Absence of positive selection on CenH3 in Luzula suggests that
holokinetic chromosomes may suppress centromere drive

J 2016

Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive

ZEDEK, František a Petr BUREŠ

Základní údaje

Originální název

Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive

Autoři

ZEDEK, František (203 Česká republika, domácí) a Petr BUREŠ (203 Česká republika, garant, domácí)

Vydání

Annals of Botany, Oxford, Oxford University Press, 2016, 0305-7364

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.041

Kód RIV

RIV/00216224:14310/16:00088443

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1093/aob/mcw186

UT WoS

000399546800015

Klíčová slova anglicky

centromere drive; meiotic drive; holokinetic chromosomes; holocentric chromosomes; positive selection; adaptive evolution; Centromeric histone H3; CenH3; CENP-A; holokinetic drive

Štítky

AKR

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 3. 2018 16:15, prof. RNDr. Petr Bureš, Ph.D.

Anotace

V originále

Background and Aims: The centromere drive theory explains diversity of eukaryotic centromeres as a consequence of the recurrent conflict between centromeric repeats and centromeric histone H3 (CenH3), in which selfish centromeres exploit meiotic asymmetry and CenH3 evolves adaptively to counterbalance deleterious consequences of driving centromeres. Accordingly, adaptively evolving CenH3 has so far been observed only in eukaryotes with asymmetric meiosis. However, if such evolution is a consequence of centromere drive, it should depend not only on meiotic asymmetry but also on monocentric or holokinetic chromosomal structure. Selective pressures acting on CenH3 have never been investigated in organisms with holokinetic meiosis despite the fact that holokinetic chromosomes have been hypothesized to suppress centromere drive. Therefore, the present study evaluates selective pressures acting on the CenH3 gene in holokinetic organisms for the first time, specifically in the representatives of the plant genus Luzula (Juncaceae), in which the kinetochore formation is not co-localized with any type of centromeric repeat. Methods: PCR, cloning and sequencing, and database searches were used to obtain coding CenH3 sequences from Luzula species. Codon substitution models were employed to infer selective regimes acting on CenH3 in Luzula. Key Results: In addition to the two previously published CenH3 sequences from L. nivea, 16 new CenH3 sequences have been isolated from 12 Luzula species. Two CenH3 isoforms in Luzula that originated by a duplication event prior to the divergence of analysed species were found. No signs of positive selection acting on CenH3 in Luzula were detected. Instead, evidence was found that selection on CenH3 of Luzula might have been relaxed. Conclusions: The results indicate that holokinetism itself may suppress centromere drive and, therefore, holokinetic chromosomes might have evolved as a defence against centromere drive.

Návaznosti

GA13-29362S, projekt VaV

Název: Evoluce holocentrických chromosomů (Akronym: EvoHolo)

Investor: Grantová agentura ČR, Evoluce holocentrických chromosomů

Citovat

ZEDEK, František a Petr BUREŠ. Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive. Annals of Botany. Oxford: Oxford University Press, 2016, roč. 118, č. 7, s. 1347-1352. ISSN 0305-7364. Dostupné z: https://dx.doi.org/10.1093/aob/mcw186.

@article{1364913,
   author = {Zedek, František and Bureš, Petr},
   article_location = {Oxford},
   article_number = {7},
   doi = {http://dx.doi.org/10.1093/aob/mcw186},
   keywords = {centromere drive; meiotic drive; holokinetic chromosomes; holocentric chromosomes; positive selection; adaptive evolution; Centromeric histone H3; CenH3; CENP-A; holokinetic drive},
   language = {eng},
   issn = {0305-7364},
   journal = {Annals of Botany},
   title = {Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive},
   url = {http://aob.oxfordjournals.org/content/118/7/1347.abstract},
   volume = {118},
   year = {2016}
}

TY  - JOUR
ID  - 1364913
AU  - Zedek, František - Bureš, Petr
PY  - 2016
TI  - Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive
JF  - Annals of Botany
VL  - 118
IS  - 7
SP  - 1347-1352
EP  - 1347-1352
PB  - Oxford University Press
SN  - 03057364
KW  - centromere drive
KW  - meiotic drive
KW  - holokinetic chromosomes
KW  - holocentric chromosomes
KW  - positive selection
KW  - adaptive evolution
KW  - Centromeric histone H3
KW  - CenH3
KW  - CENP-A
KW  - holokinetic drive
UR  - http://aob.oxfordjournals.org/content/118/7/1347.abstract
L2  - http://aob.oxfordjournals.org/content/118/7/1347.abstract
N2  - Background and Aims: The centromere drive theory explains diversity of eukaryotic centromeres as a consequence of the recurrent conflict between centromeric repeats and centromeric histone H3 (CenH3), in which selfish centromeres exploit meiotic asymmetry and CenH3 evolves adaptively to counterbalance deleterious consequences of driving centromeres. Accordingly, adaptively evolving CenH3 has so far been observed only in eukaryotes with asymmetric meiosis. However, if such evolution is a consequence of centromere drive, it should depend not only on meiotic asymmetry but also on monocentric or holokinetic chromosomal structure. Selective pressures acting on CenH3 have never been investigated in organisms with holokinetic meiosis despite the fact that holokinetic chromosomes have been hypothesized to suppress centromere drive. Therefore, the present study evaluates selective pressures acting on the CenH3 gene in holokinetic organisms for the first time, specifically in the representatives of the plant genus Luzula (Juncaceae), in which the kinetochore formation is not co-localized with any type of centromeric repeat. Methods: PCR, cloning and sequencing, and database searches were used to obtain coding CenH3 sequences from Luzula species. Codon substitution models were employed to infer selective regimes acting on CenH3 in Luzula. Key Results: In addition to the two previously published CenH3 sequences from L. nivea, 16 new CenH3 sequences have been isolated from 12 Luzula species. Two CenH3 isoforms in Luzula that originated by a duplication event prior to the divergence of analysed species were found. No signs of positive selection acting on CenH3 in Luzula were detected. Instead, evidence was found that selection on CenH3 of Luzula might have been relaxed. Conclusions: The results indicate that holokinetism itself may suppress centromere drive and, therefore, holokinetic chromosomes might have evolved as a defence against centromere drive.
ER  -

ZEDEK, František a Petr BUREŠ. Absence of positive selection on CenH3 in Luzula suggests that holokinetic chromosomes may suppress centromere drive. \textit{Annals of Botany}. Oxford: Oxford University Press, 2016, roč.~118, č.~7, s.~1347-1352. ISSN~0305-7364. Dostupné z: https://dx.doi.org/10.1093/aob/mcw186.

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