Co-delivery of VP-16 and Bcl-2-targeted antisense on
PEG-grafted oMWCNTs for synergistic in vitro anti-cancer
effects in non-small and small cell lung cancer

J 2017

Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer

HEGER, Zbynek, Hana POLANSKÁ, Sona KRIZKOVA, Jan BALVAN, Martina RAUDENSKÁ et. al.

Základní údaje

Originální název

Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer

Autoři

HEGER, Zbynek (203 Česká republika), Hana POLANSKÁ (203 Česká republika, domácí), Sona KRIZKOVA (203 Česká republika), Jan BALVAN (203 Česká republika, domácí), Martina RAUDENSKÁ (203 Česká republika, domácí), Simona DOSTALOVA (203 Česká republika), Amitava MOULICK (203 Česká republika), Michal MASAŘÍK (203 Česká republika, garant, domácí) a Vojtech ADAM (203 Česká republika)

Vydání

Colloids and Surfaces B: Biointerfaces, Amsterdam, Elsevier, 2017, 0927-7765

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.997

Kód RIV

RIV/00216224:14110/17:00095967

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.colsurfb.2016.11.023

UT WoS

000393726900016

Klíčová slova anglicky

Drug delivery; Etoposide; In vitro release; Multi-walled carbon nanotubes; Nanomedicine

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2018 16:24, Soňa Böhmová

Anotace

V originále

Present study describes the preparation of a polyethylene glycol-grafted oxidized multi-walled carbon nanotubes (oMWCNTs-PEG) hybrid nanosystem as a carrier of etoposide (VP-16) and Bcl-2 phosphorothioate antisense deoxyoligonucleotides (Aso) to achieve a superior cytostastic efficacy in non-small and small cell lung cancer in vitro. We have demonstrated that the adsorption of hydrophobic VP-16 and Bcl-2 Aso results in a stable nanotransporter exhibiting good dispersion with excellent release profiles (both, in pH 7.4 and 4.8) and negligible hemolytic activity (up to 6.5%). The evaluation of cytotoxicity was carried out in in vitro using small cell (SCLC; DMS53) and non-small cell lung cancer (NSCLC; NCIH2135) cell lines. It was found that Bcl-2 interference significantly increased the anti-cancer efficiency of VP-16 in the chemoresistant NSCLC cells. This was further supported using a flow-cytometry (Annexin V/propidium iodide assay), which revealed a significant increase in apoptotic cells in both the cell lines after the co-administration of VP-16 and Bcl-2 Aso using oMWCNTs-PEG hybrid, and fluorescence microscopy, which showed an increase in reactive oxygen species identified after Bcl-2 knock-down. Overall, oMWCNTs-PEG provided an exceptional biocompatible vehicle enabling the internalization of negatively charged nucleic acids and pH-sensitive release of cargoes in a hypoxic environment of the most of solid tumors. Moreover, Aso specifically binding to the first six codons of the Bcl-2 mRNA gave a satisfactorily decrease in Bcl-2 translation and an increase in NCIH2135 chemosensitivity towards VP-16.

Citovat

HEGER, Zbynek, Hana POLANSKÁ, Sona KRIZKOVA, Jan BALVAN, Martina RAUDENSKÁ, Simona DOSTALOVA, Amitava MOULICK, Michal MASAŘÍK a Vojtech ADAM. Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer. Colloids and Surfaces B: Biointerfaces. Amsterdam: Elsevier, 2017, roč. 150, 1 February 2017, s. 131-140. ISSN 0927-7765. Dostupné z: https://dx.doi.org/10.1016/j.colsurfb.2016.11.023.

@article{1364900,
   author = {Heger, Zbynek and Polanská, Hana and Krizkova, Sona and Balvan, Jan and Raudenská, Martina and Dostalova, Simona and Moulick, Amitava and Masařík, Michal and Adam, Vojtech},
   article_location = {Amsterdam},
   article_number = {1 February 2017},
   doi = {http://dx.doi.org/10.1016/j.colsurfb.2016.11.023},
   keywords = {Drug delivery; Etoposide; In vitro release; Multi-walled carbon nanotubes; Nanomedicine},
   language = {eng},
   issn = {0927-7765},
   journal = {Colloids and Surfaces B: Biointerfaces},
   title = {Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer},
   volume = {150},
   year = {2017}
}

TY  - JOUR
ID  - 1364900
AU  - Heger, Zbynek - Polanská, Hana - Krizkova, Sona - Balvan, Jan - Raudenská, Martina - Dostalova, Simona - Moulick, Amitava - Masařík, Michal - Adam, Vojtech
PY  - 2017
TI  - Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer
JF  - Colloids and Surfaces B: Biointerfaces
VL  - 150
IS  - 1 February 2017
SP  - 131-140
EP  - 131-140
PB  - Elsevier
SN  - 09277765
KW  - Drug delivery
KW  - Etoposide
KW  - In vitro release
KW  - Multi-walled carbon nanotubes
KW  - Nanomedicine
N2  - Present study describes the preparation of a polyethylene glycol-grafted oxidized multi-walled carbon nanotubes (oMWCNTs-PEG) hybrid nanosystem as a carrier of etoposide (VP-16) and Bcl-2 phosphorothioate antisense deoxyoligonucleotides (Aso) to achieve a superior cytostastic efficacy in non-small and small cell lung cancer in vitro. We have demonstrated that the adsorption of hydrophobic VP-16 and Bcl-2 Aso results in a stable nanotransporter exhibiting good dispersion with excellent release profiles (both, in pH 7.4 and 4.8) and negligible hemolytic activity (up to 6.5%). The evaluation of cytotoxicity was carried out in in vitro using small cell (SCLC; DMS53) and non-small cell lung cancer (NSCLC; NCIH2135) cell lines. It was found that Bcl-2 interference significantly increased the anti-cancer efficiency of VP-16 in the chemoresistant NSCLC cells. This was further supported using a flow-cytometry (Annexin V/propidium iodide assay), which revealed a significant increase in apoptotic cells in both the cell lines after the co-administration of VP-16 and Bcl-2 Aso using oMWCNTs-PEG hybrid, and fluorescence microscopy, which showed an increase in reactive oxygen species identified after Bcl-2 knock-down. Overall, oMWCNTs-PEG provided an exceptional biocompatible vehicle enabling the internalization of negatively charged nucleic acids and pH-sensitive release of cargoes in a hypoxic environment of the most of solid tumors. Moreover, Aso specifically binding to the first six codons of the Bcl-2 mRNA gave a satisfactorily decrease in Bcl-2 translation and an increase in NCIH2135 chemosensitivity towards VP-16.
ER  -

HEGER, Zbynek, Hana POLANSKÁ, Sona KRIZKOVA, Jan BALVAN, Martina RAUDENSKÁ, Simona DOSTALOVA, Amitava MOULICK, Michal MASAŘÍK a Vojtech ADAM. Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer. \textit{Colloids and Surfaces B: Biointerfaces}. Amsterdam: Elsevier, 2017, roč.~150, 1 February 2017, s.~131-140. ISSN~0927-7765. Dostupné z: https://dx.doi.org/10.1016/j.colsurfb.2016.11.023.

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