OSBAK, Kara K., Simon HOUSTON, Karen V. LITHGOW, Conor J. MEEHAN, Michal STROUHAL, David ŠMAJS, Caroline E. CAMERON, Xaveer Van OSTADE, Chris R. KENYON a Geert A. Van RAEMDONCK. Characterizing the Syphilis-Causing Treponema pallidum ssp pallidum Proteome Using Complementary Mass Spectrometry. PLoS neglected tropical diseases, San Francisco: Public Library of Science, 2016, roč. 10, č. 9, s. 1-29. ISSN 1935-2735. doi:10.1371/journal.pntd.0004988.
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Základní údaje
Originální název Characterizing the Syphilis-Causing Treponema pallidum ssp pallidum Proteome Using Complementary Mass Spectrometry
Autoři OSBAK, Kara K. (56 Belgie), Simon HOUSTON (124 Kanada), Karen V. LITHGOW (124 Kanada), Conor J. MEEHAN (56 Belgie), Michal STROUHAL (203 Česká republika, domácí), David ŠMAJS (203 Česká republika, garant, domácí), Caroline E. CAMERON (124 Kanada), Xaveer Van OSTADE (56 Belgie), Chris R. KENYON (56 Belgie) a Geert A. Van RAEMDONCK (56 Belgie).
Vydání PLoS neglected tropical diseases, San Francisco, Public Library of Science, 2016, 1935-2735.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30300 3.3 Health sciences
Stát vydavatele Spojené státy americké
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 3.834
Kód RIV RIV/00216224:14110/16:00088426
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.1371/journal.pntd.0004988
UT WoS 000385627900043
Klíčová slova anglicky OUTER-MEMBRANE PROTEINS; PENICILLIN-BINDING PROTEIN; GRAM-NEGATIVE BACTERIA; GENOME-SCALE IDENTIFICATION; BARREL ASSEMBLY MACHINERY; SUBSP PALLIDUM; MOONLIGHTING PROTEINS; ANTIGENIC VARIATION; SIGNAL PEPTIDES; SUBCELLULAR-LOCALIZATION
Štítky EL OK
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Ing. Mgr. Věra Pospíšilíková, učo 9005. Změněno: 8. 12. 2016 14:35.
Anotace
Background The spirochete bacterium Treponema pallidum ssp. pallidum is the etiological agent of syphilis, a chronic multistage disease. Little is known about the global T. pallidum proteome, therefore mass spectrometry studies are needed to bring insights into pathogenicity and protein expression profiles during infection. Methodology/Principal Findings To better understand the T. pallidum proteome profile during infection, we studied T. pallidum ssp. pallidum DAL-1 strain bacteria isolated from rabbits using complementary mass spectrometry techniques, including multidimensional peptide separation and protein identification via matrix-assisted laser desorption ionization-time of flight (MALDI-TOF/TOF) and electrospray ionization (ESI-LTQ-Orbitrap) tandem mass spectrometry. A total of 6033 peptides were detected, corresponding to 557 unique T. pallidum proteins at a high level of confidence, representing 54% of the predicted proteome. A previous gel-based T. pallidum MS proteome study detected 58 of these proteins. One hundred fourteen of the detected proteins were previously annotated as hypothetical or uncharacterized proteins; this is the first account of 106 of these proteins at the protein level. Detected proteins were characterized according to their predicted biological function and localization; half were allocated into a wide range of functional categories. Proteins annotated as potential membrane proteins and proteins with unclear functional annotations were subjected to an additional bioinformatics pipeline analysis to facilitate further characterization. A total of 116 potential membrane proteins were identified, of which 16 have evidence supporting outer membrane localization. We found 8/12 proteins related to the paralogous tpr gene family: TprB, TprC/D, TprE, TprG, TprH, TprI and TprJ. Protein abundance was semi-quantified using label-free spectral counting methods. A low correlation (r = 0.26) was found between previous microarray signal data and protein abundance. Conclusions This is the most comprehensive description of the global T. pallidum proteome to date. These data provide valuable insights into in vivo T. pallidum protein expression, paving the way for improved understanding of the pathogenicity of this enigmatic organism.
Návaznosti
GAP302/12/0574, projekt VaVNázev: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema
Investor: Grantová agentura ČR, Standardní projekty
GP14-29596P, projekt VaVNázev: Genomová variabilita treponem během experimentální infekce
Investor: Grantová agentura ČR, Postdoktorské projekty
VytisknoutZobrazeno: 13. 12. 2019 07:33