All-in-one detector of circulating mRNA based on a smartphone

D 2016

All-in-one detector of circulating mRNA based on a smartphone

CMIEL, Vratislav, Jaromír GUMULEC, Ondrej SVOBODA, Martina RAUDENSKÁ, Kristýna HUDCOVÁ et. al.

Základní údaje

Originální název

All-in-one detector of circulating mRNA based on a smartphone

Autoři

CMIEL, Vratislav (203 Česká republika), Jaromír GUMULEC (203 Česká republika, domácí), Ondrej SVOBODA (203 Česká republika), Martina RAUDENSKÁ (203 Česká republika, domácí), Kristýna HUDCOVÁ (203 Česká republika, domácí), Jiri SEKORA (203 Česká republika), Jaroslav BALOGH (203 Česká republika), Michal MASAŘÍK (203 Česká republika, garant, domácí) a Ivo PROVAZNIK (203 Česká republika)

Vydání

Bellingham, Proceedings of SPIE : Optical Diagnostics and Sensing XVI - Toward Point-of-Care Diagnostics, od s. 1-6, 6 s. 2016

Nakladatel

SPIE

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Kód RIV

RIV/00216224:14110/16:00092235

Organizační jednotka

Lékařská fakulta

ISBN

978-1-62841-949-8

ISSN

DOI

http://dx.doi.org/10.1117/12.2213253

UT WoS

000379998400006

Klíčová slova anglicky

mRNA; smartphone; PCR; cancer; metallothionein

Štítky

EL OK

Změněno: 26. 4. 2017 12:25, Soňa Böhmová

Anotace

V originále

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

Citovat

CMIEL, Vratislav, Jaromír GUMULEC, Ondrej SVOBODA, Martina RAUDENSKÁ, Kristýna HUDCOVÁ, Jiri SEKORA, Jaroslav BALOGH, Michal MASAŘÍK a Ivo PROVAZNIK. All-in-one detector of circulating mRNA based on a smartphone. In Gerard L. Coté. Proceedings of SPIE : Optical Diagnostics and Sensing XVI - Toward Point-of-Care Diagnostics. Bellingham: SPIE, 2016, s. 1-6. ISBN 978-1-62841-949-8. Dostupné z: https://dx.doi.org/10.1117/12.2213253.

@inproceedings{1364184,
   author = {Cmiel, Vratislav and Gumulec, Jaromír and Svoboda, Ondrej and Raudenská, Martina and Hudcová, Kristýna and Sekora, Jiri and Balogh, Jaroslav and Masařík, Michal and Provaznik, Ivo},
   address = {Bellingham},
   booktitle = {Proceedings of SPIE : Optical Diagnostics and Sensing XVI - Toward Point-of-Care Diagnostics},
   doi = {http://dx.doi.org/10.1117/12.2213253},
   editor = {Gerard L. Coté},
   keywords = {mRNA; smartphone; PCR; cancer; metallothionein},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Bellingham},
   isbn = {978-1-62841-949-8},
   pages = {1-6},
   publisher = {SPIE},
   title = {All-in-one detector of circulating mRNA based on a smartphone},
   year = {2016}
}

TY  - JOUR
ID  - 1364184
AU  - Cmiel, Vratislav - Gumulec, Jaromír - Svoboda, Ondrej - Raudenská, Martina - Hudcová, Kristýna - Sekora, Jiri - Balogh, Jaroslav - Masařík, Michal - Provaznik, Ivo
PY  - 2016
TI  - All-in-one detector of circulating mRNA based on a smartphone
PB  - SPIE
CY  - Bellingham
SN  - 9781628419498
KW  - mRNA
KW  - smartphone
KW  - PCR
KW  - cancer
KW  - metallothionein
N2  - Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.
ER  -

CMIEL, Vratislav, Jaromír GUMULEC, Ondrej SVOBODA, Martina RAUDENSKÁ, Kristýna HUDCOVÁ, Jiri SEKORA, Jaroslav BALOGH, Michal MASAŘÍK a Ivo PROVAZNIK. All-in-one detector of circulating mRNA based on a smartphone. In Gerard L. Coté. \textit{Proceedings of SPIE : Optical Diagnostics and Sensing XVI - Toward Point-of-Care Diagnostics}. Bellingham: SPIE, 2016, s.~1-6. ISBN~978-1-62841-949-8. Dostupné z: https://dx.doi.org/10.1117/12.2213253.

Podrobný výpis o publikaci