BENDL, J., Jan ŠTOURAČ, Eva ŠEBESTOVÁ, Ondřej VÁVRA, Miloš MUSIL, Jan BREZOVSKÝ a Jiří DAMBORSKÝ. HotSpot Wizard 2.0: Automated Design of Site-Specific Mutations and Smart Libraries in Protein Engineering. Nucleic Acids Research. Oxford University Press, 2016, roč. 44, W1, s. "W479"-"W487", 9 s. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkw416. |
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@article{1367555, author = {Bendl, J. and Štourač, Jan and Šebestová, Eva and Vávra, Ondřej and Musil, Miloš and Brezovský, Jan and Damborský, Jiří}, article_number = {W1}, doi = {http://dx.doi.org/10.1093/nar/gkw416}, keywords = {MULTIPLE SEQUENCE ALIGNMENTS; FUNCTIONALLY IMPORTANT RESIDUES; FOCUSED DIRECTED EVOLUTION; SATURATION MUTAGENESIS; CORRELATED MUTATIONS; ENZYME PROPERTIES; THERMOSTABILITY; IDENTIFICATION; CONSERVATION; BINDING}, language = {eng}, issn = {0305-1048}, journal = {Nucleic Acids Research}, title = {HotSpot Wizard 2.0: Automated Design of Site-Specific Mutations and Smart Libraries in Protein Engineering}, url = {https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bendl_2016NAR.pdf}, volume = {44}, year = {2016} }
TY - JOUR ID - 1367555 AU - Bendl, J. - Štourač, Jan - Šebestová, Eva - Vávra, Ondřej - Musil, Miloš - Brezovský, Jan - Damborský, Jiří PY - 2016 TI - HotSpot Wizard 2.0: Automated Design of Site-Specific Mutations and Smart Libraries in Protein Engineering JF - Nucleic Acids Research VL - 44 IS - W1 SP - "W479"-"W487" EP - "W479"-"W487" PB - Oxford University Press SN - 03051048 KW - MULTIPLE SEQUENCE ALIGNMENTS KW - FUNCTIONALLY IMPORTANT RESIDUES KW - FOCUSED DIRECTED EVOLUTION KW - SATURATION MUTAGENESIS KW - CORRELATED MUTATIONS KW - ENZYME PROPERTIES KW - THERMOSTABILITY KW - IDENTIFICATION KW - CONSERVATION KW - BINDING UR - https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bendl_2016NAR.pdf L2 - https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bendl_2016NAR.pdf N2 - HotSpot Wizard 2.0 is a web server for automated identification of hot spots and design of smart libraries for engineering proteins’ stability, catalytic activity, substrate specificity and enantioselectivity. The server integrates sequence, structural and evolutionary information obtained from 3 databases and 20 computational tools. Users are guided through the processes of selecting hot spots using four different protein engineering strategies and optimizing the resulting library’s size by narrowing down a set of substitutions at individual randomized positions. The only required input is a query protein structure. The results of the calculations are mapped onto the protein’s structure and visualized with a JSmol applet. HotSpot Wizard lists annotated residues suitable for mutagenesis and can automatically design appropriate codons for each implemented strategy. Overall, HotSpot Wizard provides comprehensive annotations of protein structures and assists protein engineers with the rational design of site-specific mutations and focused libraries. It is freely available at http://loschmidt.chemi.muni.cz/hotspotwizard. ER -
BENDL, J., Jan ŠTOURAČ, Eva ŠEBESTOVÁ, Ondřej VÁVRA, Miloš MUSIL, Jan BREZOVSKÝ a Jiří DAMBORSKÝ. HotSpot Wizard 2.0: Automated Design of Site-Specific Mutations and Smart Libraries in Protein Engineering. \textit{Nucleic Acids Research}. Oxford University Press, 2016, roč.~44, W1, s.~''W479''-''W487'', 9 s. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkw416.
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