J 2016

Histopathology Image Analysis in Two Long-Term Animal Experiments with Helical Flow Total Artificial Heart

WOTKE, Jiří, Pavel HOMOLKA, Jaromír VASKU, Petr DOBŠÁK, Petra PALANOVÁ et. al.

Základní údaje

Originální název

Histopathology Image Analysis in Two Long-Term Animal Experiments with Helical Flow Total Artificial Heart

Autoři

WOTKE, Jiří (203 Česká republika, domácí), Pavel HOMOLKA (203 Česká republika, domácí), Jaromír VASKU (203 Česká republika), Petr DOBŠÁK (203 Česká republika, garant, domácí), Petra PALANOVÁ (203 Česká republika, domácí), Veronika MRKVICOVÁ (203 Česká republika, domácí), Petr KONEČNÝ (203 Česká republika, domácí), Vladimír SOŠKA (203 Česká republika, domácí), Michal POHANKA (203 Česká republika, domácí), Marie NOVÁKOVÁ (203 Česká republika, domácí), Terumi YURIMOTO (392 Japonsko), Itsuro SAITO (392 Japonsko), Yusuke INOUE (392 Japonsko), Takashi ISOYAMA (392 Japonsko) a Yusuke ABE (392 Japonsko)

Vydání

Artificial organs, Hoboken, Wiley-Blackwell, 2016, 0160-564X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.403

Kód RIV

RIV/00216224:14110/16:00093337

Organizační jednotka

Lékařská fakulta

DOI

UT WoS

000389465300012

Klíčová slova anglicky

Total artificial heart; Helical flow; Titanium alloy; Microparticles; Histopathology

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 14. 11. 2017 10:43, Soňa Böhmová

Anotace

V originále

Histopathological analysis can provide important information in long-term experiments with total artificial heart (TAH). Recently, a new type of blood pump, the helical flow total artificial heart (HF-TAH) was developed. This study aimed to investigate the changes in selected vital organs in animal experiments with implanted HF-TAH. Samples from lung, liver, and kidneys from two female goats (No. 1301 and No. 1304) with implanted HF-TAH were analyzed. Tissue samples were fixed in 10% formaldehyde and 4 mm thick transverse sections were stained with hematoxylin-eosin (HE). Additional staining was done for detection of connective tissue (Masson-Goldner stain) and for detection of iron (hemosiderin) deposits (Perls stain). Sections were scanned at 1003 and 5003 magnification with a light microscope. Experiment no. 1301 survived 100 days (cause of termination was heavy damage of the right pump); experimental goat no. 1304 survived 68 days and was sacrificed due to severe right hydrodynamic bearing malfunction. Histopathological analysis of liver samples proved signs of chronic venostasis with limited focal necrotic zones. Dilated tubules, proteinaceous material in tubular lumen, and hemosiderin deposits were detected in kidney samples. Contamination of the organs by embolized micro-particles was suspected at the autopsy after discovery of visible damage (scratches) of the pump impeller surface (made from titanium alloy) in both experiments. Sporadic deposits of foreign micro-particles (presumably titanium) were observed in most of the analyzed parenchymal organs. However, the described deposits were not in direct connection with inflammatory reactions in the analyzed tissues. Histopathological analysis showed the presence of minimal contamination of the lung, kidney, and liver tissue samples by foreign material (titanium very likely). The analysis showed only limited pathological changes, especially in liver and kidneys, which might be attributed to the influence of artificial perfusion often observed in chronic TAH experiments.