Immunomodulatory Potency of Microcystin, an Important
Water-Polluting Cyanobacterial Toxin

J 2015

Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin

ADAMOVSKÝ, Ondřej, Zdena MOOSOVÁ, Michaela PEKAROVÁ, Amrita BASU, Pavel BABICA et. al.

Základní údaje

Originální název

Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin

Autoři

ADAMOVSKÝ, Ondřej (203 Česká republika, garant, domácí), Zdena MOOSOVÁ (203 Česká republika, domácí), Michaela PEKAROVÁ (703 Slovensko), Amrita BASU (356 Indie, domácí), Pavel BABICA (203 Česká republika, domácí), Lenka ŠVIHÁLKOVÁ ŠINDLEROVÁ (203 Česká republika), Lukáš KUBALA (203 Česká republika) a Luděk BLÁHA (203 Česká republika, domácí)

Vydání

Environmental Science & Technology, WASHINGTON, The American Chemical Society, 2015, 0013-936X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.393

Kód RIV

RIV/00216224:14310/15:00107138

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1021/acs.est.5b02049

UT WoS

000363348700054

Klíčová slova anglicky

Immunomodulatory Potency; Microcystin; Cyanobacterial Toxin

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2020 14:43, Mgr. Marie Šípková, DiS.

Anotace

V originále

Microcystins (MCs) are primarily hepatotoxins produced by cyanobacteria and are responsible for intoxication in humans and animals. There are many incidents of chronic exposure to MCs, which have been attributed to the inappropriate treatment of water supplies or contaminated food. Using RAW 264.7 macrophages, we showed the potency of microcystin-LR (MC-LR) to stimulate production of pro-inflammatory cytokines (tumor necrosis factor alpha and interleukin-6) as a consequence of fast nuclear factor kappa B and nitrogen-activated protein kinase activation. In contrast to other studies, the observed effects were not attributed to the intracellular inhibition of protein phosphatases 1/2A due to lack of specific transmembrane transporters for MCs. However, the MC-LR-induced activation of macrophages was effectively inhibited by a specific peptide that blocks signaling of receptors, which play a pivotal role in the innate immune responses. Taken together, we showed for the first time that MC-LR could interfere with macrophage receptors that are responsible for triggering the above-mentioned signaling pathways. These findings provide an interesting mechanistic explanation of some adverse health outcomes associated with toxic cyanobacteria and MCs.

Návaznosti

GP13-27695P, projekt VaV

Název: Metabolity sinic jako možné modulátory imunitního systému (Akronym: immunotox)

Investor: Grantová agentura ČR, Metabolity sinic jako možné modulátory immunitního systému

Citovat

ADAMOVSKÝ, Ondřej, Zdena MOOSOVÁ, Michaela PEKAROVÁ, Amrita BASU, Pavel BABICA, Lenka ŠVIHÁLKOVÁ ŠINDLEROVÁ, Lukáš KUBALA a Luděk BLÁHA. Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin. Environmental Science & Technology. WASHINGTON: The American Chemical Society, 2015, roč. 49, č. 20, s. 12457-12464. ISSN 0013-936X. Dostupné z: https://dx.doi.org/10.1021/acs.est.5b02049.

@article{1368566,
   author = {Adamovský, Ondřej and Moosová, Zdena and Pekarová, Michaela and Basu, Amrita and Babica, Pavel and Švihálková Šindlerová, Lenka and Kubala, Lukáš and Bláha, Luděk},
   article_location = {WASHINGTON},
   article_number = {20},
   doi = {http://dx.doi.org/10.1021/acs.est.5b02049},
   keywords = {Immunomodulatory Potency; Microcystin; Cyanobacterial Toxin},
   language = {eng},
   issn = {0013-936X},
   journal = {Environmental Science & Technology},
   title = {Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin},
   url = {http://pubs.acs.org/doi/abs/10.1021/acs.est.5b02049},
   volume = {49},
   year = {2015}
}

TY  - JOUR
ID  - 1368566
AU  - Adamovský, Ondřej - Moosová, Zdena - Pekarová, Michaela - Basu, Amrita - Babica, Pavel - Švihálková Šindlerová, Lenka - Kubala, Lukáš - Bláha, Luděk
PY  - 2015
TI  - Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin
JF  - Environmental Science & Technology
VL  - 49
IS  - 20
SP  - 12457-12464
EP  - 12457-12464
PB  - The American Chemical Society
SN  - 0013936X
KW  - Immunomodulatory Potency
KW  - Microcystin
KW  - Cyanobacterial Toxin
UR  - http://pubs.acs.org/doi/abs/10.1021/acs.est.5b02049
L2  - http://pubs.acs.org/doi/abs/10.1021/acs.est.5b02049
N2  - Microcystins (MCs) are primarily hepatotoxins produced by cyanobacteria and are responsible for intoxication in humans and animals. There are many incidents of chronic exposure to MCs, which have been attributed to the inappropriate treatment of water supplies or contaminated food. Using RAW 264.7 macrophages, we showed the potency of microcystin-LR (MC-LR) to stimulate production of pro-inflammatory cytokines (tumor necrosis factor alpha and interleukin-6) as a consequence of fast nuclear factor kappa B and nitrogen-activated protein kinase activation. In contrast to other studies, the observed effects were not attributed to the intracellular inhibition of protein phosphatases 1/2A due to lack of specific transmembrane transporters for MCs. However, the MC-LR-induced activation of macrophages was effectively inhibited by a specific peptide that blocks signaling of receptors, which play a pivotal role in the innate immune responses. Taken together, we showed for the first time that MC-LR could interfere with macrophage receptors that are responsible for triggering the above-mentioned signaling pathways. These findings provide an interesting mechanistic explanation of some adverse health outcomes associated with toxic cyanobacteria and MCs.
ER  -

ADAMOVSKÝ, Ondřej, Zdena MOOSOVÁ, Michaela PEKAROVÁ, Amrita BASU, Pavel BABICA, Lenka ŠVIHÁLKOVÁ ŠINDLEROVÁ, Lukáš KUBALA a Luděk BLÁHA. Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin. \textit{Environmental Science \&{} Technology}. WASHINGTON: The American Chemical Society, 2015, roč.~49, č.~20, s.~12457-12464. ISSN~0013-936X. Dostupné z: https://dx.doi.org/10.1021/acs.est.5b02049.

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