Enzyme Based Test Stripes for the Visual or Photographic
Detection and Quantitation of Gaseous Sulfur Mustard

J 2016

Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard

BIDMANOVÁ, Šárka, M.S. STEINER, Martina STEPAN, Kamila VYMAZALOVA, M.A. GRUBER et. al.

Základní údaje

Originální název

Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard

Autoři

BIDMANOVÁ, Šárka (203 Česká republika, domácí), M.S. STEINER (276 Německo), Martina STEPAN (203 Česká republika), Kamila VYMAZALOVA (203 Česká republika), M.A. GRUBER (276 Německo), A. DUERKOP (276 Německo), Jiří DAMBORSKÝ (203 Česká republika, garant, domácí), Zbyněk PROKOP (203 Česká republika, domácí) a O.S. WOLFBEIS (276 Německo)

Vydání

ANALYTICAL CHEMISTRY, 2016, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.320

Kód RIV

RIV/00216224:14310/16:00087645

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1021/acs.analchem.6b01272

UT WoS

000377631000069

Klíčová slova anglicky

PH; OXYGEN; TEMPERATURE; SMARTPHONE; SENSOR

Štítky

AKR, rivok

Změněno: 5. 4. 2017 17:55, Ing. Andrea Mikešková

Anotace

V originále

Sulfur mustard is a chemical agent of high military and terroristic significance. No effective antidote does exist, and sulfur mustard can be fairly easily produced in large quantity. Rapid field testing of sulfur mustard is highly desirable. Existing analytical devices for its detection are available but can suffer from low selectivity, laborious sample preparation, and/or the need for complex instrumentation. We describe a new kind of test stripe for rapid detection of gaseous sulfur mustard that is based on its degradation by the enzyme haloalkane dehalogenase that is accompanied by a change of local pH. This change can be detected using pH indicators contained in the stripes whose color changes from blue-green to yellow within 10 min. In addition to visual read-out, we also demonstrate quantitative reflectometric readout by using a conventional digital camera based on red-green-blue data acquisition. Organic haloalkanes, such as 1,2-dichloroethane, have a negligible interfering effect. The visual limit of detection is 20 µg/L, and the one for red-green-blue read-out is as low as 3 µg/L. The assays have good reproducibility +/- 6 % and +/- 2 % for inter-day assays and intra-day assays, respectively. The stripes can be stored for at least 6 months without loss of function. They are disposable and can be produced fairly rapidly and at low costs. Hence, they represent a promising tool for in-field detection of sulfur mustard.

Návaznosti

EE2.3.20.0183, projekt VaV

Název: Centrum experimentální biomedicíny

LM2011028, projekt VaV

Název: RECETOX ? Národní infrastruktura pro výzkum toxických látek v prostředí

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX Národní infrastruktura pro výzkum toxických látek v prostředí

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

TA04021380, projekt VaV

Název: Biosenzor pro monitorování toxických látek v životním prostředí

Investor: Technologická agentura ČR, Biosenzor pro monitorování toxických látek v životním prostředí

Citovat

BIDMANOVÁ, Šárka, M.S. STEINER, Martina STEPAN, Kamila VYMAZALOVA, M.A. GRUBER, A. DUERKOP, Jiří DAMBORSKÝ, Zbyněk PROKOP a O.S. WOLFBEIS. Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard. ANALYTICAL CHEMISTRY. 2016, roč. 88, č. 11, s. 6044-6049. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.6b01272.

@article{1367557,
   author = {Bidmanová, Šárka and Steiner, M.S. and Stepan, Martina and Vymazalova, Kamila and Gruber, M.A. and Duerkop, A. and Damborský, Jiří and Prokop, Zbyněk and Wolfbeis, O.S.},
   article_number = {11},
   doi = {http://dx.doi.org/10.1021/acs.analchem.6b01272},
   keywords = {PH; OXYGEN; TEMPERATURE; SMARTPHONE; SENSOR},
   language = {eng},
   issn = {0003-2700},
   journal = {ANALYTICAL CHEMISTRY},
   title = {Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard},
   url = {https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bidmanova_2016AC.pdf},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1367557
AU  - Bidmanová, Šárka - Steiner, M.S. - Stepan, Martina - Vymazalova, Kamila - Gruber, M.A. - Duerkop, A. - Damborský, Jiří - Prokop, Zbyněk - Wolfbeis, O.S.
PY  - 2016
TI  - Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard
JF  - ANALYTICAL CHEMISTRY
VL  - 88
IS  - 11
SP  - 6044-6049
EP  - 6044-6049
SN  - 00032700
KW  - PH
KW  - OXYGEN
KW  - TEMPERATURE
KW  - SMARTPHONE
KW  - SENSOR
UR  - https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2016/05/Bidmanova_2016AC.pdf
N2  - Sulfur mustard is a chemical agent of high military and terroristic significance. No effective antidote does exist, and sulfur mustard can be fairly easily produced in large quantity. Rapid field testing of sulfur mustard is highly desirable. Existing analytical devices for its detection are available but can suffer from low selectivity, laborious sample preparation, and/or the need for complex instrumentation. We describe a new kind of test stripe for rapid detection of gaseous sulfur mustard that is based on its degradation by the enzyme haloalkane dehalogenase that is accompanied by a change of local pH. This change can be detected using pH indicators contained in the stripes whose color changes from blue-green to yellow within 10 min. In addition to visual read-out, we also demonstrate quantitative reflectometric readout by using a conventional digital camera based on red-green-blue data acquisition. Organic haloalkanes, such as 1,2-dichloroethane, have a negligible interfering effect. The visual limit of detection is 20 µg/L, and the one for red-green-blue read-out is as low as 3 µg/L. The assays have good reproducibility +/- 6 % and +/- 2 % for inter-day assays and intra-day assays, respectively. The stripes can be stored for at least 6 months without loss of function. They are disposable and can be produced fairly rapidly and at low costs. Hence, they represent a promising tool for in-field detection of sulfur mustard.
ER  -

BIDMANOVÁ, Šárka, M.S. STEINER, Martina STEPAN, Kamila VYMAZALOVA, M.A. GRUBER, A. DUERKOP, Jiří DAMBORSKÝ, Zbyněk PROKOP a O.S. WOLFBEIS. Enzyme Based Test Stripes for the Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard. \textit{ANALYTICAL CHEMISTRY}. 2016, roč.~88, č.~11, s.~6044-6049. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.6b01272.

Podrobný výpis o publikaci