; Food safety; Bacterial pathogen; Salmonella Typhimurium; Atomic force microscopy},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk},
   url = {http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b03511},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1363884
AU  - Farka, Zdeněk - Juřík, Tomáš - Pastucha, Matěj - Skládal, Petr
PY  - 2016
TI  - Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk
JF  - Analytical Chemistry
VL  - 88
IS  - 23
SP  - 11830-11836
EP  - 11830-11836
PB  - American Chemical Society
SN  - 00032700
KW  - Surface plasmon resonance biosensor
KW  - Biocatalyzed precipitation
KW  - Food safety
KW  - Bacterial pathogen
KW  - Salmonella Typhimurium
KW  - Atomic force microscopy
UR  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b03511
L2  - http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b03511
N2  - Contamination of food by pathogenic bacteria has always been a serious threat for human health. The amount of food exports and imports has been increasing in recent years which requires precise food quality control with short analysis time and simplified sample treatment. Surface plasmon resonance (SPR) immunosensor enhanced by biocatalyzed precipitation was developed for the analysis of Salmonella in dairy products. The specific capture antibody was immobilized on the SPR chip which allowed a direct label-free detection of Salmonella Typhimurium with the limit of detection (LOD) of 104 CFU·mL–1 and the analysis time of 10 min. Alternatively, the secondary detection antibody was conjugated with horseradish peroxidase to provide a signal enhancement by the biocatalyzed conversion of 4-chloro-1-naphthol to insoluble benzo-4-chlorocyclohexadienone. The formation of precipitate was studied in detail by atomic force microscopy (AFM). The sensitivity was increased 40 times in case of the precipitation-enhanced detection compared to the label-free approach. The optimized method provided LOD of 100 CFU·mL–1 with linear range up to 106 CFU·mL–1. The total time of analysis including bacteria binding and enhancement step was below 60 min. The capability to analyze real samples with complex matrices was demonstrated on the detection of Salmonella in powdered milk. The developed sensor represents simple and robust approach for routine monitoring of food contamination.
ER  -

FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA a Petr SKLÁDAL. Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk. \textit{Analytical Chemistry}. Washington, DC: American Chemical Society, 2016, roč.~88, č.~23, s.~11830-11836. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.6b03511.

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