The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate
Cancer Therapy

J 2016

The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy

MILOSAVLJEVIC, Vedran; Yazan HADDAD; Miguel Angel Merlos RODRIGO; Amitava MOULICK; Hana POLANSKÁ et. al.

Basic information

Original name

The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy

Authors

MILOSAVLJEVIC, Vedran (203 Czech Republic); Yazan HADDAD (203 Czech Republic); Miguel Angel Merlos RODRIGO (203 Czech Republic); Amitava MOULICK (203 Czech Republic); Hana POLANSKÁ (203 Czech Republic, guarantor, belonging to the institution); David HYNEK (203 Czech Republic); Zbynek HEGER (203 Czech Republic); Pavel KOPEL (203 Czech Republic) and Vojtech ADAM (203 Czech Republic)

Edition

Plos one, San Francisco, Public Library of Science, 2016, 1932-6203

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 2.806

RIV identification code

RIV/00216224:14110/16:00092236

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.1371/journal.pone.0163983

UT WoS

000385504400015

EID Scopus

2-s2.0-84991498330

Keywords in English

TRANSCRIPTION FACTOR SP1; STEADY-STATE; CELL-LINES; EXPRESSION; APOPTOSIS; ACTIVATION; MICROARRAY; PROTEIN; MODEL; P53

Abstract

In the original language

Prostate cancer cells control energy metabolism by chelating intracellular zinc. Thus, zinc delivery has been a popular therapeutic approach for prostate cancer. Here, we propose the use of the membrane-penetrating peptide Novicidin connected to zinc-Schiff base as a carrier vehicle for the delivery of zinc to prostate cells. Mass spectrometry, electrochemistry and spectrophotometry confirmed the formation/stability of this complex and provided insight regarding the availability of zinc for complex interactions. This delivery system showed minor toxicity in normal PNT1A cells and high potency towards PC3 tumor cells. The complex preferentially penetrated PC3 tumor cells in contrast to confinement to the membranes of PNT1A. Furthermore, zinc uptake was confirmed in both cell lines. Molecular analysis was used to confirm the activation of zinc stress (e.g., ZnT-1) and apoptosis (e.g., CASP-1). Our results strongly suggest that the zinc-Schiff base-Novicidin complex has great potential as a novel anticancer drug.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Cite

MILOSAVLJEVIC, Vedran; Yazan HADDAD; Miguel Angel Merlos RODRIGO; Amitava MOULICK; Hana POLANSKÁ; David HYNEK; Zbynek HEGER; Pavel KOPEL and Vojtech ADAM. The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy. Plos one. San Francisco: Public Library of Science, 2016, vol. 11, No 10, p. 1-19. ISSN 1932-6203. Available from: https://doi.org/10.1371/journal.pone.0163983.

@article{1364186,
   author = {Milosavljevic, Vedran and Haddad, Yazan and Rodrigo, Miguel Angel Merlos and Moulick, Amitava and Polanská, Hana and Hynek, David and Heger, Zbynek and Kopel, Pavel and Adam, Vojtech},
   article_location = {San Francisco},
   article_number = {10},
   doi = {https://doi.org/10.1371/journal.pone.0163983},
   keywords = {TRANSCRIPTION FACTOR SP1; STEADY-STATE; CELL-LINES; EXPRESSION; APOPTOSIS; ACTIVATION; MICROARRAY; PROTEIN; MODEL; P53},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy},
   volume = {11},
   year = {2016}
}

TY  - JOUR
ID  - 1364186
AU  - Milosavljevic, Vedran - Haddad, Yazan - Rodrigo, Miguel Angel Merlos - Moulick, Amitava - Polanská, Hana - Hynek, David - Heger, Zbynek - Kopel, Pavel - Adam, Vojtech
PY  - 2016
TI  - The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy
JF  - Plos one
VL  - 11
IS  - 10
SP  - 1-19
EP  - 1-19
PB  - Public Library of Science
SN  - 19326203
KW  - TRANSCRIPTION FACTOR SP1
KW  - STEADY-STATE
KW  - CELL-LINES
KW  - EXPRESSION
KW  - APOPTOSIS
KW  - ACTIVATION
KW  - MICROARRAY
KW  - PROTEIN
KW  - MODEL
KW  - P53
N2  - Prostate cancer cells control energy metabolism by chelating intracellular zinc. Thus, zinc delivery has been a popular therapeutic approach for prostate cancer. Here, we propose the use of the membrane-penetrating peptide Novicidin connected to zinc-Schiff base as a carrier vehicle for the delivery of zinc to prostate cells. Mass spectrometry, electrochemistry and spectrophotometry confirmed the formation/stability of this complex and provided insight regarding the availability of zinc for complex interactions. This delivery system showed minor toxicity in normal PNT1A cells and high potency towards PC3 tumor cells. The complex preferentially penetrated PC3 tumor cells in contrast to confinement to the membranes of PNT1A. Furthermore, zinc uptake was confirmed in both cell lines. Molecular analysis was used to confirm the activation of zinc stress (e.g., ZnT-1) and apoptosis (e.g., CASP-1). Our results strongly suggest that the zinc-Schiff base-Novicidin complex has great potential as a novel anticancer drug.
ER  -

MILOSAVLJEVIC, Vedran; Yazan HADDAD; Miguel Angel Merlos RODRIGO; Amitava MOULICK; Hana POLANSKÁ; David HYNEK; Zbynek HEGER; Pavel KOPEL and Vojtech ADAM. The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy. \textit{Plos one}. San Francisco: Public Library of Science, 2016, vol.~11, No~10, p.~1-19. ISSN~1932-6203. Available from: https://doi.org/10.1371/journal.pone.0163983.

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