A Polar and Nucleotide-Dependent Mechanism of Action for RAD51
Paralogs in RAD51 Filament Remodeling

J 2016

A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling

TAYLOR, Martin R.G., Mário ŠPÍREK, Chu Jian MA, Raffaella CARZANIGA, Tohru TAKAKI et. al.

Základní údaje

Originální název

A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling

Autoři

TAYLOR, Martin R.G. (826 Velká Británie a Severní Irsko), Mário ŠPÍREK (703 Slovensko, domácí), Chu Jian MA (840 Spojené státy), Raffaella CARZANIGA (826 Velká Británie a Severní Irsko), Tohru TAKAKI (826 Velká Británie a Severní Irsko), Lucy M. COLLINSON (826 Velká Británie a Severní Irsko), Eric C. GREENE (840 Spojené státy), Lumír KREJČÍ (203 Česká republika, garant, domácí) a Simon J. BOULTON (826 Velká Británie a Severní Irsko)

Vydání

Molecular Cell, Cambridge, Cell Press, 2016, 1097-2765

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 14.714

Kód RIV

RIV/00216224:14110/16:00088541

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.molcel.2016.10.020

UT WoS

000389515500009

Klíčová slova anglicky

SINGLE-STRANDED-DNA; INDUCED FLUORESCENCE ENHANCEMENT; CANCER TUMOR-SUPPRESSOR; REPLICATION PROTEIN-A; HOMOLOGOUS RECOMBINATION; SACCHAROMYCES-CEREVISIAE; MAMMALIAN-CELLS; COMPLEX; REPAIR; PROMOTES

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 1. 2017 11:55, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Central to homologous recombination in eukaryotes is the RAD51 recombinase, which forms helical nucleoprotein filaments on single-stranded DNA (ssDNA) and catalyzes strand invasion with homologous duplex DNA. Various regulatory proteins assist this reaction including the RAD51 paralogs. We recently discovered that a RAD51 paralog complex from C. elegans, RFS-1/RIP-1, functions predominantly downstream of filament assembly by binding and remodeling RAD-51-ssDNA filaments to a conformation more proficient for strand exchange. Here, we demonstrate that RFS-1/RIP-1 acts by shutting down RAD-51 dissociation from ssDNA. Using stopped-flow experiments, we show that RFS-1/RIP-1 confers this dramatic stabilization by capping the 50 end of RAD-51-ssDNA filaments. Filament end capping propagates a stabilizing effect with a 5'-> 3' polarity approximately 40 nucleotides along individual filaments. Finally, we discover that filament capping and stabilization are dependent on nucleotide binding, but not hydrolysis by RFS-1/RIP-1. These data define the mechanism of RAD51 filament remodeling by RAD51 paralogs.

Návaznosti

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

Citovat

TAYLOR, Martin R.G., Mário ŠPÍREK, Chu Jian MA, Raffaella CARZANIGA, Tohru TAKAKI, Lucy M. COLLINSON, Eric C. GREENE, Lumír KREJČÍ a Simon J. BOULTON. A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling. Molecular Cell. Cambridge: Cell Press, 2016, roč. 64, č. 5, s. 926-939. ISSN 1097-2765. Dostupné z: https://dx.doi.org/10.1016/j.molcel.2016.10.020.

@article{1367405,
   author = {Taylor, Martin R.G. and Špírek, Mário and Ma, Chu Jian and Carzaniga, Raffaella and Takaki, Tohru and Collinson, Lucy M. and Greene, Eric C. and Krejčí, Lumír and Boulton, Simon J.},
   article_location = {Cambridge},
   article_number = {5},
   doi = {http://dx.doi.org/10.1016/j.molcel.2016.10.020},
   keywords = {SINGLE-STRANDED-DNA; INDUCED FLUORESCENCE ENHANCEMENT; CANCER TUMOR-SUPPRESSOR; REPLICATION PROTEIN-A; HOMOLOGOUS RECOMBINATION; SACCHAROMYCES-CEREVISIAE; MAMMALIAN-CELLS; COMPLEX; REPAIR; PROMOTES},
   language = {eng},
   issn = {1097-2765},
   journal = {Molecular Cell},
   title = {A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling},
   volume = {64},
   year = {2016}
}

TY  - JOUR
ID  - 1367405
AU  - Taylor, Martin R.G. - Špírek, Mário - Ma, Chu Jian - Carzaniga, Raffaella - Takaki, Tohru - Collinson, Lucy M. - Greene, Eric C. - Krejčí, Lumír - Boulton, Simon J.
PY  - 2016
TI  - A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling
JF  - Molecular Cell
VL  - 64
IS  - 5
SP  - 926-939
EP  - 926-939
PB  - Cell Press
SN  - 10972765
KW  - SINGLE-STRANDED-DNA
KW  - INDUCED FLUORESCENCE ENHANCEMENT
KW  - CANCER TUMOR-SUPPRESSOR
KW  - REPLICATION PROTEIN-A
KW  - HOMOLOGOUS RECOMBINATION
KW  - SACCHAROMYCES-CEREVISIAE
KW  - MAMMALIAN-CELLS
KW  - COMPLEX
KW  - REPAIR
KW  - PROMOTES
N2  - Central to homologous recombination in eukaryotes is the RAD51 recombinase, which forms helical nucleoprotein filaments on single-stranded DNA (ssDNA) and catalyzes strand invasion with homologous duplex DNA. Various regulatory proteins assist this reaction including the RAD51 paralogs. We recently discovered that a RAD51 paralog complex from C. elegans, RFS-1/RIP-1, functions predominantly downstream of filament assembly by binding and remodeling RAD-51-ssDNA filaments to a conformation more proficient for strand exchange. Here, we demonstrate that RFS-1/RIP-1 acts by shutting down RAD-51 dissociation from ssDNA. Using stopped-flow experiments, we show that RFS-1/RIP-1 confers this dramatic stabilization by capping the 50 end of RAD-51-ssDNA filaments. Filament end capping propagates a stabilizing effect with a 5'-> 3' polarity approximately 40 nucleotides along individual filaments. Finally, we discover that filament capping and stabilization are dependent on nucleotide binding, but not hydrolysis by RFS-1/RIP-1. These data define the mechanism of RAD51 filament remodeling by RAD51 paralogs.
ER  -

TAYLOR, Martin R.G., Mário ŠPÍREK, Chu Jian MA, Raffaella CARZANIGA, Tohru TAKAKI, Lucy M. COLLINSON, Eric C. GREENE, Lumír KREJČÍ a Simon J. BOULTON. A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling. \textit{Molecular Cell}. Cambridge: Cell Press, 2016, roč.~64, č.~5, s.~926-939. ISSN~1097-2765. Dostupné z: https://dx.doi.org/10.1016/j.molcel.2016.10.020.

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