PRDM9 interactions with other proteins provide a link between
recombination hotspots and the chromosomal axis in meiosis

J 2017

PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis

PARVANOV, Emil Damyanov, Hui TIAN, Timothy BILLINGS, Ruth L. SAXL, Catrina SPRUCE et. al.

Základní údaje

Originální název

PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis

Autoři

PARVANOV, Emil Damyanov (100 Bulharsko, domácí), Hui TIAN (840 Spojené státy), Timothy BILLINGS (840 Spojené státy), Ruth L. SAXL (840 Spojené státy), Catrina SPRUCE (840 Spojené státy), Rakesh AITHAL (356 Indie, domácí), Lumír KREJČÍ (203 Česká republika, garant, domácí), Kenneth PAIGEN (840 Spojené státy) a Petko M. PETKOV (840 Spojené státy)

Vydání

Molecular Biology of the Cell, Bethesda, American Society for Cell Biology, 2017, 1059-1524

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.512

Kód RIV

RIV/00216224:14110/17:00094599

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1091/mbc.E16-09-0686

UT WoS

000394268700014

Klíčová slova anglicky

CXXC FINGER PROTEIN-1; MEIOTIC RECOMBINATION; HISTONE METHYLTRANSFERASES; CHROMATIN MODIFICATIONS; BIOLOGICAL FUNCTIONS; GENE REARRANGEMENTS; ANALYSIS REVEALS; EWING SARCOMA; HOT-SPOTS; METHYLATION

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 3. 2018 15:40, Soňa Böhmová

Anotace

V originále

In mammals, meiotic recombination occurs at 1-2 kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We now show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and co-immunoprecipitation from mouse spermatocytes, we identified four proteins that directly interact with PRDM9's KRAB domain, namely CXXC1, EWSR1, EHMT2, and CDYL. These proteins are co-expressed in spermatocytes at the early stages of meiotic prophase I, the limited period when PRDM9 is expressed. We also detected association of PRDM9-bound complexes with the meiotic cohesin REC8 and the synaptonemal complex proteins SYCP3 and SYCP1. Our results suggest a model in which PRDM9-bound hotspot DNA is brought to the chromosomal axis by the action of these proteins, ensuring the proper chromatin and spatial environment for subsequent recombination events.

Návaznosti

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

2SGA2773, interní kód MU

Název: Prdm9 - linking the histone modifications and recombination (Akronym: PLHMR)

Investor: Jihomoravský kraj, Prdm9 - linking the histone modifications and recombination, Granty pro zahraniční vědce

Citovat

PARVANOV, Emil Damyanov, Hui TIAN, Timothy BILLINGS, Ruth L. SAXL, Catrina SPRUCE, Rakesh AITHAL, Lumír KREJČÍ, Kenneth PAIGEN a Petko M. PETKOV. PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis. Molecular Biology of the Cell. Bethesda: American Society for Cell Biology, 2017, roč. 28, č. 3, s. 488-499. ISSN 1059-1524. Dostupné z: https://dx.doi.org/10.1091/mbc.E16-09-0686.

@article{1367105,
   author = {Parvanov, Emil Damyanov and Tian, Hui and Billings, Timothy and Saxl, Ruth L. and Spruce, Catrina and Aithal, Rakesh and Krejčí, Lumír and Paigen, Kenneth and Petkov, Petko M.},
   article_location = {Bethesda},
   article_number = {3},
   doi = {http://dx.doi.org/10.1091/mbc.E16-09-0686},
   keywords = {CXXC FINGER PROTEIN-1; MEIOTIC RECOMBINATION; HISTONE METHYLTRANSFERASES; CHROMATIN MODIFICATIONS; BIOLOGICAL FUNCTIONS; GENE REARRANGEMENTS; ANALYSIS REVEALS; EWING SARCOMA; HOT-SPOTS; METHYLATION},
   language = {eng},
   issn = {1059-1524},
   journal = {Molecular Biology of the Cell},
   title = {PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis},
   volume = {28},
   year = {2017}
}

TY  - JOUR
ID  - 1367105
AU  - Parvanov, Emil Damyanov - Tian, Hui - Billings, Timothy - Saxl, Ruth L. - Spruce, Catrina - Aithal, Rakesh - Krejčí, Lumír - Paigen, Kenneth - Petkov, Petko M.
PY  - 2017
TI  - PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis
JF  - Molecular Biology of the Cell
VL  - 28
IS  - 3
SP  - 488-499
EP  - 488-499
PB  - American Society for Cell Biology
SN  - 10591524
KW  - CXXC FINGER PROTEIN-1
KW  - MEIOTIC RECOMBINATION
KW  - HISTONE METHYLTRANSFERASES
KW  - CHROMATIN MODIFICATIONS
KW  - BIOLOGICAL FUNCTIONS
KW  - GENE REARRANGEMENTS
KW  - ANALYSIS REVEALS
KW  - EWING SARCOMA
KW  - HOT-SPOTS
KW  - METHYLATION
N2  - In mammals, meiotic recombination occurs at 1-2 kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We now show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and co-immunoprecipitation from mouse spermatocytes, we identified four proteins that directly interact with PRDM9's KRAB domain, namely CXXC1, EWSR1, EHMT2, and CDYL. These proteins are co-expressed in spermatocytes at the early stages of meiotic prophase I, the limited period when PRDM9 is expressed. We also detected association of PRDM9-bound complexes with the meiotic cohesin REC8 and the synaptonemal complex proteins SYCP3 and SYCP1. Our results suggest a model in which PRDM9-bound hotspot DNA is brought to the chromosomal axis by the action of these proteins, ensuring the proper chromatin and spatial environment for subsequent recombination events.
ER  -

PARVANOV, Emil Damyanov, Hui TIAN, Timothy BILLINGS, Ruth L. SAXL, Catrina SPRUCE, Rakesh AITHAL, Lumír KREJČÍ, Kenneth PAIGEN a Petko M. PETKOV. PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis. \textit{Molecular Biology of the Cell}. Bethesda: American Society for Cell Biology, 2017, roč.~28, č.~3, s.~488-499. ISSN~1059-1524. Dostupné z: https://dx.doi.org/10.1091/mbc.E16-09-0686.

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