FARKA, Zdeněk, Tomáš JUŘÍK, Matěj PASTUCHA a Petr SKLÁDAL. Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk. Analytical Chemistry. Washington, DC: American Chemical Society, 2016, roč. 88, č. 23, s. 11830-11836. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.6b03511.
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Základní údaje
Originální název Enzymatic Precipitation Enhanced Surface Plasmon Resonance Immunosensor for the Detection of Salmonella in Powdered Milk
Autoři FARKA, Zdeněk (203 Česká republika, domácí), Tomáš JUŘÍK (203 Česká republika, domácí), Matěj PASTUCHA (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí).
Vydání Analytical Chemistry, Washington, DC, American Chemical Society, 2016, 0003-2700.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10406 Analytical chemistry
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 6.320
Kód RIV RIV/00216224:14740/16:00092189
Organizační jednotka Středoevropský technologický institut
Doi http://dx.doi.org/10.1021/acs.analchem.6b03511
UT WoS 000389556900080
Klíčová slova anglicky Surface plasmon resonance biosensor; Biocatalyzed precipitation; Food safety; Bacterial pathogen; Salmonella Typhimurium; Atomic force microscopy
Štítky rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnil: doc. Mgr. Zdeněk Farka, Ph.D., učo 357740. Změněno: 15. 9. 2018 18:50.
Anotace
Contamination of food by pathogenic bacteria has always been a serious threat for human health. The amount of food exports and imports has been increasing in recent years which requires precise food quality control with short analysis time and simplified sample treatment. Surface plasmon resonance (SPR) immunosensor enhanced by biocatalyzed precipitation was developed for the analysis of Salmonella in dairy products. The specific capture antibody was immobilized on the SPR chip which allowed a direct label-free detection of Salmonella Typhimurium with the limit of detection (LOD) of 104 CFU·mL–1 and the analysis time of 10 min. Alternatively, the secondary detection antibody was conjugated with horseradish peroxidase to provide a signal enhancement by the biocatalyzed conversion of 4-chloro-1-naphthol to insoluble benzo-4-chlorocyclohexadienone. The formation of precipitate was studied in detail by atomic force microscopy (AFM). The sensitivity was increased 40 times in case of the precipitation-enhanced detection compared to the label-free approach. The optimized method provided LOD of 100 CFU·mL–1 with linear range up to 106 CFU·mL–1. The total time of analysis including bacteria binding and enhancement step was below 60 min. The capability to analyze real samples with complex matrices was demonstrated on the detection of Salmonella in powdered milk. The developed sensor represents simple and robust approach for routine monitoring of food contamination.
Návaznosti
LQ1601, projekt VaVNázev: CEITEC 2020 (Akronym: CEITEC2020)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020
VytisknoutZobrazeno: 21. 6. 2024 06:52