Doxorubicin and Liposomal Doxorubicin Differentially Affect
Expression of miR-208a and let-7g in Rat Ventricles and Atria

J 2017

Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria

NOVÁK, Jan, Jiří ŠÁNA, Tibor STRAČINA, Marie NOVÁKOVÁ, Ondřej SLABÝ et. al.

Základní údaje

Originální název

Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria

Autoři

NOVÁK, Jan (203 Česká republika, garant, domácí), Jiří ŠÁNA (203 Česká republika, domácí), Tibor STRAČINA (703 Slovensko, domácí), Marie NOVÁKOVÁ (203 Česká republika, domácí) a Ondřej SLABÝ (203 Česká republika, domácí)

Vydání

Cardiovascular Toxicology, Totowa, Humana Press, 2017, 1530-7905

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.989

Kód RIV

RIV/00216224:14110/17:00096001

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s12012-016-9393-8

UT WoS

000404172500014

Klíčová slova anglicky

Cardiotoxicity; Doxorubicin; let-7g; lipodox; microRNAs; miR-208a

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2018 16:35, Soňa Böhmová

Anotace

V originále

Anthracyclines use is limited by profound cardiotoxicity. Involvement of miRNAs in anthracycline-induced cardiotoxicity (AIC) is still not completely understood. Thus, the expression of AIC-related microRNAs was determined in rat atria and ventricles after doxorubicin (DOX) and liposomal doxorubicin (L-DOX) administration. Vehiculum, DOX or L-DOX were applied intraperitoneally in a single dose to male Wistar rats (3 groups: control, DOX and L-DOX, respectively). Rats were sacrificed after 24 h, and samples from left atrium (LA)/ventricle (LV) and right atrium (RA)/ventricle (RV) were obtained. Expressions of miR-208a, let-7g and snU6 were determined using qRT-PCR. In the control group, miR-208a was highly abundant in the atria compared to the ventricles and in the left-sided structures compared to the right-sided structures, while let-7g showed only atrio-ventricular gradient with predominant expression in the atria. Administration of both DOX and L-DOX resulted in 38.87 and 23.57% reduction in miR-208a expression in the LV (p = 0.028) and in 13.79 and 14.70% reduction in let-7g expression in the LA (p = 0.015), respectively. Acute administration of DOX/L-DOX alters expression of miR-208a in LV and of let-7g in LA. These changes may partly contribute to the development of AIC.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

MUNI/A/1326/2014, interní kód MU

Název: Kardiovaskulární systém od buňky k lůžku pacienta (Akronym: KASBUNPAC)

Investor: Masarykova univerzita, Kardiovaskulární systém od buňky k lůžku pacienta, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

MUNI/A/1365/2015, interní kód MU

Název: Kardiovaskulární systém: od modelu přes terapii k prevenci (Akronym: KAMOTEPRE)

Investor: Masarykova univerzita, Kardiovaskulární systém: od modelu přes terapii k prevenci, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Přiložené soubory

EL_1367107.pdf

Požádat o autorskou verzi souboru

Citovat

NOVÁK, Jan, Jiří ŠÁNA, Tibor STRAČINA, Marie NOVÁKOVÁ a Ondřej SLABÝ. Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria. Cardiovascular Toxicology. Totowa: Humana Press, 2017, roč. 17, č. 3, s. 355-359. ISSN 1530-7905. Dostupné z: https://dx.doi.org/10.1007/s12012-016-9393-8.

@article{1367107,
   author = {Novák, Jan and Šána, Jiří and Stračina, Tibor and Nováková, Marie and Slabý, Ondřej},
   article_location = {Totowa},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s12012-016-9393-8},
   keywords = {Cardiotoxicity; Doxorubicin; let-7g; lipodox; microRNAs; miR-208a},
   language = {eng},
   issn = {1530-7905},
   journal = {Cardiovascular Toxicology},
   title = {Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria},
   volume = {17},
   year = {2017}
}

TY  - JOUR
ID  - 1367107
AU  - Novák, Jan - Šána, Jiří - Stračina, Tibor - Nováková, Marie - Slabý, Ondřej
PY  - 2017
TI  - Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria
JF  - Cardiovascular Toxicology
VL  - 17
IS  - 3
SP  - 355-359
EP  - 355-359
PB  - Humana Press
SN  - 15307905
KW  - Cardiotoxicity
KW  - Doxorubicin
KW  - let-7g
KW  - lipodox
KW  - microRNAs
KW  - miR-208a
N2  - Anthracyclines use is limited by profound cardiotoxicity. Involvement of miRNAs in anthracycline-induced cardiotoxicity (AIC) is still not completely understood. Thus, the expression of AIC-related microRNAs was determined in rat atria and ventricles after doxorubicin (DOX) and liposomal doxorubicin (L-DOX) administration. Vehiculum, DOX or L-DOX were applied intraperitoneally in a single dose to male Wistar rats (3 groups: control, DOX and L-DOX, respectively). Rats were sacrificed after 24 h, and samples from left atrium (LA)/ventricle (LV) and right atrium (RA)/ventricle (RV) were obtained. Expressions of miR-208a, let-7g and snU6 were determined using qRT-PCR. In the control group, miR-208a was highly abundant in the atria compared to the ventricles and in the left-sided structures compared to the right-sided structures, while let-7g showed only atrio-ventricular gradient with predominant expression in the atria. Administration of both DOX and L-DOX resulted in 38.87 and 23.57% reduction in miR-208a expression in the LV (p = 0.028) and in 13.79 and 14.70% reduction in let-7g expression in the LA (p = 0.015), respectively. Acute administration of DOX/L-DOX alters expression of miR-208a in LV and of let-7g in LA. These changes may partly contribute to the development of AIC.
ER  -

NOVÁK, Jan, Jiří ŠÁNA, Tibor STRAČINA, Marie NOVÁKOVÁ a Ondřej SLABÝ. Doxorubicin and Liposomal Doxorubicin Differentially Affect Expression of miR-208a and let-7g in Rat Ventricles and Atria. \textit{Cardiovascular Toxicology}. Totowa: Humana Press, 2017, roč.~17, č.~3, s.~355-359. ISSN~1530-7905. Dostupné z: https://dx.doi.org/10.1007/s12012-016-9393-8.

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